Spectrofluorometric Determination of Total Bilirubin in Human Serum Samples Using Tetracycline-Eu3+

Abstract

The measurement of total bilirubin in serum is one of the most frequently performed tests in clinical analysis. A new spectrofluorometric method was developed for the determination of trace amounts of total bilirubin. Using tetracycline-Eu 3+ as a fluorescent probe, bilirubin can remarkably reduce the fluorescence intensity of the tetracycline-Eu 3+ complex, and the reduced fluorescence intensity of Eu 3+ ion is in proportion to the concentration of bilirubin. The optimum conditions for the determination of bilirubin were also investigated. This method is simple, practical, and relatively free of interference from coexisting substances. It can be successfully applied to assess total bilirubin in serum samples. We also compared the developed method with a modified Jendrassik-Grof method in clinical analysis.

Polymeric copper(ii) and dimeric oxovanadium(v) complexes of amide–imine conjugate: bilirubin recognition and green catalysis

“…For example, recognition of unconjugated, conjugated and total bilirubin has been performed using a D-glucuronic acid appended polymer (PF-Ph-GlcA), 96 modied MOF (UIO-66-PSM), 97 Zr-MOF: Eu 3+ probe, 98 HSA-AuNCs, 99 L-cysteine modulated-CuNCs, 100 BSA-CuNCs, 101 Fe(III)-modulated BSA-CuNCs, 102 2,2 ′ -((1E,1 ′ E)-(6-bromopyridine-2,3-diyl) bis-(azanylylidene)) bis-(methanylylidenediphenol), 103 ber optic uorescent biosensors, 80,104 Ru(bipy) 3 2+ , 105 S,N-doped CDs 106 and 4,4,4-triuoro-1-(thiophen-2-yl)-butane-1,3-dione europium(III) complex (Eu(tta) 3 ), 107 pyrene based Schiff-base, 108 polystyrene based probe, 109 and N-doped graphene QDs/CoFe 2 O 4 -DOPA/ H 2 O 2 system. 110 A careful review of the literature indicates that very few metal complex-based bilirubin sensors 111,112 are available that function via a uorescence quenching mechanism, a highly undesirable protocol. Herein, we explored a 1D copper(II) coordination polymer (C1) for the recognition of bilirubin via "turn-on uorescence" without any signicant interference from competing bio-molecules.…”

“…A careful review of the literature indicates that very few metal complex-based bilirubin sensors 111,112 are available that function via a fluorescence quenching mechanism, a highly undesirable protocol. Herein, we explored a 1D copper( ii ) coordination polymer ( C1 ) for the recognition of bilirubin via “turn-on fluorescence” without any significant interference from competing bio-molecules.…”