Spectrofluorometrical Determination of Vitamin B₁ in Different Matrices Using Box‐Behnken Designed Pipette Tip Solid Phase Extraction by a Carbon Nanotube Sorbent

Abstract

This study describes development of a method for spectrofluorometrical determination of thiamine (vitamin B 1 ) in vitamin tablets, cactus fruits, multivitamin syrups, and human plasma after its extraction by pipette tip micro-solid phase extraction using carbon nanotube as sorbent. A response surface methodology was used for experimental design to obtain optimal response. Ten variables including pH of the sample solution, type and volume of eluent solvent, volume of sample solution, effect of ionic strength, concentration of K 3 [Fe(CN) 6 ] (as derivatizing agent), concentration of NaOH, number of cycles of extraction and elution and amount of sorbent were regarded as factors in the optimization study by Box-Behnken design. Figures of merit were calculated as: detection limit; 0.16 μg L À 1 , enrichment factor; 91, linear calibration curve in the range of 0.5-130.0 μg L À 1 ; and reproducibility (as RSD %) better than 2.4%. The method was applied to the determination of different real samples successfully.[a] Dr.

Application of a novel deep eutectic solvent modified carbon nanotube for pipette-tip micro solid phase extraction of 6-mercaptopurine

“…Due to their high adsorption capacity and rapid desorption, CNTs became attractive for a variety of analytical applications, especially for micro-scale gas and liquid-phase analysis. Moreover, the ease of surface functionalization made CNTs very flexible for the extraction of almost any analyte of interest [ 16 , 17 ].…”