Spectrometric analysis, chemical constituents and cytotoxic evaluation of Astragalus sieberi DC. (Fabaceae)

Salem, Maha; Farid, Mai M.; El-Shabrawy, Mona; Mohammed, Reda S.; Hussein, Sameh R.; Marzouk, Mona M.

doi:10.1016/j.sciaf.2019.e00221

Cited by 12 publications

(17 citation statements)

References 31 publications

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“…The MS spectrum of compound 1 showed a molecular ion peak at m/z 341.29 which was tentatively characterized as caffeic acid-O-hexoside, based on the loss of hexoside (−162.14 Da) and CO 2 (−44.01 Da) moieties and the presence of characteristic fragments at m/z 179.15 [M–H-hexoside] − and m/z 135.14 [M-H-hexoside-CO 2 ] − 34 . Furthermore, the MS spectra of compounds 4 , 6 , 7 and 15 displayed base peak ions [M-H] − at m/z 169.11, 179.15, 197.17, and 193.18, respectively in addition to their characteristic decarboxylated fragments.…”

Section: Discussionmentioning

confidence: 99%

“…For compounds 7 and 15 another fragment arising from elimination of methyl group (15.03 Da) was readily observed in their MS 2 spectra at m/z 182.14 and 178.15, respectively. The compounds were accordingly identified as gallic acid (compound 4 ), caffeic acid (compound 6 ), syringic acid (compound 7 ), and ferulic acid (compound 15 ), respectively 34 , 35 . Meanwhile, compound 5 exhibited an intense base peak [M–H] − at m/z 353.3 with diagnostic fragments at m/z 179.15 and m/z 191.16 due to caffeic acid and quinic acid, respectively and thus compound 5 was assigned as monocaffeoylquinic acid (chlorogenic acid) in accordance with the literature 35 , which indicates that caffeic acid is frequently associated with quinic acid.…”

Section: Discussionmentioning

confidence: 99%

“…Kaempferol-O-rutinoside-O-arabinoside (compound 9 ) and astragalin (compound 21 ) produced a characteristic fragment ion at m/z 285.23 due to loss of C 17 H 28 O 13 and C 6 H 10 O 5 , respectively. These compounds together with kaempferol (compound 38 ) showed fragment ions at m/z 151.1 and 133.12 due to Retro Diels–Alder (RDA) reaction 34 , 39 . Similarly, isorhamnetin-O-rutinoside-O-arabinoside (compound 10 ), isorhamnetin-3-O-rutinoside (compound 14 ) and isorhamnetin-3-O-B-D-glucoside (compound 23 ) showed a distinct fragment ion at m/z 315.26 due to elimination of C 17 H 28 O 13 , C 12 H 20 O 9 and C 6 H 10 O 5 , respectively.…”

Section: Discussionmentioning

confidence: 99%

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Alleviation of liver cirrhosis and associated portal-hypertension by Astragalus species in relation to their UPLC-MS/MS metabolic profiles: a mechanistic study

Ibrahim

El–Mezayen

El‐Banna

2022

Sci Rep

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Liver cirrhosis is a late-stage liver disease characterized by excessive fibrous deposition triggering portal-hypertension (PH); the prime restrainer for cirrhosis-related complications. Remedies that can dually oppose hepatic fibrosis and lower PH, may prevent progression into decompensated-cirrhosis. Different Astragalus-species members have shown antifibrotic and diuretic actions with possible subsequent PH reduction. However, A.spinosus and A.trigonus were poorly tested for eliciting these actions. Herein, A.spinosus and A.trigonus roots and aerial parts extracts were subjected to comprehensive metabolic-fingerprinting using UHPLC-MS/MS resulting in 56 identified phytoconstituents, followed by chemometric untargeted analysis that revealed variable metabolic profiles exemplified by different species and organ types. Consequently, tested extracts were in-vivo evaluated for potential antifibrotic/anticirrhotic activity by assessing specific markers. The mechanistic prospective to induce diuresis was investigated by analyzing plasma aldosterone and renal-transporters gene-expression. Serum apelin and dimethylarginine-dimethylaminohydrolase-1 were measured to indicate the overall effect on PH. All extracts amended cirrhosis and PH to varying extents and induced diuresis via different mechanisms. Further, An OPLS model was built to generate a comprehensive metabolic-profiling of A.spinosus and A.trigonus secondary-metabolites providing a chemical-based evidence for their efficacious consistency. In conclusion, A.spinosus and A.trigonus organs comprised myriad pharmacologically-active constituents that act synergistically to ameliorate cirrhosis and associated PH.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Alleviation of liver cirrhosis and associated portal-hypertension by Astragalus species in relation to their UPLC-MS/MS metabolic profiles: a mechanistic study

Ibrahim

El–Mezayen

El‐Banna

2022

Sci Rep

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“…The unsaponified matter and fatty acid methyl esters were subjected to GC/MS analysis based on the method described by Salem et al 18 Investigation of DAME Isolation and structure elucidation DAME of M. indicus was applied to a polyamide column (100 x 5 cm) starting with H2O as eluent then decreasing the polarity by increasing the concentration of MeOH. Collectively, 54 fractions were obtained, each of about 300 mL.…”

Section: Gc-ms Analysismentioning

confidence: 99%

Chemical Profiling, In Vivo Antioxidant Effect and Antimutagenic Evaluation of Melilotus indicus (L.) All. Extracts

2021

TJNPR

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derivatives. 10,11 Type II diabetes mellitus (DM) incidence is rising globally and considered one of the major human health worries. Thus, intervention before the appearance of disease through edible vegetables may help in prevention and controlling its complications. 12 Oxidative stress and free radical formation intensively upswing in diabetes. 13 Accumulation of reactive oxygen species (ROS) due to hyperglycemia causes DNA damage and results in mutagenicity of bone marrow and sperms. 14 Potential antigenotoxicity was achieved by medicinal plants via their phenolic constituents through several mechanisms including their antioxidative stress effect. 15 No study has been carried out on the spectrometric analysis, in vivo anti-diabetic effect and antimutagenic evaluation of M. indicus. Therefore, the present study aims to identify and profiling the phytoconstituents of M. indicus extracts using GC-MS and LC-ESI-MS techniques. Also, their antioxidant, antidiabetic and antimutagenic effects in alloxan induced diabetic rats were evaluated Material and Methods Plant material and extractionThe whole plants of M. indicus were freshly collected on 14 February 2016 as weeds of cultivation, 6 th October City, Giza, Egypt. The plant sample was authenticated by Prof. Dr. M. M. Marzouk and the voucher specimen (N2_14216) was prepared and has been deposited in the herbarium of National Research Centre. The air-dried plant sample (056 g) was ground and extracted with 70% MeOH/H2O then 50% MeOH/H2O for several times till exhaustion, at room temperature. The combined extract was distilled off under reduced

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“…1). They were identified as kaempferol (1) [29], kaempferol -3-O-glucoside (2) [30], kaempferol -3,7-di-Oglucoside (3) [31], luteolin 7-O-glucoside ) 4) [32][33], isoorientin (5) [34], isoorientin 7-O-glucoside (lutonarin) (6) [35], caffeic acid (7) [36], emodin (8) [37], apigenin 7-O-glucoside (9) [36] and isovitexin-7-O-glucoside (saponarin) (10) [38]. Compounds (2, 3, 6-8) were isolated for the first time from the investigated species while the remaining compounds were reported previously from the aerial parts of A. aestivus [33].…”

Section: Identification Of the Isolated Compoundsmentioning

confidence: 99%

Chemical Analysis and Cytotoxic Evaluation of Asphodelus aestivus Brot. Flowers

et al. 2021

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Asphodelus species are among many plants used in traditional medicine, they are used by native peoples for various pathologies. Asphodelus aestivus Brot. is one of Asphodelus species which is considered an important wild medicinal plant growing mainly at Mediterranean region. The present study aims to investigate the chemical constituents and cytotoxic evaluation of A. aestivus flowers. Petroleum ether extract was analyzed by GC/MS assay which revealed the characterization of twenty nine compounds, representing 93.13% of total extract. Also, the chromatographic investigation of the hydromethanolic extract led to the isolation of ten phenolics. They were identified as kaempferol (1), kaempferol 3-Oglucoside (2), kaempferol 3,7-di-O-glucoside (3), luteolin 7-O-glucoside ) 4), isoorientin (5), isoorientin 7-O-glucoside (lutonarin) (6), caffeic acid ( 7) emodin ( 8), apigenin 7-O-glucoside (9) and isovitexin-7-O-glucoside (saponarin) (10), from which, five compounds ((2, 3, 6-8) have been isolated for the first time from A. aestivus. Furthermore, LC-ESI-MS chemical profiling managed to detect the minor contents and led to the annotation of thirteen additional metabolites. In addition, the petroleum ether and the hydromethanolic extracts were investigated against four human carcinoma cell lines; HepG2, HCT-116, MCF-7 and A549 at concentration of 100 mg/mL. The results showed moderate % of inhibition against MCF-7 (63.8 and 68.4) followed by HepG2 (52.3 and 65.7), respectively. Both extracts showed weak inhibition (5.7-14.3%) on HCT-116 and A549. To the best of our knowledge, this is considered the first report of the chemical analysis and biological evaluation of A. aestivus flowers.

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Spectrometric analysis, chemical constituents and cytotoxic evaluation of Astragalus sieberi DC. (Fabaceae)

Cited by 12 publications

References 31 publications

Alleviation of liver cirrhosis and associated portal-hypertension by Astragalus species in relation to their UPLC-MS/MS metabolic profiles: a mechanistic study

Alleviation of liver cirrhosis and associated portal-hypertension by Astragalus species in relation to their UPLC-MS/MS metabolic profiles: a mechanistic study

Chemical Profiling, In Vivo Antioxidant Effect and Antimutagenic Evaluation of Melilotus indicus (L.) All. Extracts

Chemical Analysis and Cytotoxic Evaluation of Asphodelus aestivus Brot. Flowers

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