Spectrophotometric Assay of Metoclopramide Hydrochloride in Pharmaceutical Preparations via Arsenazo III-Cerium (III) Reaction

Abstract

A simple, rapid, accurate and precise spectrophotometric method is proposed for determining metoclopramide-hydrochloride (MCPH) in pure form and in pharmaceutical preparations .The method is based on oxidation-reduction reaction between metoclopramide-hydrochloride and cerium (IV) ion, then the subsequent reaction of cerium (III) and arsenazo III reagent in acidic medium to produce a blue-violet complex which is stable, water-soluble and has a maximum absorption at 654nm with a molar absorptivity of 9.36x10 4 l.mol-1 .cm-1 and Sandell's sensitivity of 0.0038 µg.cm-2. Beer's law is obeyed in concentration range from 1-30 µg metoclopramide-hydrochloride in final volume of 25 ml, (0.04-1.2) ppm with a relative standard deviation (RSD) (±1.80%-±3.98%) and the limit of detection (LOD) is 0.0095 µg/ml and the limit of quantitation (LOQ) is 0.0317 µg/ml. The method is suitable for the determination of metoclopramidehydrochloride in the presence of other ingredients that are usually present in dosage forms and the recoveries are obtained in the range of 97.6-101.0 %. The proposed method has been applied successfully to the determination of the intended compound in its pharmaceutical preparations (tablet and injection).

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“…22 Effect of solution temperature Effect of several temperatures (10-40 o C) that found the higher stability of color product and give best absorption at 25℃ temperatures. Thus when using high temperature gives low stability of color product and absorption because of partial dissociation of colored products 23,24 as appeared in (Figure 4).…”