Spectrophotometric assay of phenolphthalein in biological fluids

Morris, Sarah Margaret; Powell, Don W.

doi:10.1016/0003-2697(79)90757-7

Cited by 8 publications

(2 citation statements)

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“…Colorimetry was used for the estimation of phenolphthalein in serum and urine by Morris and Powell . A comparative study of reversed‐phase liquid chromatography (recommended in the U.S. Pharmacopeia) and colorimetry (recommended in the British Pharmacopeia) for the analysis of phenolphthalein in various commercial preparations was made by Torrado et al.…”

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confidence: 99%

Detection and Quantitation of Trace Phenolphthalein (in Pharmaceutical Preparations and in Forensic Exhibits) by Liquid Chromatography–Tandem Mass Spectrometry, a Sensitive and Accurate Method

Sharma

Lahiri

2012

Journal of Forensic Sciences

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Phenolphthalein, an acid-base indicator and laxative, is important as a constituent of widely used weight-reducing multicomponent food formulations. Phenolphthalein is an useful reagent in forensic science for the identification of blood stains of suspected victims and for apprehending erring officials accepting bribes in graft or trap cases. The pink-colored alkaline hand washes originating from the phenolphthalein-smeared notes can easily be determined spectrophotometrically. But in many cases, colored solution turns colorless with time, which renders the genuineness of bribe cases doubtful to the judiciary. No method is known till now for the detection and identification of phenolphthalein in colorless forensic exhibits with positive proof. Liquid chromatography-tandem mass spectrometry had been found to be most sensitive, accurate method capable of detection and quantitation of trace phenolphthalein in commercial formulations and colorless forensic exhibits with positive proof. The detection limit of phenolphthalein was found to be 1.66 pg/L or ng/mL, and the calibration curve shows good linearity (r(2) = 0.9974).

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confidence: 99%

Detection and Quantitation of Trace Phenolphthalein (in Pharmaceutical Preparations and in Forensic Exhibits) by Liquid Chromatography–Tandem Mass Spectrometry, a Sensitive and Accurate Method

Sharma

Lahiri

2012

Journal of Forensic Sciences

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“…This has on occasions led to patients being wrongly accused of sur reptitious laxative abuse and thus it was de sirable for more specific tests to be devel oped. While the tests we have mentioned are qualitative, a quantitative spectrophotometric assay for phenolphthalein in serum and urine has been developed [95] and shown to be sensitive in 10~5 M phenolphthalein. Both the free and conjugated forms can be measured.…”

Section: Chemical Detection Diphenolic Anthraquinone and Vegetablementioning

confidence: 99%