Spectrophotometric Assays for Antioxidant Enzymes in Plants

Elavarthi, Sathya; Martín, B.

doi:10.1007/978-1-60761-702-0_16

Cited by 180 publications

(95 citation statements)

References 14 publications

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“…SODs are metalloproteins that catalyze the dismutation reaction of two superoxide free radicals to one molecule of O2 and one molecule of H2O2 (Elavarthi and Martin, 2010). In our study, increasing NaCl concentration led to a significant increase in SOD activity of melon seedlings, that the highest activity was found in the…”

Section: Discussionmentioning

confidence: 47%

Multivariate Analysis as a Tool for Studying the Effects of Salinity in Different Melon Landraces at Germination Stage

Sarabi

Bolandnazar

Ghaderi

et al. 2016

Not Bot Horti Agrobo

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Although salt stress affects all growth stages of a plant, seed germination and seedling growth stages are known to be more sensitive for most plant species. The current research was carried out to find the most effective indicators when screening melons for salt tolerance. For this purpose, a factorial experiment as a completely randomized design was performed in order to investigate the effects of salinity (0, 20, 40, 60 and 80 mM NaCl) on seed germination parameters, hydrogen peroxide content, lipid peroxidation and some antioxidant enzymes (guaiacol peroxidase, ascorbate peroxidase and superoxide dismutase) in five Iranian melon landraces and one melon cultivar 'Galia' F1 at early seedling growth stage. The 'Galia' F1 was included for comparison as a salt-tolerant cultivar. The data collected from all salinity treatments across six landraces were used for analysis of variance and principal component analysis (PCA) using SPSS software as well as for obtaining Pearson's correlation coefficients (r) among all physiological parameters. Obtained results indicated that averaged all landraces across all treatments, increased NaCl significantly affected all the parameters measured. In all landraces, salt tolerance index (STI) decreased as the salt concentrations increased. The principal component analysis revealed variations among the traits and determined four main factors that explained 97.53% of the total variance. Simple correlation coefficient analyses showed the existence of significant positive and negative correlations among characteristics. Our study suggests that the salt tolerance index and principal component analysis can be used as accurate and easy indicators when screening melons for salt tolerance.

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Section: Discussionmentioning

confidence: 47%

Multivariate Analysis as a Tool for Studying the Effects of Salinity in Different Melon Landraces at Germination Stage

Sarabi

Bolandnazar

Ghaderi

et al. 2016

Not Bot Horti Agrobo

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“…The resulting supernatant was used to determine the protein content, as described by Bradford (1976). Specific enzyme activities for ascorbate peroxidase (APx), catalase (CAT), superoxide dismutase (SOD), and glutathione reductase (GR) were determined as described earlier (Elavarthi and Bjorn 2010;Gururani and others 2013b). To estimate the APx activity, the leaves were homogenized in 100 mM sodium phosphate buffer (pH 7.0) containing 5 mM ascorbate and 1 mM EDTA.…”

Section: Estimation Of Ros Scavenging Antioxidant Enzyme Activitiesmentioning

confidence: 99%

Transgenic Turfgrasses Expressing Hyperactive Ser599Ala Phytochrome A Mutant Exhibit Abiotic Stress Tolerance

Gururani

Ganesan

Song

et al. 2015

J Plant Growth Regul

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Turfgrasses are environmentally and recreationally valuable plants that are constantly subjected to various forms of stress in their artificial and natural habitats. Previously, it was shown that the transformation of a hyperactive mutant (Serine 599 Alanine, S599A) of oat phytochrome A in zoysia grass (Zoysia japonica) and creeping bentgrass (Agrostis stolonifera L.) resulted in superior quality turfgrass with improved shade tolerance response. We now examined the abiotic stress response of the transgenic turfgrasses expressing the hyperactive mutant S599A-PhyA. The transgenic S599A-PhyA plants subjected to high salinity and heavy metal toxicity stress exhibited higher chlorophyll content, lower hydrogen peroxide level, and higher proline accumulation than the controls. Furthermore, the anti-oxidative activities of four reactive oxygen species scavenging enzymes and the total biomass (above and below-ground) were higher in S599A-PhyA plants than in the controls under both the stress conditions. Moreover, higher photosynthetic efficiency (F v / F m ) of S599A-PhyA plants indicated healthier growth than the controls under stress conditions. Results suggest that the hyperactive mutant of oat phytochrome A confers abiotic stress tolerance in plants, and can be used to efficiently develop abiotic stress tolerant crops in future.

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“…In turn, environmental stress can cause a decline (Weidner et al 2007(Weidner et al , 2009b or an increase (Wróbel et al 2005;Weidner et al 2009a) in the content of phenolic compounds in a cell. Phenolic compounds can scavenge ROS (Amarowicz et al , 2004Negro et al 2003;Caillet et al 2006;Amarowicz and Weidner 2009), they form complexes with the metals, which catalyse oxygenation reactions and inhibit activity of oxidizing enzymes (Elavarthi and Martin 2010). Phenolic compounds include many secondary metabolites in plants that exhibit antioxidant properties (Oszmański 1995).…”

Section: Introductionmentioning

confidence: 99%

Changes in the composition of phenolic compounds and antioxidant properties of grapevine roots and leaves (Vitis vinifera L.) under continuous of long-term drought stress

2014

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Grapevine seedlings Vitis vinifera L. were grown in a greenhouse under optimum conditions (soil moisture ca 70 %) and under drought stress (soil moisture ca 30 %). Drought stress caused reduction in total phenolic compounds in grapevine leaves and roots, where were identified tree phenolic acids: caffeic acid, p-coumaric acid and ferulic acid. All acids found in leaves and roots occurred in the ester-bound form. Only caffeic acid in leaves appeared in the free and ester-bound form. Caffeic acid was present in the highest concentrations. The content of ferulic acid was the lowest in both tissues. The levels of all phenolic acids in leaves and roots decreased significantly under the drought stress. All the extracts from grapevine leaves and roots had antioxidative properties, but the antiradical activity of the extracts obtained from roots subjected to drought stress was lower to the control. The results of the analysis revealed that long-term drought stress caused a decrease in selected elements of secondary metabolism in such a different plant tissues that are the leaves and roots of the grapevine.

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Spectrophotometric Assays for Antioxidant Enzymes in Plants

Cited by 180 publications

References 14 publications

Multivariate Analysis as a Tool for Studying the Effects of Salinity in Different Melon Landraces at Germination Stage

Multivariate Analysis as a Tool for Studying the Effects of Salinity in Different Melon Landraces at Germination Stage

Transgenic Turfgrasses Expressing Hyperactive Ser599Ala Phytochrome A Mutant Exhibit Abiotic Stress Tolerance

Changes in the composition of phenolic compounds and antioxidant properties of grapevine roots and leaves (Vitis vinifera L.) under continuous of long-term drought stress

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