Spectrophotometric Determination and Biological Activity Study of Zn(II) using a Newly Synthesized Azo Reagent Derivative from 4,5-bis(4-methoxyphenyl) Imidazole

Abstract

A rapid and simple spectrophotometric method was submitted for Zn(II) determination using (E)-2-(4,5-bis(4-methoxyphenyl)-1H-imidazole-4-yl) diazenyl) benzoic acid as an analytical reagent. The suggested method is based on the chelation reaction between Zn(II) and the bidentate chelation ligand with a mole ratio of 1:2 (metal:ligand) with octahedral geometry. The maximum absorption of the formed violet complex was 560 nm, the linearity was in the concentration range of 0.5-15.0 µg mL-1. The relative standard deviation (n =10) was 0.149%. The interfering effect of diverse cations and anions was tested. To clarify the biological efficacy of the synthesized Zn(II) complex, an in vitro biological activity study was carried out against four kinds of bacterial strains, and the results showed promising inhibition activity.