Spectrophotometric Determination of Aldehydes in Alcohols

Development of a microfluidic paper-based analytical device for the determination of salivary aldehydes

“…This was not observed in the batch method [30], thus indicating that filter paper could be a possible source of interference. It was hypothesised that this background colour could be associated with the presence of aldehyde groups on the filter paper that have been produced as a result of aerial cellulose oxidation [35,36].…”

“…Previous studies by Sawicki et al [32] and Barary et al [30] have shown that a Step 2). Error bars are ± standard deviation for 1 σ n-1 (n = 11).…”

“…The accuracy of the µPAD was determined by recovery experiments which were conducted using spiked saliva samples and the results were compared with those produced by the standard reference method [30] (Table 2). Initial recovery studies using a separate µPAD for the blank (n=11) gave recoveries of 71 -118%.…”

“…In the analytical procedure for the standard reference method [30] 0.5 mL of a standard or sample were first added to 1 mL of MBTH solution. After 20 min, 1 mL of the Fe(III) solution was added to the solution mixture and left to react for a further 10 min.…”

“…The oxidizing agent, which consisted of 160 mM FeCl 3 was prepared by dissolving 216 mg FeCl 3 (SigmaAldrich) in 5 mL of 0.005 M HCl (Ajax FineChem). A 1.85 mM MBTH solution in 0.1 M hydrochloric acid and a 3.70 mM FeCl 3 solution wereused in conducting the spectrophotometric standard reference method[30].…”