Spectrophotometric Determination of Copper in Tellurium and Related Thermoelectric Compounds of the Bismuth Telluride Type with 2,9-Dimethyl-1,10-phenanthroline.

Nebesar, B

doi:10.1021/ac60216a022

Cited by 25 publications

(5 citation statements)

References 13 publications

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“…Neocuproine is a strong copper ion chelator (Nebesar, 1964), and in order to observe its effect on histone deacetylase activity, samples of the [3H] acetate-labeled complex were dialyzed against buffer A containing 3 mM neocuproine for 2 h at 4 °C and assayed. As controls, equal aliquots of the complex were treated in the same manner but without the addition of neocuproine.…”

Section: Resultsmentioning

confidence: 99%

Histone deacetylase from HeLa cells: properties of the high molecular weight complex

Hay¹,

Candido²

1983

Biochemistry

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In previous work [Hay, C. W., & Candido, E. P. M. (1983) J. Biol. Chem. 258, 3726-3734], we have shown that the histone deacetylase from HeLa cell nuclei is associated with a high molecular weight, nuclease-resistant complex. This complex was found to contain a variety of non-histone proteins, and indirect evidence for the importance of protein-protein interactions in the maintenance of its structure was obtained. In the present report, we examine the effects of beta-mercaptoethanol and neocuproine on the deacetylase complex and present data on the level of histone acetylation and the presence of satellite DNA sequences in this material. HeLa cell histone deacetylase complex partially dissociates in 10 mM beta-mercaptoethanol, resulting in a loss of non-histone proteins. The presence of 10 mM beta-mercaptoethanol during the micrococcal nuclease digestion of HeLa cell nuclei results in a greatly reduced yield of histone deacetylase complex, with a correspondingly large increase in the production of small oligonucleosomes and mononucleosomes. Histone deacetylase activity on endogenous labeled histone within the complex is strongly inhibited by either 1 or 10 mM beta-mercaptoethanol or 3 mM neocuproine. This loss of histone deacetylase activity does not seem to be due to an inactivation of the enzyme but appears to be a consequence of the disruption of the structure of the deacetylase complex itself. Histone H4 in the deacetylase complex prepared from HeLa cell nuclei by micrococcal nuclease digestion was more highly acetylated than H4 in bulk nucleosomes. Restriction enzyme analysis of the DNA associated with the histone deacetylase complex revealed neither an enrichment nor a depletion of major satellite sequences in this material.

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Section: Resultsmentioning

confidence: 99%

Histone deacetylase from HeLa cells: properties of the high molecular weight complex

Hay¹,

Candido²

1983

Biochemistry

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“…Solubilities were measured by equilibrating 100 mg of each solid complex with 10 ml of water, with stirring, for 24 hr at 25 _ 0.1~ After centrifuging at 50,000 g for 30 min, 5-ml aliquots of the supematant solutions were withdrawn, acidified to pH 3, and analyzed for acifluorfen by high-performance liquid chromatography (HPLC) and for metal ions by spectrophotometric or titrimetric methods (Bandemer and Schaible, 1944;Chenery, 1948;Hildebrand and Reilly, 1957;Nebesar, 1964). All the experiments were performed in triplicate.…”

Section: Solubility Measurementsmentioning

confidence: 99%

Interaction of the Herbicide Acifluorfen with Montmorillonite: Formation of Insoluble Fe³⁺, Al³⁺, Cu²⁺, and Ca²⁺ Complexes

Pusino

Micera

Gessa

1991

Clays and clay miner.

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Abstract--Analytical, spectroscopic, and X-ray powder diffraction techniques were used to investigate the adsorption of the herbicide sodium acifluorfen (acifluorfen = 5-[2-chloro-4-(trifluoromethyl)phenoxy]-2-nitrobenzoic acid) from aqueous solution onto Cu 2.-, AP +-, Fe3+-, and Ca2*-saturated montmorillonite. As indicated by infrared and electron spin resonance spectra, which are coincident with those of the corresponding solid metal complexes, and by the basal spacings, which are the same for all the samples, acifluorfen extracted the exchangeable ions from the clay interlayer and precipitated them on external surfaces. The process involved the replacement of sodium as the saturating ion and was due to the formation of insoluble complexes between the herbicide and polyvalent metal ions.

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“…After 24 hr the films were separated from the solution and washed with CHC13 to remove excess pesticide. After air-drying, the films were examined by infrared spectroscopy (IR), and the amount of released metal (Fe 3+, AI 3+ , and Cu 2+ ) was determined by analysis of the leachates using colorimetric methods (Bandemer and Schaible, 1944;Chenery, 1948;Nebesar, 1964).…”

Section: Adsorption Of Pesticidementioning

confidence: 99%

Interaction of Diclofop and Diclofop-Methyl with Al³⁺-, Fe³⁺-, and Cu²⁺-Saturated Montmorillonite

Pusino

Micera

Gessa

et al. 1989

Clays and clay miner.

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Abstract--The adsorption from chloroform solution of the herbicide diclofop, (RS)-2-(4-(2,4-dichlorophenoxy)phenoxy)propionic acid, and its methyl ester diclofop-methyl on Cu 2 § A13 § and Fe3+-exchanged bentonite samples was investigated. For comparison, the complexes formed by diclofop were synthesized and studied. Diclofop adsorbed on the clays and formed carboxylate bonds to the interlayer ions. Diclofop-methyl also adsorbed, but its interaction involved the formation of hydrogen bonds with water molecules in the interlayer. Traces of diclofop were observed in both the solution and the clays after adsorption of diclofop-methyl, indicating that some hydrolysis of the ester to the corresponding acid occurred. Thus, pesticides forming neutral complexes with interlayer cations in montmorillonite in soils may be extractable by solvents and therefore released into the environment.

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Spectrophotometric Determination of Copper in Tellurium and Related Thermoelectric Compounds of the Bismuth Telluride Type with 2,9-Dimethyl-1,10-phenanthroline.

Cited by 25 publications

References 13 publications

Histone deacetylase from HeLa cells: properties of the high molecular weight complex

Histone deacetylase from HeLa cells: properties of the high molecular weight complex

Interaction of the Herbicide Acifluorfen with Montmorillonite: Formation of Insoluble Fe³⁺, Al³⁺, Cu²⁺, and Ca²⁺ Complexes

Interaction of Diclofop and Diclofop-Methyl with Al³⁺-, Fe³⁺-, and Cu²⁺-Saturated Montmorillonite

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Spectrophotometric Determination of Copper in Tellurium and Related Thermoelectric Compounds of the Bismuth Telluride Type with 2,9-Dimethyl-1,10-phenanthroline.

Cited by 25 publications

References 13 publications

Histone deacetylase from HeLa cells: properties of the high molecular weight complex

Histone deacetylase from HeLa cells: properties of the high molecular weight complex

Interaction of the Herbicide Acifluorfen with Montmorillonite: Formation of Insoluble Fe3+, Al3+, Cu2+, and Ca2+ Complexes

Interaction of Diclofop and Diclofop-Methyl with Al3+-, Fe3+-, and Cu2+-Saturated Montmorillonite

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Product

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About

Interaction of the Herbicide Acifluorfen with Montmorillonite: Formation of Insoluble Fe³⁺, Al³⁺, Cu²⁺, and Ca²⁺ Complexes

Interaction of Diclofop and Diclofop-Methyl with Al³⁺-, Fe³⁺-, and Cu²⁺-Saturated Montmorillonite