Spectrophotometric determination of cyclotrimethylenetrinitramine (RDX) in explosive mixtures and residues with the Berthelot reaction

Üzer, Ayşem; Erçağ, Erol; Apak, Reşat

doi:10.1016/j.aca.2008.02.015

Cited by 27 publications

(5 citation statements)

References 36 publications

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“…The indophenol method has been widely used to quantify urea after splitting it enzymatically with urease [ 33 ] and other substrates [ 34 , 35 ], and used in determining of deaminase activity [ 36 ]. This method can be easily adjusted to detect other metabolites or substances using a suitable deaminase.…”

Section: Discussionmentioning

confidence: 99%

An Enzymatic Assay for High-Throughput Screening of Cytidine-Producing Microbial Strains

Dong

Liu

et al. 2015

PLoS ONE

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Cytidine is an industrially useful precursor for the production of antiviral compounds and a variety of industrial compounds. Interest in the microbial production of cytidine has grown recently and high-throughput screening of cytidine over-producers is an important approach in large-scale industrial production using microorganisms. An enzymatic assay for cytidine was developed combining cytidine deaminase (CDA) and indophenol method. CDA catalyzes the cleavage of cytidine to uridine and NH3, the latter of which can be accurately determined using the indophenol method. The assay was performed in 96-well plates and had a linear detection range of cytidine of 0.058 - 10 mM. This assay was used to determine the amount of cytidine in fermentation flasks and the results were compared with that of High Perfomance Liquid Chromatography (HPLC) method. The detection range of the CDA method is not as wide as that of the HPLC, furthermore the correlation factor of CDA method is not as high as that of HPLC. However, it was suitable for the detection of large numbers of crude samples and was applied to high-throughput screening for high cytidine-producing strains using 96-well deep-hole culture plates. This assay was proved to be simple, accurate, specific and suitable for cytidine detection and high-throughput screening of cytidine-producing strains in large numbers of samples (96 well or more).

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Section: Discussionmentioning

confidence: 99%

An Enzymatic Assay for High-Throughput Screening of Cytidine-Producing Microbial Strains

Dong

Liu

et al. 2015

PLoS ONE

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“…Mattos et al [8] used the FT-IR (Fourier-transform infrared spectroscopy) method in the near (NIR) and mid (MIR) IR regions, and measured RDX in HMX and compared the results by high performance liquid chromatography (HPLC) method. Uzer et al [9] were able to measure the RDX in HMX using the spectrophotometer in the visible region. The detection limit of the method was 0.18 g ml −1 .…”

Section: Introductionmentioning

confidence: 99%

Statistical optimization for determination of trace amounts of RDX in matrix of HMX using GC-ECD

2019

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1,3,5,7-Tetranitro-1,3,5,7-tetrazocane (HMX) is one of the most powerful military explosives that is produced by means of Bechmann method. Simultaneous, 1,3,5-rinitroperhydro-1,3,5-triazine (RDX) as a by-product is produced in this method that affect on the purity and sensitivity of HMX. Using a simple and fast technique is important for determination of trace amounts of RDX in the HMX matrix. The technique of gas chromatography-electron capture detector (GC-ECD) was proposed for these reasons. Because the presence of several parameters in the proposed technique, the experimental design methods of factorial and central composite were used for the experiments of screening and optimization, respectively. The parameters of oven programming, injection volume, injection temperature, carrier gas flow rate, and detector temperature were used in experiments of screening. The analysis of variance and Pareto plots showed that the injection temperature and carrier gas flow rate must be optimized among the parameters. After the screening and optimization of the studied parameters, the selected conditions were oven programming 10 °C min −1 , injection volume 1 μL, injection temperature 250 °C, carrier gas (N 2 ) flow rate 3 mL min −1 , and detector temperature 300 °C. The calibration curve and detection limit of the optimized method were 10-90 and 1.8 mg L −1 , respectively, of RDX in matrix of HMX. The relative standard deviation of the measurement was 1.89%. Finally, the RDX recovery percentages in HMX matrix in the three spiked samples were also calculated.

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“…Picric acid, one of the phenols, will do massive damages to human eyes, skin and respiratory systems [9]. The detection of picric acid is of great importance because a large amount of industries such as organic synthesis and drugs analysis all inevitably involve picric acid [10], which imposes severe risks on human health and the environment.As a consequence,many efforts have been devoted to the development of efficient technologies for the determination of picric acid in the environment.Several analytical methods have been developed for determining picric acid such as spectrophotometric [11], fluorescence [12,13], mass spectrometry [14,15], high-performance liquid chromatography [16], extraction [17], capillary electrophoresis [18], and electrochemical methods [19][20][21][22]. Compared to the above-mentioned methods, electrochemical techniques have advantages such as simplicity, selectivity, rapidity, low cost and easy miniaturization [23,24].…”

Section: Introductionmentioning

confidence: 99%

Quantification of picric acid on nanosphere polypyrrole modified electrode by stripping voltammetric method

et al. 2019

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The electrochemical studies of picric acid were carried out in acidic, neutral and basic buffer media at bare glassy carbon (GC) and polypyrrole modified GC electrode. Cyclic Voltammogram (CV) of picric acid exhibited three reduction peaks at -0.4, -0.8 and -1.5V (vs. Ag/AgCl) and two oxidation peaks at 0.8 and 1.4V (vs. Ag/AgCl). Among the various pH studied, highly sensitive response was observed at pH 1.0. The effect of scan rate was studied between 25 and 500 mVs-1 at the optimal pH.CV results revealed the adsorption-controlled reaction at the electrode surface. The GC electrode was modified with polypyrrole conducting polymer film to enhance the electrocatalytic activity of the reductive species. Atomic force microscopy (AFM) images showed the nanosphere morphology of the polypyrrole film, which was coated uniformly on the electrode surface. Under optimum experimental conditions, the influence of concentration on the stripping signal was studied. The linear range of detection was found between 50 ppb and 250 ppb with the lower limit of detection of 10±3 ppb.

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Spectrophotometric determination of cyclotrimethylenetrinitramine (RDX) in explosive mixtures and residues with the Berthelot reaction

Cited by 27 publications

References 36 publications

An Enzymatic Assay for High-Throughput Screening of Cytidine-Producing Microbial Strains

An Enzymatic Assay for High-Throughput Screening of Cytidine-Producing Microbial Strains

Statistical optimization for determination of trace amounts of RDX in matrix of HMX using GC-ECD

Quantification of picric acid on nanosphere polypyrrole modified electrode by stripping voltammetric method

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