Spectrophotometric determination of eflornithine hydrochloride using vanillin as derivative chromogenic reagent

Kumar, Amit; Singh, Vijender; Kumar, Praveen

doi:10.4314/tjpr.v13i11.21

Cited by 9 publications

(8 citation statements)

References 8 publications

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“… method reagent linear range LOQ LOD sample ref. (μg ml −1 ) photometric dansyl chloride 0.5–4.5 0.052 0.017 bulk [ 17 ] photometric vanillin 5–25 4.3 1.2 vials [ 18 ] photometric 1,2-naphthoquinone-4-sulfonate 10–70 0.52 0.172 bulk [ 19 ] photometric para -dimethylamino benzaldehyde 5–40 3.94 1.184 vials [ 20 ] proposed acetylacetone/formaldehyde 0.1–0.8 0.06 0.02 cream, urine and plasma …”

Section: Resultsmentioning

confidence: 99%

“…Therefore, the estimation of EFN in various biological media has a particular value for investigating pharmacodynamics and pharmacokinetics as well as monitoring the absorption and tissue uptake of EFN in both pre-clinical and clinical research [11]. A survey of the literature explained that there are several analytical chromatographic methods such as LC-evaporative light scattering detector [12], LC-UV detector [13][14][15], LC-MS/MS detector [16] and few spectrophotometric methods (UV and colorimetric) [17][18][19][20] have been reported to estimate EFN. In addition, one enzyme-linked immunosorbent assay method [21] and one electrochemical method also have been reported [11].…”

Section: Introductionmentioning

confidence: 99%

“…However, the expensive cost, the time loss, ultrafiltration, the harmful organic solvents consumption and the sophisticated laboratory set-up are known to be the major drawbacks of the abovementioned liquid chromatographic methods [12][13][14][15][16]. In addition, the reported spectrophotometric methods are limited to analyse the cited drug in vials dosage only without broaching to cream samples or the biological fluid analysis [17][18][19][20]. Hence, a cost-effective and uncomplicated analytical method with sufficient levels of sensitivity and selectivity is required for the quantitation of EFN in biological fluids and pharmaceutical cream.…”

Section: Introductionmentioning

confidence: 99%

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Application of Hantzsch reaction for sensitive determination of eflornithine in cream, plasma and urine samples

Almahri

Abdel‐Lateef

2021

R. Soc. open sci.

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Eflornithine (EFN) is an anti- Trypanosoma brucei agent for the medication of sleeping sickness and widely distributed for the treatment of hirsutism (unwanted facial hair in women). The presented work demonstrates a comprehensive analytical approach for the spectrofluorometric determination of EFN in commercial cream samples and various biological samples. The proposed method is based on the formation of a highly yellow–green fluorescence dihydropyridine derivative after the interaction between EFN and acetylacetone/formaldehyde reagent in a slightly acidic medium. Furthermore, the optimal variables such as reagent volumes, pH of the medium, heating time, buffer volume, heating temperature and diluting solvent were carefully selected to achieve the maximum fluorescence activity. The fluorescence activity for the formed derivative was measured at λ emission = 477 nm after λ excitation = 418 nm. Concerning linearity, accuracy, sensitivity, precision and robustness, the presented method was validated and verified according to ICH guidelines. Moreover, the proposed work offered a selective determination for EFN in various brands of pharmaceutical cream without any interference from excipients. Eventually, the current approach was assured to be successful in the estimation of EFN in urine and plasma samples with acceptable recovery results.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Application of Hantzsch reaction for sensitive determination of eflornithine in cream, plasma and urine samples

Almahri

Abdel‐Lateef

2021

R. Soc. open sci.

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“…The HPLC techniques currently available for the quantification of DFMO in biological fluids involve either pre or post column derivatization with UV or fluorescence detection [9,10] and LC carried out by evaporative light scattering detection [11]. Few methods have been reported in the literature for the analysis of DFMO including spectrophotometry [12,13].…”

Section: Introductionmentioning

confidence: 99%

Determination and Validation of Eflornithine Hydrochloride by (Rp-Hplc) Reversed Phase High Performance Liquid Chromatography

Kumar¹,

Singh²,

Kumar³

2013

AJPS

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A simple highly sensitive, rapid, precise, accurate and specific RP-HPLC method was developed for quantification of eflornithine hydrochloride (2-difluoromethyl-DL-ornithine; DFMO) in its commercial formulation. In this RP-HPLC method, the separation was performed using BDS Hypersil 5µ C18 (150 X 4.6 mm) column at room temperature by using methanol: 2% of Glacial acetic acid in water (80:20 v/v) as mobile phase. The flow rate was 0.8 mL min -1 with UV detection at 290 nm. The retention time of DFMO was 4.3. Linearity was observed over concentration range of 50-100 µg mL -1 for DFMO. The LOD and LOQ were found to be 0.008438 µg mL -1 and 0.028126 µg mL -1 respectively. The accuracy of the proposed method was determined by recovery studies and found to be 100.5 % for DFMO. The proposed method was validated for ICH guidelines like linearity, limit of detection, accuracy, precision, ruggedness, robustness, and system suitability.

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“…The HPLC techniques currently available for the quantification of DFMO in biological fluids involve either pre or post column derivatization with UV or fluorescence detection [13,14] and LC carried out by evaporative light scattering detection [15]. Few methods have been reported in the literature for the analysis of DFMO including spectrophotometry [16,17].…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of Newer High Performance Thin Layer Chromatographic Method for Quantification of Eflornithine Hydrochloride in Pharmaceutical Formulations

Kumar¹,

Singh²,

Kumar³

2013

AJPS

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A new, simple, sensitive, precise and robust high performance thin layer chromatography (HPTLC) method was developed for the estimation of eflornithine hydrochloride (DFMO) in pharmaceutical dosage forms. Estimation was performed on TLC aluminum plates precoated with silica gel 60F 254 as stationary phase. Linear ascending development was carried out in twin trough glass chamber saturated with mobile phase consisting of methanol: chloroform: acetic acid: 1% triethylamine (4:6:0.1:0.5 v/v/v) at room temperature (25±2°C). Analysis of the plate in absorbance mode at 220nm was carried out. The system was found to give compact spots for DFMO with R f (Retardation factor) value of 0.55 respectively. The data for calibration plots showed good linear relationship with correlation coefficient of 0.999 in the concentration range of 300-800ng mL -1 for DFMO respectively. The values of limit of detection (LOD) were 0.6238ng mL -1 and limit of quantification (LOQ) were 1.8903ng mL -1 for DFMO respectively. The accuracy of the method was 100.44%. The precision demonstrated a relative standard deviation of less than 2.5%. The results were satisfactory when compared with the literature. This new method was validated according to the International Conference on Harmonization (ICH) guidelines which include linearity, precision, accuracy, specificity, robustness, detection and quantitation limits. The developed methods found to be sufficiently precise and reproducible for established conditions and after validation may be used for routine analysis of eflornithine hydrochloride in pharmaceutical formulations.

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Spectrophotometric determination of eflornithine hydrochloride using vanillin as derivative chromogenic reagent

Cited by 9 publications

References 8 publications

Application of Hantzsch reaction for sensitive determination of eflornithine in cream, plasma and urine samples

Application of Hantzsch reaction for sensitive determination of eflornithine in cream, plasma and urine samples

Determination and Validation of Eflornithine Hydrochloride by (Rp-Hplc) Reversed Phase High Performance Liquid Chromatography

Development and Validation of Newer High Performance Thin Layer Chromatographic Method for Quantification of Eflornithine Hydrochloride in Pharmaceutical Formulations

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