Spectrophotometric determination of isopropamide Iodide and trifluoperazine hydrochloride in presence of trifluoperazine oxidative degradate

Abstract

Four sensitive, selective and precise stability indicating methods for the determination of isopropamide iodide (ISO) and trifluoperazine hydrochloride (TPZ) in their binary mixture and in presence of trifluoperazine oxidative degradate (OXD). Method A is a derivative spectrophotometric one, where ISO was determined by first derivative (D(1)) at 226.4 nm while TPZ was determined by second derivative (D(2)) at 270.2 nm. Method B is the first derivative of the ratio spectra (DD(1)) spectrophotometric method, ISO can be determined by measuring the peak amplitude at 227.4 nm using 5 microg mL(-1) of OXD as a divisor, while TPZ can be determined by measuring the peak amplitude at 249.2 and 261.4 nm using 15 microg mL(-1) of ISO as a divisor. Method C is the isoabsorptive spectrophotometric method. This method allows determination of ISO and TPZ in their binary mixture by measuring total concentration of ISO and TPZ at their isoabsorptive point at lambda(229.8) nm (Aiso1) while TPZ concentration alone can be determined at lambda(max) 311.2 nm, then ISO concentration can be determined by subtraction. On the same basis TPZ can be determined in presence of ISO and OXD, where OXD concentration alone was determined by measuring the peak amplitude at lambda(281.6) and lambda(309.4) nm while total concentration of TPZ and OXD was determined at their isoabsorptive points at (Aiso2 = 270.2 nm), (Aiso3 = 310.6 nm) and (Aiso4 = 331.8 nm) then TPZ concentration was determined by subtraction. Method D is the multivariate calibration techniques [the classical least squares (CLS), principal component regression (PCR) and partial least squares (PLS)], using the information contained in the absorption spectra of ISO, TPZ and OXD mixtures. The selectivity of the proposed methods was checked using laboratory prepared mixtures. The proposed methods have been successfully applied to the analysis of ISO and TPZ in pharmaceutical dosage form without interference from other dosage form additives and the results were statistically compared with the reported method.

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“…[12] There are only few spectrophotometric methods for estimation trifluoperazine individually. [13][14][15][16] A few other analytical methods have been reported with trifluoperazine and isopropamide simultaneously, individually and with other drugs.…”

Spectrophotometric Determination of Isopropamide Iodide based on Ion-pair Complex Formation with Thymol Blue

“…Simultaneous spectrophotometric estimation of ISO in the existence of trifluoperazine as a tranquilizer and its oxidative degradation was reported as well 19 . Frequently, trifluoperazine comes in a dualistic mixture with ISO for their anti-vomiting and anti-convulsive influence 20 . Similarly, derivative spectrometries were reported also since they have the privilege of simultaneous analysis of multi-component systems.…”

Comparative Application of PLS and PCR Methods to Simultaneous Quantitative Estimation and Simultaneous Dissolution Test of Zidovudine - Lamivudine Tablets

“…Particularly, PLS and PCR approaches are preferable calibration techniques mainly because they provide an efficient quantitative resolution of complex mixtures of drugs. [21][22][23][24][25][26][27][28][29][30][31][32][33] In the work presented in this study, two different chemometric calibration approaches, PLS and PCR were applied for the quantification and dissolution testing of ZID and LAM in commercial tablets. Both PLS and PCR approaches don't require any prior separation step such as extraction and precipitation etc.…”