Spectrophotometric Determination of L-Ascorbic Acid in Pharmaceuticals Based on Its Oxidation by Potassium Peroxymonosulfate and Hydrogen Peroxide

Abstract

Abstract. Two new, accurate, precise, and sensitive spectrophotometric methods were developed for the assay of L-ascorbic acid in pharmaceutical preparations. The determination of L-ascorbic acid was based on its oxidation by potassium peroxymonosulfate (method A) and hydrogen peroxide in the presence of Cu(II) as a catalyst (method B). The molar absorptivities were found to be 1.48×10 4 and 1.06×10 4 L mol -1 cm -1 for methods A and B, respectively. Beer's law was obeyed in the concentration range of 0.65-11.20 μg mL -1 for method A and 0.51-16.00 μg mL -1 for method B. Other compounds commonly found in vitamin C and multivitamin products did not interfere with the determination of L-ascorbic acid.. The proposed methods were successfully applied for the determination of L-ascorbic acid in pharmaceutical formulations. The results obtained with the proposed methods showed good agreement with those given by the titrimetric method using iodine.

Simultaneous determination of rutin and ascorbic acid mixture in their pure forms and combined dosage form

“…Different methods have been developed for the individual determination of ascorbic acid [9][10][11][12][13][14][15][16][17]and rutin [18][19][20][21][22]. In addition, Several methods have been reported for simultaneous determination of mixture of these active ingredients like UV spectrophotometry [23,24], electrochemical method [25][26][27], chemiluminescence [28], capillary electrophoresis [29][30][31][32], high-performance liquid chromatography (HPLC) [33][34][35][36]and NIR FTIR [37].…”

Spectrofluorometric method for the determination of ascorbic acid in pharmaceutical preparation using l‐tyrosine as fluorescence probe

“…[ 20 ] Chelating agents, such as ethylenediaminetetraacetic acid (EDTA), could be used as stabilizers to mask any interference caused by Cu 2+ ions. [ 21 ] After addition of EDTA, ascorbic acid in the presence of Cu 2+ ions remained stable for at least 2 h and recovery increased to 95.08%. Therefore, the proposed method displayed good specificity.…”

Electrochemical Sensor Based on a Carbon Veil Modified by Phytosynthesized Gold Nanoparticles for Determination of Ascorbic Acid

“…A variety of analytical methods are used to determine AA: titrimetric [2,3], spectrophotometric [2][3][4], chromatographic [5][6][7][8], fluorimetric [9], chemiluminescent and electrochemical methods [10][11][12]. Traditional methods of redox titration do not allow a quantitative assessment of the content of ascorbic acid in stained samples.…”