Spectrophotometric determination of methylamine moiety containing pesticides using ninhydrine reagent

Abstract

A spectrophotometric method has been proposed for the determination of pesticides containing methylamine moiety. These pesticides afforded methylamine upon alkaline hydrolysis, which was subse quently reacted with ninhydrin in the presence of pyridine. The absorbance of the resulting blue purple prod uct was measured spectrophotometrically at 570 nm. The Beer's law showed a linear relationship between absorbance and concentration of the pesticide in the range 0.04-5.0 µg/mL. The limit of detection and limit of quantification were 0.012 and 0.02 µg/mL, respectively. The repeatability was 0.22 expressed as RSD. The performance of this newly developed method was compared with the established spectrophotometric meth ods for the determination of these pesticides. The method has been successfully applied to the evaluation of these pesticides in commercial pesticide formulation and to the determination of monocrotophos in fruit samples.

Ninhydrin in synthesis of heterocyclic compounds

“…[39][40][41][42][43][44][45] Ninhydrin is extensively used to detect compounds of pharmaceutical importance [46][47][48][49][50][51][52][53][54][55][56][57] and in kinetic studies. [58][59] Scheme 1.…”