1950
DOI: 10.1021/ac60039a013
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Spectrophotometric Determination of Nicotine

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1952
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Cited by 114 publications
(36 citation statements)
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“…On completion of smoking, the trap was allowed to attain room temperature, condensed smoke was washed from the trap with acetone (about 300 ml. ), the washings filtered through glass wool and an aliquot taken to check non-volatile whole smoke yield by determination of nicotine by the method of Willits, Swain and Connelly (1950) Application of materials to the skin Condensates were applied by means of a foot operated automatic pipette, delivering a volume of 0*3 ml. to an area of dorsal skin 1-5 cm.…”
Section: Smoking Proceduresmentioning
confidence: 99%
“…On completion of smoking, the trap was allowed to attain room temperature, condensed smoke was washed from the trap with acetone (about 300 ml. ), the washings filtered through glass wool and an aliquot taken to check non-volatile whole smoke yield by determination of nicotine by the method of Willits, Swain and Connelly (1950) Application of materials to the skin Condensates were applied by means of a foot operated automatic pipette, delivering a volume of 0*3 ml. to an area of dorsal skin 1-5 cm.…”
Section: Smoking Proceduresmentioning
confidence: 99%
“…Determination of nicotine content was conducted according to Willits et al (1950). The total protein content was determined by a total protein quantitative assay kit (Nanjing Jiancheng Bioengineering Institute).…”
Section: Methodsmentioning
confidence: 99%
“…Nicotine determination.-Nicotine content of condensates were determined by the method of Willits et al (1950), as modified by Laurene and Harrell (1958).…”
Section: Methodsmentioning
confidence: 99%