Spectrophotometric Determination of Oxymetazoline Hydrochloride Via Oxidative Coupling Reaction with 4-Aminoantipyrine in the Presence of Potassium Periodate

Abstract

A simple spectrophotometric method is developed for the determination of oxymetazoline hydrochloride (OMCl) as pure and in its pharmaceutical preparations. The method is based on the oxidative coupling reaction of OMCl with 4-aminoantipyrine (4-A.A.P) in the presence of potassium periodate as oxidizing agent in an alkaline medium to produce a coloured water soluble product that is stable and has a maximum absorption at 480 nm. Beer's law is obeyed in a concentration range 20-400µg OMCl/20 ml with a molar absorptivity of 5.34 x 10 3 1.mol-1 .cm-1 , a relative error of-0.50 to-1.47% and a relative standard deviation of ± 0.36 to ± 1.58%, depending on the concentration level. The optimum conditions for full colour development are described and the proposed method was applied successfully to the assay of OMCl in two pharmaceutical preparations.

Spectrophotometric method for Determination of oxymetazoline, HCl in a Pharmaceutical formulation using 2,4 - dinitrophenylhydrazine

“…The formed green color product gives the highest absorption at pH 11.2, therefore; the effect of the amount of buffer solution of sodium hydrogen phosphate -sodium hydroxide solution has been studied using increased volumes of the buffer solution ranging from 0.5 -3.0 ml (23) . The results are shown in Table (4). The results shown in Table (4) indicate that the addition of different volumes of the buffer solution leads to a decrease in the absorption of colored product, so it is avoided in subsequent experiments.…”

“…(2) Different analytical methods have been used for the determination of OXMZ, such as spectrophotometric methods (3)(4)(5)(6)(7) , high performance liquid chromatographic methods HPLC (8)(9)(10)(11)(12)(13)(14)(15)(16) , electrochemical method (17)(18)(19)(20) and flow-injection techniques (21)(22) . In this research a rapid, sensitive and simple spectrophotometric method for determining of OXMZ in pure form as well as in pharmaceutical drops formulations based on the oxidative coupling using 2,4-dinitrophenylhydrazine (2,4-DNPH) in presence of potassium periodate in basic medium.…”

Development HPLC technique for determining Oxymetazoline and Isoxspurine in pharmaceutical formulations

“…Various analytical methods have been used to determine isoxoprene hydrochloride, including high performance liquid chromatography [6][7][8], fluorescence [9], flow injection [10,11], spectrophotometry [12][13][14][15][16][17], gas chromatography [18] and for the determination of oxymetazoline hydrochloride, including HPLC [19][20][21][22], spectrophotometry [23][24][25][26][27][28][29], flow injection chemistry Luminescence [30], Ion Selective Electrode [31][32][33][34][35][36] and Fluorescence Spectrophotometry[37] methods.…”

Utilization of Localized Surface Plasmon Resonance of Silver Nanoparticles for the Spectrofluorimetric Estimation of Oxymetazoline in Dosage Forms: Application to Aqueous Humor

“…Oxymetazoline is found in several nasal spray decongestants and ophthalmic drops. It directly stimulates α receptors on blood vessels supplying the nasal mucosa and conjunctiva, thereby producing vasoconstriction and decreasing congestion [8].It is absorbed from systemic circulation regardless of the route of administration [9] .OMZ is o cial in each of the United States Pharmacopoeia(USP) [10], the British Pharmacopoeia (BP) [11], and the European Pharmacopoeia [12].Reviewing the literature revealed few reports for OMZ determination ,viz, spectrophotometry [13][14][15][16][17][18], spectro uorimetry, , [19,20] gas chromatography [21,22], HPLC [10][23-28], ow injectionchemiluminescence [29,30],capillary electrophoresis [31], and electrochemistry [32,33].…”