Spectrophotometric Determination of Risedronate Sodium in Pharmaceutical Preparations by Derivative and Continuous Wavelet Transforms

Abstract

Determination of risedronate sodium (RIS) in its commercial tablet formulations in the presence of the effect of excipients was performed by derivative spectrophotometry and continuous wavelet transform. No preliminary separation step was used for the quantitative analysis by the proposed methods. Firstly direct absorbance measurement (DAM) method was applied to the analysis of RIS in samples, but this method did not give desirable results for the analysis of the commercial tablet samples. For this reason, the signal processing methods, first derivative spectrophotometry (DS), Morlet and Biorthogonal2.8 continuous wavelet transforms (Morlet-CWT and Bior2.8-CWT, respectively) were subjected to the quantitative resolution of the samples containing RIS and tablet excipients without using any separation step. These methods were found to be suitable for the analysis of the related drug. Calibration graphs were obtained by using the relationships between first derivative, CWT signals and concentration. The linearity ranges of DS and CWT methods were found to be 0.8-120 μg/mL, 3.0-100.0 μg/mL respectively and good correlations were observed for the calibration equations. The proposed methods were validated and applied to the determination of RIS in pharmaceutical preparations. The experimental results obtained by the proposed methods were statistically compared with each other and with those obtained by spectrophotometric method reported in literature. In the statistical analysis, no significant difference was found between the assay results. It was observed that the DS, Morlet-CWT and Bior2.8-CWT methods were accurate, sensitive, precise, rugged and useful for the quality control of RIS in commercial pharmaceutical samples without the interference of the excipients.

Pharmaceuticals and Related Drugs

“…Many of the experimental procedures involve direct measurements of the analyte after relatively simple sample preparations steps, usually involving an extraction for cleanup, and others are based on more elaborate sample handling and chemical derivatization steps. A few examples of the many spectrophotometric assays appear in Table − . In the selection of these citations, particular emphasis was placed on chemiluminesence and fluorescence approaches and in selecting a cross-section of different types of compounds.…”

“…Although it is difficult to discuss spectrophotometric methods in terms of more general trends, it does appear that specrofluorimetric approaches are gaining in popularity in contrast to more conventional UV−visible spectrophotometry due to greater specificity and increased sensitivity. Some of the procedures are based on native fluorescence measurements ,,,,,,,,, or employ a surfactant to enhance the emission intensity ,,,, . In the case of the latter procedures, sodium dodecylsulfate and sodium dodecylbenzene sulfonate were the most commonly used additives.…”