Spectrophotometric determination of sperm concentration and short-term cold-storage of sperm in Atlantic croaker<i>Micropogonias undulatus</i>L. broodstock

Leclercq, Éric; Antoni, Luca; Bardon‐Albaret, Agnès; Anderson, Corey R; Somerset, Carly R.; Saillant, Eric

doi:10.1111/are.12073

Cited by 8 publications

(8 citation statements)

References 35 publications

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“…Compared with other Sciaenids, the results obtained for meagre sperm motility over storage time are similar or better. Atlantic croaker semen in a 1:2 or 1:9 dilution in HBSS (Hank's Balanced Salt Solution) retained motility for less than 4 days (Leclercq et al., ), whereas in the present work, an average of 38% and 31% motile cells were observed in the 1:4 or 1:9 dilutions in 0.9% NaCl at 4 days of storage respectively. Semen of black drum, Pogonias cromis L., diluted in 202 or 286 mOsm/kg HBSS, retained 8% motile cells at the eighth day of cold storage (Wayman, Thomas, & Tiersch, ), whereas in this experiment, semen diluted 1:4 in 0.9% NaCl retained 13% motility until the ninth day.…”

Section: Discussionmentioning

confidence: 42%

Evaluation of different extenders for the cold storage of meagre (Argyrosomus regius ) semen

et al. 2018

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Meagre (Argyrosomus regius) is considered a potential candidate for aquaculture diversification in southern Europe. The main objective of this experiment was to develop a cold storage protocol for meagre semen to facilitate artificial reproduction techniques. Three extenders (non-activating medium, 0.9% NaCl, and 0.9% NaCl with glycine and glucose) in three different sperm:extender dilutions (1:4, 1:9 and 1:19) were tested in a full factorial design. The quality parameters assessed along the storage time were the sperm motility, viable sperm percentage, adenosine triphosphate (ATP) content, and bacterial growth. The 0.9% NaCl and 0.9% NaCl with glycine and glucose extenders and the 1:4 and 1:9 dilutions maintained a higher sperm motility and a higher sperm linearity for a longer period time.Sperm viability was maintained at a higher value over a longer period with the 0.9% NaCl and 0.9% NaCl with glycine and glucose extenders. Sperm motility and viability appeared to be the main parameters showing the loss of semen quality during cold storage. Meagre semen demonstrated an ability to be stored for up to 10 days at 4°C when using 0.9% NaCl in a 1:4 dilution. These results contribute to a better understanding of the causes of fish semen quality deterioration during cold storage.

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Section: Discussionmentioning

confidence: 42%

Evaluation of different extenders for the cold storage of meagre (Argyrosomus regius ) semen

et al. 2018

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“…The wavelength generating the best fit regression model differs in among species. For example, 400 nm was used in Atlantic croaker and small bodied fishes (Tan, Yang & Tiersch ; Leclercq, Antoni, Bardon‐Albaret, Anderson, Somerset & Saillant ), 320 in blue mussels (Del Rio Portilla & Beaumont ), 581 nm in Pacific oysters (Dong et al . ) and 350 nm in the Australian flat oysters (present study).…”

Section: Discussionmentioning

confidence: 99%

Development of a spectrophotometric technique for sperm quantification in the spermcasting Australian flat oyster Ostrea angasi Sowerby

Hassan

Qin

2017

Aquac Res

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Sperm quantification is vitally important when sperm concentration is required for standardization of different fertilization treatments in a hatchery. Although the haemocytometer method is generally used to determine sperm concentration, the procedure is tedious and the attributes are not suitable for handling a large number of sperm samples within a short period. In this study, the efficiency of sperm concentration determination was improved in the spermcasting oyster Ostrea angasi Sowerby by optimizing the regression model and parameters critical to spectrophotometric reading. Although sperm concentration can be estimated in a wide range of wavelengths, the 350-nm wavelength produced the best fit to the regression model (y = 1 9 10 À8 x + 0.163; r 2 = 0.996). In addition, the sperm counts estimated with this model were similar to the haemocytometer counts. The reading repeatability of this technique was further validated with samples from different individuals. Comparisons with literature suggest that when the spectrophotometric technique is applied to a new species for estimating sperm concentration, the regression relationship between sperm concentration and wavelength reading should be reassessed due to speciesspecific discrepancy.

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“…The last method (d) assesses the linear relationship between particle concentration and light absorption (17) . It requires an initial calibration with reading by a hemocytometer and provides accurate correlation indices with sperm concentration (18) .…”

Section: Introductionmentioning

confidence: 99%

Spectrophotometric method for determination of Colossoma macropomum (tambaqui) sperm concentration

et al. 2023

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To assist the reproductive management of tambaqui (Colossoma macropomum) males in laboratory and commercial fish farming, a linear regression model was obtained from concentration curves using the spectrophotometric method. Twenty-two tambaqui males with an average age of three years old were selected and divided into two groups containing 11 animals each. Both groups alternately received a single dose of carp pituitary extract (CPE; 2.0 mg/kg body weight, intracoelomic). Sperm was collected 14 h after hormonal treatment and diluted (1:4000; sperm:formaldehyde saline). The concentration was estimated by counting spermatozoa in a Neubauer chamber and by using a spectrophotometer (λ=540 nm). Individual sperm concentration ranged from 11.40 to 71.13 × 109 sperm/mL. The degree of transmittance ranged from 62.1% to 95.0%. There was a significant correlation (r2 = 0.966; p < 0.0001) between sperm concentration analyzed in a Neubauer chamber and transmittance at 540 nm. Analysis by spectrophotometry and the prediction provided by the equation Y=100.293 - 0.509X proved to be an efficient and fast method for estimating sperm concentration in tambaqui and can be used in routine procedures in artificial fish reproduction laboratories.

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Spectrophotometric determination of sperm concentration and short-term cold-storage of sperm in Atlantic croakerMicropogonias undulatusL. broodstock

Cited by 8 publications

References 35 publications

Evaluation of different extenders for the cold storage of meagre (Argyrosomus regius ) semen

Evaluation of different extenders for the cold storage of meagre (Argyrosomus regius ) semen

Development of a spectrophotometric technique for sperm quantification in the spermcasting Australian flat oyster Ostrea angasi Sowerby

Spectrophotometric method for determination of Colossoma macropomum (tambaqui) sperm concentration

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