Spectrophotometric Determination of Tetracycline Hydrochloride in Pure Form and Pharmaceutical Preparation by Coupling with Diazotized Anthranilic Acid

Abstract

A simple, fast and sensitive spectrophotometric method has been applied for the determination of tetracycline hydrochloride in its pure form and in pharmaceutical preparations. The method based on coupling reaction of the antibiotic with diazotized anthranilic acid to form a stable yellow azo dye which shows a maximum absorption at 419 nm. Uni- and multivariate approaches were followed in optimizing the experimental conditions. Under optimum experimental conditions obtained via multivariate (Central Composite Design), the linearity of the constructed calibration curve was in the range of 0.560 μg.mL-1 with molar absorptivity of 14619 L.mol-1.cm-1 and the value of detection limit was 0.2813μg.mL-1. The capability of the method for the analysis of real tetracycline samples was evaluated. The results show that the method could successfully be applied with no significant interference from the common excipients.

Quality of Amoxicillin Trihydrate/Potassium Clavulanate Tablets Commercially Available in Libyan Pharmaceutical Market

“…There are many and various analytical methods previously reported for assay of co-clavimox tablets which includes thin layer chromatography (TLC) [19][20][21][22], ultraviolet (UV) spectrophotometry [23,24], titration [25], high-performance liquid chromatography (HPLC) [26][27][28][29], capillary electrophoresis (CE) [30], and infrared spectroscopy (IR) [31], in both British Pharmacopoeia (B. P) and United States Pharmacopoeia (U. S. P). Among then, HPLC is recommended for the analysis of co-clavimoxin pure forms and pharmaceutical preparations.…”

Spectrophotometric assay of oxymetazoline-HCl in pure form and in its pharmaceutical formulations using potassium permanganate.pdf

“…Several spectrophotometric methods have also been employed to the assay of OXM in bulk and its pharmaceutical preparations used various reagents for determining OXM, these reagents included: 2,6-dichloroquinone chlorimide with an oxidant reagent (Sankar et al, 1987), potassium ferricyanide -Fe(III) (Othman and Sahar, 2013), 4-chloro-7-nitrobenzo-2-oxa-1, 3-diazole (Salama et al, 2018), 2,4,6-tris(2-pyridyl)-5-triazine (Sankar et al, 1988), 2,4-dinitrophenylhydrazine (Humeidy, 2015), 1,10phenanthroline -iron(III) (Al-Sabha and Rasheed, 2011), 4-aminoantipyrine (Zakaria, 2011), cobaltinitrite-Na (Shingbal and Naik, 1983), cerium sulphate (IV) (Mohammed and Ikram, 2016) and 2,2-bipyridyl (Basheer and Mohammed, 2018). Some of these methods suffer from various limitations, for example, low sensitivity, and a long time to complete the reaction, and low stability of the product.…”

Spectrophotometric determination of amoxicillin in pharmaceutical formulations using normal and reverse flow injection analysis systems: A comparison study

“…Despite these advantages, concerns regarding AMX residues in food and the possible environmental contamination risk associated with this antibiotic have been raised due to its side effects on human health [4]. The literature covered a variety of analytical techniques [5] for the extraction and determination of AMX, including spectrofluorometry [6,7], electrochemistry [8][9][10], liquid chromatography-mass spectroscopy [11][12][13], highperformance thin layer chromatography (HPTLC) [14], high pressurized liquid chromatography (HPLC) [15], FIA [16][17][18][19][20], magnetic molecularly imprinted polymer [21], and spectrophotometry [22][23][24]. Flow-injection analysis (FIA) techniques are frequently utilized and have generated great interest because of their reproducibility, simplicity, and low apparatus costs [25][26][27][28][29].…”