Spectrophotometric determination of tetracyclines in pharmaceutical preparations, with uranyl acetate

Saha, Utpal; Sen, Ashim Kumar; Das, Tarun; Bhowal, S. K.

doi:10.1016/0039-9140(90)80192-i

Cited by 49 publications

(21 citation statements)

References 2 publications

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“…They suppress the reproduction of Gram-positive and Gramnegative microorganisms, acid-resistant bacilli, Rickettsia and many viruses [1][2][3]. Owing to such a broad spectrum of action, they are widely used to combat infections and human disease and also for veterinary therapy [4][5][6]. Several methods have been reported for their determinations, both in pharmaceutical formulations and biological samples (fluids and tissues) including methods such as spectrophotometric [7][8][9][10], amperometric [11,12], chemiluminescence [13,14], spectrofluorimetric [15,16] and liquid chromatographic [17][18][19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

A simple spectrophotometric method for the determination of tetracycline and doxycycline in pharmaceutical formulations using chloramine-T

et al. 2010

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A simple, rapid, accurate and inexpensive spectrophotometric method for the determination of tetracycline and doxycycline has been developed. The method is based on the reaction between these drugs and chloramine-T in alkaline medium producing red color products with absorbance maximum at the ë = 535 and 525 nm for the tetracycline and doxycycline, respectively. The best conditions for the reactions have been found using mol L and 7.12 x 10 mol L for the tetracycline and doxycycline, respectively. The proposed method was successfully applied to the determination of these drugs in pharmaceutical formulations and the results obtained were in good agreement with those obtained by the comparative method at the 95% confidence level.

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Section: Introductionmentioning

confidence: 99%

A simple spectrophotometric method for the determination of tetracycline and doxycycline in pharmaceutical formulations using chloramine-T

et al. 2010

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“…For the determination of methacycline hydrochloride, high performance liquid chromatography [8] and antibiotics-microbial assay [9] were recommended in some pharmacopoeias. Only few studies on the method for determination of methacycline(MC) in pharmaceutical analysis are reported, including high performance liquid chromatography [10], spectrophotometry [11], phosphorimetry [12] and thin-layer chromatography [13][14]. Ion-selective electrodes are playing an important role in pharmaceutical analysis [15][16][17] due to its simplicity, rapidity and accuracy over some other analytical methods.…”

Section: Introductionmentioning

confidence: 99%

Ion Selective PVC Membrane Electrode for the Determination of Methacycline Hydrochloride in Pharmaceutical Formulation

Aboul‐Enein

Sun

2002

Sensors

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A plastic membrane electrode for the determination of methacycline hydrochloride was fabricated based on the use of methacycline-tetraphenylborate as the electroactive substance, and dioctylphtalate as the plasticizing agent. It was found that the potential responses of the electrode were influenced by pH of the tested solution when pH>3. Under the condition of pH 2.6, the linear response range, slope (25ºC) and detection limit obtained were 3.0×10 -2~6.0×10-6 M, 52.9mv per decade and 3.4×10 -6 M ,respectively.The response time of the electrodes was <15 seconds. The values of recovery obtained were 99.2~101.2%. The electrode was successfully applied to determination of methacycline hydrochloride in tablet by direct potentiometric method. The result obtained with the electrode was in good agreement with the value obtained by using the official method (highperformance liquid chromatography).

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“…A few visible spectrophotometric methods based on the different reaction schemes are found in the literature for the assay of DOX. These include FIA-spectrophotometry with copper carbonate (5) and spectrophotometry based on colour reactions with thorium(IV) (6), sodium cobaltnitrite (7) and uranyl acetate (8). Besides, kinetic spectrophotometry using Cu(II)/H 2 O 2 (9) and multivariate calibration method (10) have also been reported by different authors.…”

mentioning

confidence: 99%

“…Although these procedures are specific, most of the described methods are time consuming and require multistage extraction procedures. On the other hand, the reported spectrophotometric methods (5)(6)(7)(8)(9)(10) are for different reasons not satisfactory for the routine quality assurance. Some of these methods suffer from disadvantages such as poor sensitivity, low stability of the species, use of organic solvent, scrupulous control of experimental variables and special equipment (Table VII).…”

mentioning

confidence: 99%

Sensitive and selective spectrophotometric assay of doxycycline hyclate in pharmaceuticals using Folin-Ciocalteu reagent

Ramesh¹,

Basavaiah²,

Rajendraprasad³

2010

Acta Pharmaceutica

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Doxycycline hyclate (DOX), chemically known as (4S,4aR,5S,5aR,6R,12aS)-4-(dimethylamino)-3,5,10,12,12a-pentahydroxy-6-methyl-1,11-dioxo-1,4,4a,5,5a,6,11,12a-octahydrotetracene-2-carboxamide hydrochloride hemiethanol hemihydrate (Fig. 1), is one of the tetracycline derivatives, which has a wide range of antibacterial activities. DOX is frequently used to treat chronic prostatitis, sinusitis, syphilis, chlamydia, pelvic inflammatory disease, acne, rosacea, and rickettsial infections.The drug is official in British Pharmacopoeia (1), which describes the HPLC method for the determination of DOX either in raw material or in pharmaceutical formulations. A spectrophotometric method for the determination of doxycycline (DOX) is described. The method is based on the formation of blue colored chromogen due to reduction of tungstate and/or molybdate in Folin-Ciocalteu (F-C) reagent by DOX in alkaline medium. The colored species has an absorption maximum at 770 nm and the system obeys Beer's law over the concentration range 0.75-12.0 mg mL -1 DOX. The apparent molar absorptivity is 2.78´10 4 L mol -1 cm -1 . The limit of quantification and detection values are reported to be 0.20 and 0.08 mg mL -1 , respectively. Over the linear range applicable, the accuracy and precision of the method were evaluated on intra-day and inter-day basis. The reported mean accuracy value was 101.0 ± 1.7 %, the relative error was £ 2.7 % and the relative standard deviation was £ 2.5 %. Application of the proposed method to bulk powder and commercial pharmaceutical tablets is also presented. No significant difference was obtained between the results of the proposed method and the official BP method. The procedure described in this paper is simple, rapid, accurate and precise.

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Spectrophotometric determination of tetracyclines in pharmaceutical preparations, with uranyl acetate

Cited by 49 publications

References 2 publications

A simple spectrophotometric method for the determination of tetracycline and doxycycline in pharmaceutical formulations using chloramine-T

A simple spectrophotometric method for the determination of tetracycline and doxycycline in pharmaceutical formulations using chloramine-T

Ion Selective PVC Membrane Electrode for the Determination of Methacycline Hydrochloride in Pharmaceutical Formulation

Sensitive and selective spectrophotometric assay of doxycycline hyclate in pharmaceuticals using Folin-Ciocalteu reagent

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