1976
DOI: 10.1080/00015127609435074
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Spectrophotometric Determination of Total and Free Cholesterol in Egg Yolk and Animal Tissues

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Cited by 20 publications
(6 citation statements)
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“…At the end of the experiment, 20 eggs from each group were collected to estimate the egg quality parameters (yolk weight and relative weight to total egg weight, yolk index, albumin weight, and its relative weight, albumen index, shell thickness and weight as well as its relative weight were recorded) according to Card and Nesheim (17). Six samples of egg yolk from each group were collected for egg yolk cholesterol determination according to Rotenberg and Christensen (18).…”
Section: Egg Production and Egg Qualitymentioning
confidence: 99%
“…At the end of the experiment, 20 eggs from each group were collected to estimate the egg quality parameters (yolk weight and relative weight to total egg weight, yolk index, albumin weight, and its relative weight, albumen index, shell thickness and weight as well as its relative weight were recorded) according to Card and Nesheim (17). Six samples of egg yolk from each group were collected for egg yolk cholesterol determination according to Rotenberg and Christensen (18).…”
Section: Egg Production and Egg Qualitymentioning
confidence: 99%
“…The extracted lipids were recovered in small tubes. The egg yolk cholesterol was determined in the filtered sample by spectrophotometric method described by Rotenberg and Christensen [20] using chemistry analyzer (Map Lab Plus, Japan) at 546 nm.…”
Section: Cholesterol Analysismentioning
confidence: 99%
“…The total lipid of the egg yolk was extracted using the chloroform: methanol (2:1) method as explained by Folch et al, 1957. Yolk cholesterol concentration was determined according to Rotenberg and Christensen (1976) using cholesterol reagent and standard (Spinreact, Citra Coloma, Spain). FA profile of the egg yolk was analyzed according to Radwan (1978) using gas chromatograph (Hewlett Packard, 6890).…”
Section: Chemical Analyses Of the Eggs Yolkmentioning
confidence: 99%