Spectrophotometric determination of traces of nitrite with rhodamine 6 G

Kartikeyan, S.; Rao, T. Prasada; Iyer, C.S.P.; Damodaran, A. D.

doi:10.1007/bf01245306

Cited by 7 publications

(5 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…), 0.2 ml of 12.5 M H 2 SO 4 , and 1.1 ml of water, and the optical density at 525 mm was measured. This nitrite quantification method has been shown to be very comparable to more established methods when assaying foods for nitrite content (16) and is assumed to be reliable for cleared soil extracts as well.…”

Section: Methodsmentioning

confidence: 97%

See 1 more Smart Citation

Impact of trichloroethylene and toluene on nitrogen cycling in soil

Fuller

Scow

1997

Appl Environ Microbiol

View full text Add to dashboard Cite

The effects of trichloroethylene (TCE) and toluene on soil nitrogen-cycling activities were examined. Ammonium oxidation potential (AOP) was reduced after incubation with as little as 1 g of TCE ml ؊1 , and the effects were generally greater when toluene was present and increased with longer exposure. Arginine ammonification potential and denitrification enzyme activity were constant regardless of TCE concentration or the presence of toluene, while nitrite oxidation potential (NOP) exhibited variable sensitivity. KCl-extractable ammonium levels increased dramatically after exposure to 30 and 60 g of TCE ml ؊1 in the presence of toluene, whereas gamma-irradiated or sodium azide-treated soil incubated with the same concentrations of TCE and toluene showed no increase. Alfalfa-amended soils showed similar decreases in AOP and increases in extractable ammonium during incubation with 60 g of TCE ml ؊1 and 20 g of toluene ml ؊1 , although most probable number estimates of the ammonium oxidizer population showed no difference between exposed and unexposed soil. AOP and extractable ammonium returned slowly to control levels after 28 days of incubation in the presence of TCE and toluene. Activity assays to which various TCE and toluene concentrations were added indicated that AOP and NOP were relatively more sensitive to these compounds than was arginine ammonification potential. These results indicate that the soil microbial populations responsible for nitrogen cycling exhibit different sensitivities to TCE and toluene and that they may be more susceptible to adverse effects than previously thought.

show abstract

Section: Methodsmentioning

confidence: 97%

“…Nitrite concentrations were determined by the spectrophotometric method of Kartikeyan et al (16). Two-milliliter samples from the AOP or NOP assays were centrifuged at 10,000 ϫ g for 5 min to pellet soil particles.…”

Section: Methodsmentioning

confidence: 99%

Impact of trichloroethylene and toluene on nitrogen cycling in soil

Fuller

Scow

1997

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…This method is based on diazo coupling between sulfanilic acid and N-(1-naphthyl)ethylenediamine dihydrochloride. Methods based on reactions other than diazo coupling have also been developed [11][12][13]. Methods were also reported involving the extraction of the azo dye formed, to improve the detection limit [14][15][16].…”

mentioning

confidence: 99%

Extractive spectrophotometric determination of trace amounts of nitrogen dioxide, nitrite, and nitrate

Malingappa

Balasubramanian

1996

Mikrochim Acta

View full text Add to dashboard Cite

Abstract.A sensitive extractive spectrophotometric method for the determination of nitrogen dioxide in air and nitrite and nitrate in water, soil and blood serum is described. Nitrogen dioxide in air is fixed as nitrite in a suitable trapping solution. The method is based on the diazo coupling reaction between pnitroaniline and N-(1-naphthyl)ethylenediamine dihydrochloride [NEDA]. The azo dye formed under aqueous conditions is extracted with isobutyl methyl ketone [IBMK]. The system obeys Beer's law over the range 0-3 Ixg of nitrite at 545 nm and the colour is stable for 3 h. The molar absorptivity of the colour system is 5.7 x 10 4 L mol-lcm-1. The relative standard deviation is 1.3% for ten determinations at 2 gg of nitrite. Nitrate is determined as nitrite after reduction on a cadmium column. Negative interferences from SO2, HzS , Cu 2 + and Cr a § and positive interference from Fe 2 § and Fe 3 § can be simply masked.

show abstract

“…9 The retention efficiency was found to be better than 98% with a sample throughput of 60/h. The developed method has been successfully applied to seawater samples.…”

Section: ·1 Fia-faas 2·1·1 Preconcentration By the Formation Of Metamentioning

confidence: 95%