Spectrophotometric Determination of Zinc in Pharmaceutical Medication Samples Using 8-Hydroxyquinoline Reagent

Abstract

Simple, rapid, cheap and sensitive spectrophotometric method has been described for the determination of zinc in pharmaceutical samples. The method is based on the formation of zinc- 8-Hydroxy quinoline chelate, the maximum absorption (lmax) at 384 nm. The method obeyed Beer's law in the range 1-5 mg/mL and the corresponding molar absorptivity value is 0.01578 ´ 103 L.mol-1.cm-1. The Sandell sensitivity values of limits of detection (LOD) and quantification (LOQ) was 0.381mg/mL and 1.156 mg/mL respectively. The recovery percentage of zinc was found 98.00 %, 98.96 %,99.91%, 97.50%, 98.5% and 99.30 % for (Capsule-13 mg), (Tablet-20 mg), (Tablet-40 mg),(Capsule-50 mg), (Capsule-50 mg) and (insulin vial-0.025 mg) respectively. All variable parameters has been optimized according to ICH guidelines. The limiting concentrations of some cations for interference by Mn(II), Fe(II), Co(II), Ni(II), Cu(II), Cd(II),Sn(II) ,Pb(II), Mg(II), Ca(II) and Ba(II) are reported. The method accuracy was established by comparison with conventional flame atomic absorption spectrometric method by using t-test, ttab.= 2.571, tcal. =0.3231 at 95% confidence level, indicating the absence of systematic errors.

Spectrophotometric Determination of Micro Amount of Chromium (III) Using Sodium 4-((4,5-diphenyl-imidazol-2-yl)diazenyl)-3-hydroxynaphthalene-1-sulfonate in the Presence of Surfactant, Study of Thermodynamic Functions and Their Analytical Applications

“…Azo compounds with the formula (R-N=N-R) are known as Azo fibrous groups and are not widely used in various fields because they are unstable due to their rapid decomposition into nitrogen, whereas Azo compounds with the formula (Ar-N=N-Ar) are known as aromatic Azo compounds and are more stable due to their active donor (N=N) [2][3][4][5] . When heterocyclic Azo compounds combine with metal ions, they form chelates coordination complexes that are useful in analytical and biological chemistry [6][7][8][9] . Azo compounds have potent pharmacological activity, and when administered as a metal complex, the drug becomes exceedingly potent 10 .…”

Quantitative Determination of Lincomycin in Dosage Forms by Iodometric Titration through Oxidation Reaction with Peroxomonosulfate

“…atomic absorption spectroscopy [8], as well as other types of spectroscopy [9][10][11][12], chromatography [13][14][15][16][17][18][19][20][21], Lateral Flow…”