Spectrophotometric measurement of carotenes, xanthophylls, and chlorophylls in extracts from plant seeds

Bulda, O. V.; Rassadina, V. V.; Alekseichuk, H. N.; Laman, N. A.

doi:10.1134/s1021443708040171

Cited by 43 publications

(35 citation statements)

References 19 publications

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“…Along with phenolics they are responsible for bright colors. This chemical class acts as antioxidants, with functions that include protection of membranes against damage by free radicals and retardation of ageing processes [20]. Hydrolysis did not cause any observable change in total carotenoid compared with the control (Table 3).…”

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confidence: 96%

Evaluation of Bioactive Components and Antioxidant and Anticancer Properties of Citrus Wastes Generated during Bioethanol Production

Kim

2014

Natural Product Communications

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In the bioethanol production process employing citrus peels, a large amount of enzymatic hydrolyzed residues is generated as waste material. The bioactive compounds, and antioxidant and anticancer activities of these residues were investigated in the present study. Hydrolyzed citrus residues exhibited similar antioxidant activity as the unhydrolyzed control, which was positively correlated to the contents of total phenols, flavonoids and total carotenoid. Some flavonoids (naringin, naringenin, hesperetin and neohesperidin) and two high value co-products (D-limonene and galacturonic acid) were detected only in hydrolyzed residues. In addition, hydrolyzed residues showed antiproliferative activity and sub-G1 arrest in human melanoma A375 and colon cancer HCT116 cells. These results provide an alternative use for hydrolyzed citrus residues in the functional food, cosmetic and pharmaceutical industries.

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confidence: 96%

Evaluation of Bioactive Components and Antioxidant and Anticancer Properties of Citrus Wastes Generated during Bioethanol Production

Kim

2014

Natural Product Communications

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“…However, rather than aiming to identify and quantify individual caretenoids present, the current study simply aimed to quantify changes in leaf Chl content in response to drought stress. In such cases, spectrophotometric pigment assays such as the one used here (after Arnon 1949) are sufficient (Bulda et al 2008), but stability of the pigment extract should be checked because extraction with acetone solutions can lead to Chl degradation to phaeophytin by co‐extracted acids (Rodrigues‐Amaya and Kimura 2004). This was unlikely to have affected the results obtained here because the absorbance of acetone Chl extracts of A. belladonna leaves remained stable (to the third decimal) for as long as 6 h after filtration (when kept in the dark).…”

Section: Methodsmentioning

confidence: 99%

Effects of cryopreservation of recalcitrant Amaryllis belladonna zygotic embryos on vigor of recovered seedlings: a case of stress ‘hangover’?

Berjak

Pammenter

2010

Physiologia Plantarum

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Cryopreservation is the most promising long-term storage option for recalcitrant (i.e. desiccation-sensitive) seed germplasm; however, its effects on the vigor of recovered seedlings are unclear. This study looked at the vigor of seedlings recovered from partially dried (D) and cryopreserved (C) recalcitrant zygotic embryos (ZEs) of Amaryllis belladonna. Seedlings recovered from fresh (F), D- and C-embryos were regenerated in vitro, hardened-off ex vitro and then exposed to 12 days of watering (W) or 8 days of water deficit (S), followed by 3 days of re-watering. Seedling vigor was assessed in terms of physiological and growth responses to the imposed water stress. Compared with F-embryos, partial dehydration and cryopreservation reduced the number of embryos that produced seedlings, as well as the subsequent in vitro biomass of these seedlings. DW- and CW-seedlings (i.e. seedlings recovered from dried and cryopreserved ZEs that were watered for 12 days) exhibited lower CO(2)-assimilation rates and abnormal root growth. Stomatal density was also lower in C-seedlings. DS- and CS-seedlings were exposed to persistent low leaf water and pressure potentials and unlike FS-seedlings, displayed signs of having incurred damage to their photosynthetic machinery. CS-seedlings were less efficient at adjusting leaf water potential to meet transpirational demands and more susceptible to persistent turgor loss than DS- and FS-seedlings. DS-seedlings performed slightly better than CS-seedlings but drought-induced seedling mortality in both these treatments was higher than FS-seedlings. These results suggest that seedlings recovered from partially dried and cryopreserved embryos were less vigorous and more susceptible to hydraulic failure than those from fresh ZEs.

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“…When analyzing the absorption spectra, two absorption bands were noted in the blue-violet region of 380 − 500 nm and in the red region of 640 − 670 nm. Peaks at 420 (1) and 664 nm (5) correspond to chlorophyll a [12]. Peaks of 453, 649 nm correspond to chlorophyll b, shoulder (4) at 420 nm is described for protochlorophyll [14].…”

Section: Cultivation Of C Sorokiniana In Various Light Conditionsmentioning

confidence: 99%

Directed cultivation of Chlorella sorokiniana for the increase in carotenoids’ synthesis

et al. 2020

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The metabolism of Chlorella sorokiniana is subjected to changes caused by various cultivation conditions. If dosed ultraviolet radiation is used, there is a possibility of a compensatory increase in the synthesis of carotenoids which prevent oxidative stress. Strain 211-8k was cultured under various lighting conditions: control sample was subjected to fluorescent light; sample 1 was subjected to dosed periodic ultraviolet irradiation for 15 minutes every day and fluorescent lighting; sample 2 was subjected to ultraviolet irradiation for 30 minutes in the stabilization phase. Periodic UV exposure negatively affects the population growth of C. sorokiniana which was possible to detect only on the ninth day and the biomass yield significantly decreased. A single UV exposure for 30 minutes lead to a slight decrease in the yield of air-dry biomass which with a further population growth may be compensated. Periodic exposure to UV radiation stimulates carotenoids synthesis, the yield in terms of dry biomass exceeded the control sample on average by 30%. A single ultraviolet irradiation for 30 minutes in the stabilization phase lead to a decrease in the biomass content of both chlorophyll and carotenoids. Microscopy of microalgae showed that ultraviolet radiation leads to the formation of cells with signs of apoptosis.

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Spectrophotometric measurement of carotenes, xanthophylls, and chlorophylls in extracts from plant seeds

Cited by 43 publications

References 19 publications

Evaluation of Bioactive Components and Antioxidant and Anticancer Properties of Citrus Wastes Generated during Bioethanol Production

Evaluation of Bioactive Components and Antioxidant and Anticancer Properties of Citrus Wastes Generated during Bioethanol Production

Effects of cryopreservation of recalcitrant Amaryllis belladonna zygotic embryos on vigor of recovered seedlings: a case of stress ‘hangover’?

Directed cultivation of Chlorella sorokiniana for the increase in carotenoids’ synthesis

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