Spectrophotometric Method for the Determination Amikacin in Pure and Pharmaceutical Dosage Form

Abstract

Objective: The aim of the study was to develop an easy, sensible and rapid method for the estimation of amikacin in both pure and marketed formulation using the spectrophotometric method.Methods: Due to lack of chromophoric group in the amikacin, it was derivatized with 0.1 mmol chloranillic acid reagent. For the estimation of amikacin, Shimadzu UV-1700 model spectrophotometer with UV probe software was used. The method was based on simple charge transfer complexation of the drug with a p-chloranillic acid reagent to give a purple coloured product which was measured at 524nm against blank solution. Results:The derivatised product of amikacin was detected at a wavelength of 524 nm. Linearity was observed with the concentration range of 20-100 µg/ml with a regression coefficient of 0.9803. Results of all the parameters were within the acceptance criteria with % RSD less than 2. Conclusion:The spectroscopic method was validated as per ICH guidelines and was found to be applicable for routine quantitative analysis of amikacin in marketed formulations also. The results of linearity, precision, accuracy LOD and LOQ were within the specified limits. The method is highly sensitive, robust, reproducible and specific.

Comparative Study between Herbal and Synthetic Antidepressant Drugs

“…Administration of ethanolic extract of A. aspera to the animals at a dose of 200 mg/kg and 400 mg/kg significantly decrease in the duration of estrous & metesterous phase. Treated the animals with ethanolic extract of it at a dose of 200 and 400 mg/kg reduced the weight of ovaries than the control group [18].…”

“…The result showed that the ethanolic extract of A. aspera having antifertility activity. It also possesses the antizygotic, blastocytotoxic and antiovulatory activities [18]. Antiarthritic activity of A. aspera has been carried out by using most popular method, known as inhibition of protein denaturation.…”