Spectrophotometric Methods for Quantitative Determination of Chlorhexidine Gluconate and its Major Impurity, Metabolite and Degradation Product: Para-chloro-aniline

Abdelrahman, Maha M.; Naguib, Ibrahim A.; Elsayed, Mohamed A.; Zaazaa, Hala E.

doi:10.1080/22297928.2016.1196148

Cited by 12 publications

(5 citation statements)

References 10 publications

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“…After dissolving the polymeric patch, the solution was centrifuged at 3,000 rpm for 10 minutes. The absorbance of the supernatant solution was recorded at 251 nm using a UV spectrophotometer to determine the content of chlorhexidine (Abdelrahman et al, 2016;Fini et al, 2011). To estimate the concentration of chlorhexidine, a calibration was constructed at a concentration range of 0-20 µg/ ml.…”

Section: Phase 2: Determination Of Drug Content and In-vitro Drug Release Studiesmentioning

confidence: 99%

“…The calibration curve was linear with an R 2 value of 0.9605 and the corresponding equation was y = 0.0218x + 0.053. The in-vitro release of chlorhexidine from the polymeric patches was carried out by using a vial method at room temperature for 20 days (Amirthalingam et al, 2017;Shen and Burgess, 2012). The polymeric patch (area: 25 mm 2 ) from each batch was kept in the glass vials containing 1 ml of phosphate buffer pH 6.8.…”

Section: Phase 2: Determination Of Drug Content and In-vitro Drug Release Studiesmentioning

confidence: 99%

See 1 more Smart Citation

Intraoral slow-releasing polymeric patches containing sodium fluoride and chlorhexidine: Development and evaluation

Karuna

Nayak²,

Pralhad³

et al. 2020

J App Pharm Sci

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Early childhood caries (ECC) has been considered to be at epidemic proportions in developing countries. There is a scientific consensus regarding the benefit of a constant supply of low levels of fluoride, especially at the biofilm/saliva/ tooth interface in preventing dental caries. The use of controlled and sustained delivery systems containing fluoride can be considered as an effective means of eradicating ECC in high-risk individuals. The use of chlorhexidine as an antimicrobial agent in combination with fluoride is beneficial (Naidu et al., 2016). Slow-releasing devices containing both fluoride and chlorhexidine do not exist as of now. Hence, the aim of the present study was to design a slow-release polymeric intraoral patch containing fluoride and chlorhexidine, and to evaluate the amount of medicament released over 20 days. Three slow-releasing patches were fabricated with different concentrations of gelatin to determine the maximum release of medicaments. Using in-vitro drug release studies it was observed that, out of three patches prepared with equal concentrations of chlorhexidine (80 mg) and sodium fluoride (288 mg), a patch I containing 1,000 mg of gelatin was found to be beneficial for a sustained release of both medicaments.

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Section: Phase 2: Determination Of Drug Content and In-vitro Drug Release Studiesmentioning

confidence: 99%

Section: Phase 2: Determination Of Drug Content and In-vitro Drug Release Studiesmentioning

confidence: 99%

Intraoral slow-releasing polymeric patches containing sodium fluoride and chlorhexidine: Development and evaluation

Karuna

Nayak²,

Pralhad³

et al. 2020

J App Pharm Sci

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“…The evaluation is based on the SD of the response and the slope of the curves of the four studied pesticides, but using a visual non instrumental method regarding to ICH recommendations [15][16][17][18][19][20][21][22][23] for HPTLC method (Table 1).…”

Section: Limits Of Detection and Quantizationmentioning

confidence: 99%

Two chromatographic methods for the quantitative determination of some pesticides applied for cucumber pests in Egypt

Abdelfatah

Hegazy

Mahmoud

et al. 2018

Separation Science Plus

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Two novel sensitive and accurate thin‐layer chromatography and high‐performance liquid chromatography methods were used for monitoring the residues of four pesticides: mandipropamid, metalaxyl, thiophenate methyl and carbendazim, which are widely used to eliminate cucumber pests in Egypt. The first method is a thin‐layer chromatography method, using silica gel plates as a stationary phase and hexane/methanol/acetone/glacial acetic acid (7.5:2:0.5:0.1, v/v) as a developing system. The second method is high‐performance liquid chromatography using isocratic elution and a mobile phase consisting of acetonitrile/0.02 molar potassium dihydrogen phosphate buffer (25:75, v/v) at a flow rate of 1 mL min−1 with UV detection at 225 nm. All the obtained results were evaluated regarding the agricultural committee of Egypt and showed acceptable pesticides residues.

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“…In modern analytical laboratories, there is a need for significant stability in analysis techniques, and therefore, analytical methods are continuously changing and developing. Over the past dozen years, numerous papers describing these methods have appeared, such as reversed-phase high-performance liquid chromatography [10][11][12], liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) [13][14][15], spectrophotometric methods [16,17], and electrochemical methods [18][19][20]. The Polish Pharmacopoeia [21] recommends titration in anhydrous acetic acid medium with perchloric acid at 0.1 mol L −1 .…”

Section: Introductionmentioning

confidence: 99%

A Novel Polymeric Membrane Sensor for Chlorhexidine Determination

Lenik,

Sokal

2023

Sensors

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In the present work, potentiometric sensors with polymer membranes used for chlorhexidine (CHXD) determination were developed. The polymer membranes were plasticized with bis(2-ethylheksyl)sebacate (DOS) or 2-nitrophenyloctyl ether (o-NPOE). The active compounds used in the membrane were cyclodextrins, crown ethers, and ion exchangers. The best-constructed electrode was based on neutral heptakis(2,3,6-tri-O-benzoyl)-β-cyclodextrin with lipophilic salt (KTpClBP)—potassium tetrakis(4-chlorophenyl) borate—dissolved in plasticizer, DOS. The presented electrode is characterized by an average cationic slope of 30.9 ± 2.9 mV decade−1 within a linear range of 1 × 10−6 to 1 × 10−3 mol × L−1, while the value of the correlation coefficient is 0.9970 ± 0.0026. The response time was about 5 s when increasing the sample concentration and about 10 s when diluting the sample. The electrode potential is independent of the pH within a range of 4.0–9.5. The polymeric membrane sensor was successfully applied for assays of chlorhexidine digluconate in pure samples and pharmaceutical samples. The relative error from three replicate measurements was determined to be 1.1%. and the accuracy was RSD = 0.3–1.1%.

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Spectrophotometric Methods for Quantitative Determination of Chlorhexidine Gluconate and its Major Impurity, Metabolite and Degradation Product: Para-chloro-aniline

Cited by 12 publications

References 10 publications

Intraoral slow-releasing polymeric patches containing sodium fluoride and chlorhexidine: Development and evaluation

Intraoral slow-releasing polymeric patches containing sodium fluoride and chlorhexidine: Development and evaluation

Two chromatographic methods for the quantitative determination of some pesticides applied for cucumber pests in Egypt

A Novel Polymeric Membrane Sensor for Chlorhexidine Determination

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