Spectrophotometric quantification of lactose in solution with a peroxidase-based enzymatic cascade reaction system

Fornera, Sara; Yazawa, Kenjiro; Walde, Peter

doi:10.1007/s00216-011-5312-9

Cited by 26 publications

(13 citation statements)

References 21 publications

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“…44,45 Analysis of diluted milk samples by SALDI-MS on weathering steel sample plates produced a clean spectrum for lactose and isobaric galactose/glucose in the case of lactase treated lactosefree milk (Fig. Particularly lactose is an important constituent of cow milk and, as a response to the widespread inherited intolerance against lactose among consumers, the dairy industry offers low-lactose and lactose free milk and derived products.…”

Section: Lactose Quantication In Milkmentioning

confidence: 99%

Nanostructured weathering steel for matrix-free laser desorption ionisation mass spectrometry and imaging of metabolites, drugs and complex glycans

Etxebarría

Calvo

Reichardt

2014

Analyst

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Weathering steel has been employed for the first time to prepare sample plates for matrix-free laser desorption ionisation mass spectrometry (LDI-MS) of small molecules up to a mass range of around 1500 Da. The effective UV absorption, heat conductivity and porosity of the nanostructured inner rust layer formed during passivation determine the excellent performance in LDI-MS for a broad range of different analyte classes. The inexpensive material was evaluated in a series of relevant analytical applications ranging from the matrix-free detection of serum metabolites, lactose quantification, lipid analysis in milk to the glycoprofiling of antibodies and imaging mass spectrometry of brain tissue samples.

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Section: Lactose Quantication In Milkmentioning

confidence: 99%

Nanostructured weathering steel for matrix-free laser desorption ionisation mass spectrometry and imaging of metabolites, drugs and complex glycans

Etxebarría

Calvo

Reichardt

2014

Analyst

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show abstract

“…Thep roduction of glucose and galactose was then monitored by an external enzyme cascade reaction using an enzyme cocktail composed of glucose oxidase,horseradish peroxidase,and acolorimetric indicator, pyrogallol (Supporting Information, Figure S6). [46] Accordingly,a bsorbance at 420 nm was monitored as it is the result of the catalytic conversion of pyrogallol. Thecontinuous increase in intensity (Supporting Information, Figure S7) demonstrated that 1) lactose was able to diffuse through the ZIF-8 coating, 2) the MOF-coated b-gal was acting as ab iocatalyst for the production of essential sugars for the yeast cells,a nd 3) the biocatalyzed glucose diffused through the ZIF-8 shell.…”

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confidence: 99%

An Enzyme‐Coated Metal–Organic Framework Shell for Synthetically Adaptive Cell Survival

et al. 2017

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Abioactive synthetic porous shell was engineered to enable cells to survive in an oligotrophic environment. Eukaryotic cells (yeast) were firstly coated with a b-galactosidase (b-gal), before crystallization of ametal-organic framework (MOF) film on the enzyme coating;t hereby producing ab ioactive porous synthetic shell. The b-gal was an essential component of the bioactive shell as it generated nutrients (that is,glucose and galactose) required for cell viability in nutrientdeficient media (lactose-based). Additionally,the porous MOF coating carried out other vital functions,s uch as 1) shielding the cells from cytotoxic compounds and radiation, 2) protecting the non-native enzymes (b-gal in this instance) from degradation and internalization, and 3) allowing for the diffusion of molecules essential for the survival of the cells. Indeed, this bioactive porous shell enabled the survival of cells in simulated extreme oligotrophic environments for more than 7days, leading to ad ecrease in cell viability less than 30 %, versus a9 9% decrease for naked yeast. When returned to optimal growth conditions the bioactive porous exoskeleton could be removed and the cells regained full growth immediately.T he construction of bioactive coatings represents ac onceptually new and promising approach for the next-generation of cell-based researchand application, and is an alternative to synthetic biology or genetic modification.

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“…The production of glucose and galactose was then monitored by an external enzyme cascade reaction using an enzyme cocktail composed of glucose oxidase, horseradish peroxidase, and a colorimetric indicator, pyrogallol ( Figure S6, Supporting Information). [46] Accordingly, absorbance at 420 nm was monitored as it is the result of the catalytic conversion of pyrogallol. The continuous increase in intensity ( Figure S7, Supporting Information) demonstrated that 1) lactose was able to diffuse through the ZIF-8 coating; 2) the MOF coated β-gal was acting as a biocatalyst for the production of essential sugars for the yeast cells; 3) the biocatalyzed glucose diffused through the ZIF-8 shell.…”

mentioning

confidence: 99%

An Enzyme‐Coated Metal–Organic Framework Shell for Synthetically Adaptive Cell Survival

et al. 2017

View full text Add to dashboard Cite

A bioactive synthetic porous shell was engineered to enable cells to survive in an oligotrophic environment. Eukaryotic cells (yeast) were firstly coated with a β-galactosidase (β-gal), then a metalorganic framework (MOF) film was crystallized on the enzyme coating producing a bioactive porous synthetic shell. The β-gal was an essential component of the bioactive shell as it generated nutrients (i.e. glucose and galactose) required for cell viability from nutrient deficient media (lactose-based). In addition the porous MOF coating carried out other vital functions, such as: 1) shielding the cells from cytotoxic compounds and radiation, 2) protecting the non-native enzymes (β-gal in this instance) from degradation and internalization, 3) allowing for the diffusion of molecules essential for the survival of the cells. Indeed, this bioactive porous shell enabled the survival of cells in simulated extreme oligotrophic environments for more than 7 days, leading to a decrease in cell viability less than 30%, versus a 99% decrease for naked yeast. When returned to optimal growth conditions and the bioactive porous exoskeleton could be removed and the cells regained full growth immediately. Alternative to synthetic biology or genetic modification, the construction of bioactive coatings represents a conceptually new and promising approach for the nextgeneration of cell-based research and application.Living organisms are sensitive to their environment and subtle changes in nutrient level, temperature, pressure, moisture, salinity, and pH can disrupt their biological functions, thereby leading to cell death. [1][2][3] To address this challenge, primary

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Spectrophotometric quantification of lactose in solution with a peroxidase-based enzymatic cascade reaction system

Cited by 26 publications

References 21 publications

Nanostructured weathering steel for matrix-free laser desorption ionisation mass spectrometry and imaging of metabolites, drugs and complex glycans

Nanostructured weathering steel for matrix-free laser desorption ionisation mass spectrometry and imaging of metabolites, drugs and complex glycans

An Enzyme‐Coated Metal–Organic Framework Shell for Synthetically Adaptive Cell Survival

An Enzyme‐Coated Metal–Organic Framework Shell for Synthetically Adaptive Cell Survival

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