SPECTROSCOPIC ANALYSIS OF RHAMNOLIPID PRODUCED BY PRODUCED BY Pseudomonas aeruginosa UKMP14T

Sabturani, Noramiza; Latif, Jalifah; Radiman, Shahidan; Hamzah, Ainon

doi:10.17576/mjas-2016-2001-04

Cited by 21 publications

(12 citation statements)

References 25 publications

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“…The presence of a hydrophobic carbon source (chicken fats) favored the dominance of Rha(s)1 over Rha(s)2 as revealed by ESI-MS analysis shown in Section 2.2.1. Our results match those previously reported [42,48,49] where Rha(s)1 were the dominant compounds produced when the bacteria were grown in a medium supplemented with hydrophobic carbon sources, whereas using hydrophilic carbon sources favored the dominance of Rha(s)2.…”

Section: Discussionsupporting

confidence: 92%

Rhamnolipids Nano-Micelles as a Potential Hand Sanitizer

et al. 2021

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COVID-19 is a pandemic disease caused by the SARS-CoV-2, which continues to cause global health and economic problems since emerging in China in late 2019. Until now, there are no standard antiviral treatments. Thus, several strategies were adopted to minimize virus transmission, such as social distancing, face covering protection and hand hygiene. Rhamnolipids are glycolipids produced formally by Pseudomonas aeruginosa and as biosurfactants, they were shown to have broad antimicrobial activity. In this study, we investigated the antimicrobial activity of rhamnolipids against selected multidrug resistant bacteria and SARS-CoV-2. Rhamnolipids were produced by growing Pseudomonas aeruginosa strain LeS3 in a new medium formulated from chicken carcass soup. The isolated rhamnolipids were characterized for their molecular composition, formulated into nano-micelles, and the antibacterial activity of the nano-micelles was demonstrated in vitro against both Gram-negative and Gram-positive drug resistant bacteria. In silico studies docking rhamnolipids to structural and non-structural proteins of SARS-CoV-2 was also performed. We demonstrated the efficient and specific interaction of rhamnolipids with the active sites of these proteins. Additionally, the computational studies suggested that rhamnolipids have membrane permeability activity. Thus, the obtained results indicate that SARS-CoV-2 could be another target of rhamnolipids and could find utility in the fight against COVID-19, a future perspective to be considered.

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Section: Discussionsupporting

confidence: 92%

Rhamnolipids Nano-Micelles as a Potential Hand Sanitizer

et al. 2021

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“…LC-MS analysis in the present study revealed the abundance of six compositionally different homologues of mono-rhamnolipid congeners. However, a di-rhamnose congener, i.e., Rha-Rha-C 8 -C 10 or Rha-Rha-C 10 -C 8 was also obtained at m / z 621.5 with a retention time of 12.49 min ( Figure 2 b), which was also evident in the study by Sabturani et al [ 69 ]. Rhamnolipids are predominantly produced by Pseudomonas sp.…”

Section: Resultssupporting

confidence: 79%

Novel Bioformulations Developed from Pseudomonas putida BSP9 and Its Biosurfactant for Growth Promotion of Brassica juncea (L.)

Mishra

Fatima

Egamberdieva

et al. 2020

Plants

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In this study, Pseudomonas putida BSP9 isolated from rhizosphere of Brassica juncea was investigated for its plant growth promoting and biosurfactant producing activities. The isolate showed the ability to produce indole acetic acid, siderophore, phosphate solubilization activity and was an efficient producer of biosurfactant. Purification (of the biosurfactant) by thin layer chromatography (TLC) and further characterization by Fourier transform infrared spectroscopy (FTIR) revealed that biosurfactant produced by the isolate belonged to the glycolipid category, which is largely produced by Pseudomonas sp. In addition, liquid chromatography-mass spectroscopy (LC-MS) analysis showed the presence of a mixture of six mono-rhamnolipidic and a di-rhamnolipidic congeners, confirming it as a rhamnolipid biosurfactant. Bioformulations were developed using BSP9 and its biosurfactant to check their impact on promoting plant growth in B. juncea. It was noted from the study that bioformulations amended with biosurfactant (singly or in combination with BSP9) resulted in enhancement in the growth parameters of B. juncea as compared to untreated control. Maximum increment was achieved by plants inoculated with bioformulation that had BSP9 plus biosurfactant. The study also suggested that growth promotion was significant up to a threshold level of biosurfactant and that further increasing the concentration did not further enhance the growth parameter values of the plant. The study proves that novel bioformulations can be developed by integrating plant growth promoting rhizobacteria (PGPR) and their biosurfactant, and they can be effectively used for increasing agricultural productivity while minimizing our dependence on agrochemicals.

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“…The transfer process of product for storage and further use from the Schott bottle (used for precipitating the rhamnolipid) was a difficult task as the product was in the form of a sticky paste, which led to loss of product. The yield obtained in present study using this method is way higher than the yield obtained by Sabturani et al (2016) using solvent extraction in combination with acid precipitation (dual recovery method). Table 2 summarises a comparison between these recovery methods and in view of the results obtained in our study.…”

Section: Biosurfactant Recoverycontrasting

confidence: 53%

Extraction and antimicrobial activity of rhamnolipid biosurfactant produced by Pseudomonas aeruginosa UKMP14T

Firdose¹,

Msarah²,

Chong³

et al. 2021

MJM

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Aims: Rhamnolipids are seeking utmost attention as a new class of biosurfactants having promising potential in diverse fields as they offer a wide range of advantages over chemically synthesised surfactants. However, the high extraction costs make large scale production face difficulty. In present study, hydrocarbon degrading bacteria Pseudomonas aeruginosa UKMP14T was exploited for its biosurfactant producing ability including a comparative study between different extraction procedures for its recovery. In addition to this, the recovered biosurfactant was explored for its potential application as an antimicrobial agent. Methodology and results: The production of rhamnolipid biosurfactant was confirmed through various detection methods which are drop-collapse test, oil spreading assay, emulsification index, cetyltrimethylammonium bromide (CTAB) assay and hemolytic assay. The test strain P. aeruginosa UKMP14T showed positive results for all the detection assays. Following this, shake flask cultivation was carried out for several time intervals (1, 3, 5, 7 and 9 days) to discover the optimum time for rhamnolipid biosurfactant production. The results were evaluated by quantifying the rhamnolipid yield using Anthrone method and maximum yield was obtained on day 7. Then, three commonly employed rhamnolipid biosurfactant extraction methods (acid precipitation, solvent extraction and zinc sulphate precipitation) were incorporated for the extraction of rhamnolipid biosurfactant. Among these methods, organic solvent extraction (using methanol, chloroform and acetone in 1:1:1 ratio) gave the highest yield (7.37 ± 0.81 g/L) of biosurfactant, followed by zinc sulphate precipitation (5.83 ± 0.02 g/L), whereas acid precipitation gave the lowest yield (2.8 ± 0.12 g/L) and required longer time (30 days). Finally, the antimicrobial activity of several concentrations of rhamnolipid was tested using modified microdilution method and highest antibacterial activity (in the form of percent reduction in growth) of 95.05% and 91.89% was recorded for Escherichia coli ATCC 10536 and Staphylococcus aureus ATCC 11632, respectively, at 100 µg/mL concentration of rhamnolipid biosurfactant. Conclusion, significance and impact of study: The ability of P. aeruginosa UKMP14T in producing rhamnolipid biosurfactant was confirmed. Despite the higher yield obtained by organic solvent extraction method, the recovery technique (involving the separation of solvent system) caused some loss in product. In addition, the transfer and storage of rhamnolipid was challenging using solvent extraction in comparison to acid precipitation and zinc sulphate precipitation. On the other hand, recovery using acid precipitation suffered from lowest yield of rhamnolipid. Therefore, zinc sulphate precipitation is prioritised over the other two methods. Furthermore, the antimicrobial potential of rhamnolipid biosurfactant was tested successfully for as low as 10 µg/mL concentration against E. coli ATCC 10536 and S. aureus ATCC 11632. Therefore, the recovery cost of a high...

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SPECTROSCOPIC ANALYSIS OF RHAMNOLIPID PRODUCED BY PRODUCED BY Pseudomonas aeruginosa UKMP14T

Cited by 21 publications

References 25 publications

Rhamnolipids Nano-Micelles as a Potential Hand Sanitizer

Rhamnolipids Nano-Micelles as a Potential Hand Sanitizer

Novel Bioformulations Developed from Pseudomonas putida BSP9 and Its Biosurfactant for Growth Promotion of Brassica juncea (L.)

Extraction and antimicrobial activity of rhamnolipid biosurfactant produced by Pseudomonas aeruginosa UKMP14T

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