Spectroscopic and Electrochemical Studies of DNA Breakage Induced by Dopamine and Copper Ion

Li, Jun; Li, Qingwen; Yu, Yadong; Fang, Xiang

doi:10.2116/analsci.19.1099

Cited by 15 publications

(10 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast, Ar + is more likely to cause heavy damage via single-and double-strand breaks in DNA. The in vitro and in vivo effects of �arious ions on DNA breakage and mutation ha�e been pre�iousl� confirmed (Nordhoff et al, 1993;Sandstrom et al, 1994;Kiefer et al, 2002;Liu et al, 2003), but our results suggest that N + is the optimal ion for inducing mutations in microorganisms. A highly active, thermostable α�am�lase �ould be beneficial to the fermentation industry.…”

Section: Discussionsupporting

confidence: 50%

Improved thermostable α-amylase activity of Bacillus amyloliquefaciens by low-energy ion implantation

Zhang²,

Zhu³

2011

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Thermostable α-amylase is of great importance in the starch fermentation industry; it is extensively used in the manufacture of beverages, baby foods, medicines, and pharmaceuticals. Bacillus amyloliquefaciens produces thermostable α�am�lase� ho�e�er� produc� α�am�lase� ho�e�er� produc� -amylase; however, production of thermostable α-amylase is limited. Ion-beam implantation is an effective method for mutation breeding in microbes. We conducted ionbeam implantation experiments using two different ions, Ar + and N + , to determine the survival rate of and dose effect on a high α-amylase activity strain of B. amyloliquefaciens that had been isolated from soil samples. N + implantation resulted in a higher survival rate than Ar + implantation. The optimum implantation dose was 2.08 × 10 15 ions/ cm 2 . Under this implantation condition, we obtained a thermally and genetically stable mutant α-amylase strain (RL-1) with high enzyme activity for degrading α-amylase. Compared to the parental strain (RL), the RL-1 strain had a 57.1% increase in α-amylase activity. We conclude that ion implantation in B. amyloliquefaciens can produce strains with increased production of thermostable α-amylase.

show abstract

Section: Discussionsupporting

confidence: 50%

Improved thermostable α-amylase activity of Bacillus amyloliquefaciens by low-energy ion implantation

Zhang²,

Zhu³

2011

Genet. Mol. Res.

View full text Add to dashboard Cite

show abstract

“…The CD study has been performed to further evaluate the structural modification of DNA caused by an interaction with complex 3. The CT-DNA exhibited two consecutive bands, a positive band at 275 nm due to stacking and a negative band at 245 nm due to helicity in the UV region [50]. Changes in the CD spectrum of CT-DNA in the presence of complex 3 are depicted in Fig.…”

mentioning

confidence: 93%

Chiral and Achiral Macrocyclic Copper(II) Complexes: Synthesis, Characterization, and Comparative Binding Studies with Calf-Thymus DNA

Chauhan

Arjmand

2006

C&B

View full text Add to dashboard Cite

The new chiral macrocyclic complexes [1,2-bis(1H-benzimidazol-2-yl)-1- (1,8-dihydro-1,3,5,8,10,12-hexaazacyclotetradecane)-2-hydroxyethanolate] copper(II) and -nickel(II) perchlorate, 3 and 4, respectively, were synthesized by the reaction of 1,2-bis(1H-benzimidazol-2-yl)ethane-1,2-diol (L) and (1,8-dihydro-1,3,5,8,10,12-hexaazacyclotetradecane)copper(II) and -nickel(II) diperchlorate complexes, 1 and 2, respectively. All complexes were characterized by various spectroscopic techniques. Molarconductance measurements showed that all of the complexes are ionic in nature. In complexes 3 and 4, the metal center is encapsulated by the ligand L in a pentacoordinated environment. The optical-rotation values ([a] D ) of 3 and 4 at 258 indicate that the complexes are chiral. Absorption-and fluorescencespectral studies, cyclic voltammetry, and viscosity measurements have been carried out to assess the comparative binding of complexes 1 and 3 with calf thymus (CT)-DNA. Analysis of the results suggests that the new chiral complex 3 binds to CT-DNA through a partial intercalation mode that is different from the binding mode of parent achiral complex 1. The complexes 1 and 3 bind to CT-DNA with binding constants K b of 2.7 Â 10 4 and 6.6 Â 10 4 m À 1 , respectively. Circular-dichroism (CD) studies have been further employed to ascertain the binding mode of complex 3, which is consistent with the other spectral studies.

show abstract

“…9) Cleavage of the DNA strand by oxidative damage has also been suggested to cause this conformational change. 10) However, our data strongly suggest that the conformational change is independent of oxidative damage, because catalase inhibited 8-oxodG formation without any effect on the CD spectrum, and actually H 2 O 2 was shown to be dispensable for the CD change.…”

Section: Discussionmentioning

confidence: 64%

Involvement of DNA Conformational Change Induced by Rearrangement of Copper-coordination Geometry in Oxidative DNA Damages Caused by Copper and Dopamine

Ando

Ueda

Makino

et al. 2009

JOURNAL OF HEALTH SCIENCE

View full text Add to dashboard Cite

Oxidative DNA damages involve reductionoxidation reactions between certain metals and compounds, and result in genetic abnormalities leading to diseases including cancer and neurodegenerative disorders. Here, we report that DNA damages mediated by combination of a neurotransmitter dopamine and copper accompany a unique DNA conformational change. Copper(II) [Cu(II)] was shown as bound to consecutive guanines (G-G) of DNA, by demonstrating the G-G selective inhibition of restriction enzymes. When dopamine was added to calf thymus DNA in the presence of Cu(II), DNA guanines were oxidized. UV and 1 H NMR analyses suggested that dopamine was oxidized to the quinone form in the presence of Cu(II). The circular dichroism experiments showed DNA spectral changes under the conditions of oxidative DNA damage, indicating a conformational change of the DNA strand by dopamine and copper. The DNA conformational change was suppressed by adding a Cu(I)-specific chelator bathocuproine. Therefore, the conformational change can be explained by a rearrangement of the copper-coordination geometry in DNA through Cu(II) reduction to Cu(I). Thus, changes in DNA conformation could be involved in metal-mediated genotoxicity as well as oxidative damage but via different mechanism.

show abstract

Spectroscopic and Electrochemical Studies of DNA Breakage Induced by Dopamine and Copper Ion

Cited by 15 publications

References 15 publications

Improved thermostable α-amylase activity of Bacillus amyloliquefaciens by low-energy ion implantation

Improved thermostable α-amylase activity of Bacillus amyloliquefaciens by low-energy ion implantation

Chiral and Achiral Macrocyclic Copper(II) Complexes: Synthesis, Characterization, and Comparative Binding Studies with Calf-Thymus DNA

Involvement of DNA Conformational Change Induced by Rearrangement of Copper-coordination Geometry in Oxidative DNA Damages Caused by Copper and Dopamine

Contact Info

Product

Resources

About