Spectroscopic and microscopic characterization of biosensor surfaces with protein/amino-organosilane/silicon structure

Awsiuk, Kamil; Bernasik, Andrzej; Κιτσαρά, Μαρία; Budkowski, Andrzej; Petrou, Panagiota; Kakabakos, Sotirios; Prauzner-Bechcicki, Szymon; Rysz, Jakub; Raptis, Ioannis

doi:10.1016/j.colsurfb.2011.10.017

Cited by 41 publications

(17 citation statements)

References 34 publications

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“…Silicon surfaces are activated with silane selfassembled monolayers prior to their biofunctionalization [97]. Therefore, surface analysis methods including TOF-SIMS have been applied to examine the antibodies, antigens, and blocking proteins adsorbed and covalently attached to silicon surfaces functionalized with organo-silanes for silicon-based biosensors [34,74,[98][99][100]. In the following section, we discuss the TOF-SIMS examination of the factors affecting antibodies orientation, with focus on the surfaces modified with self-assembled monolayers, in particular the organo-silanes on silicon surfaces.…”

Section: Tof-sims Examination Of Protein Conformation Changesmentioning

confidence: 99%

Controlling orientation, conformation, and biorecognition of proteins on silane monolayers, conjugate polymers, and thermo-responsive polymer brushes: investigations using TOF-SIMS and principal component analysis

2020

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Control over orientation and conformation of surface-immobilized proteins, determining their biological activity, plays a critical role in biointerface engineering. Specific protein state can be achieved with adjusted surface preparation and immobilization conditions through different types of protein-surface and protein-protein interactions, as outlined in this work. Time-of-flight secondary ion mass spectroscopy, combining surface sensitivity with excellent chemical specificity enhanced by multivariate data analysis, is the most suited surface analysis method to provide information about protein state. This work highlights recent applications of the multivariate principal component analysis of TOF-SIMS spectra to trace orientation and conformation changes of various proteins (antibody, bovine serum albumin, and streptavidin) immobilized by adsorption, specific binding, and covalent attachment on different surfaces, including self-assembled monolayers on silicon, solution-deposited polythiophenes, and thermo-responsive polymer brushes. Multivariate TOF-SIMS results correlate well with AFM data and binding assays for antibody-antigen and streptavidin-biotin recognition. Additionally, several novel extensions of the multivariate TOF-SIMS method are discussed.

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Section: Tof-sims Examination Of Protein Conformation Changesmentioning

confidence: 99%

Controlling orientation, conformation, and biorecognition of proteins on silane monolayers, conjugate polymers, and thermo-responsive polymer brushes: investigations using TOF-SIMS and principal component analysis

2020

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“…As mentioned in the introduction, surface modification was one of the first key components that were identified from the very beginning as essential in mimicking successfully the micro/nanoenvironment of an organ, and in our case the myocardium, or any other part of the heart [2,3]. Apart from the control of wettability [19], reflectivity [79], plasma surface modification has been used for the immobilisation of proteins on surfaces [17,80] or for the control of attachment, shape and behaviour of various cells [8,9].…”

Section: Plasma Surface Modificationmentioning

confidence: 99%

Heart on a chip: Micro-nanofabrication and microfluidics steering the future of cardiac tissue engineering

Κιτσαρά

Kontziampasis

Agbulut

et al. 2019

Microelectronic Engineering

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“…However, an alternative, although less effective, coupling mechanism was also identified with protonated amino groups pointing toward (charged) silicon. The interaction between amino and surface hydroxyl groups makes it difficult to form ordered layers as NH 2 groups act like build‐in catalyst for the reaction of alkoxy and chlorosilane . When the functionalities to be introduced conflict with the silanization process, an alternative approach is to modify a less problematic silane monolayer using chemical surface reactions.…”

Section: Surface Functionalization Of Silicon Slidesmentioning

confidence: 99%

Interferometric silicon biochips for label and label‐free DNA and protein microarrays

et al. 2012

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Protein and DNA microarrays hold the promise to revolutionize the field of molecular diagnostics. Traditional microarray applications employ labeled detection strategies based on the use of fluorescent and chemiluminescent secondary antibodies. However, the development of high throughput, sensitive, label-free detection techniques is attracting attention as they do not require labeled reactants and provide quantitative information on binding kinetics. In this article, we will provide an overview of the recent author's work in label and label-free sensing platforms employing silicon/silicon oxide (Si/SiO(2)) substrates for interferometric and/or fluorescence detection of microarrays. The review will focus on applications of Si/SiO(2) with controlled oxide layers to (i) enhance the fluorescence intensity by optical interferences, (ii) quantify with sub-nanometer accuracy the axial locations of fluorophore-labeled probes tethered to the surface, and (iii) detect protein-protein interactions label free. Different methods of biofunctionalization of the sensing surface will be discussed. In particular, organosilanization reactions for monodimensional coatings and polymeric coatings will be extensively reviewed. Finally, the importance of calibration of protein microarrays through the dual use of labeled and label-free detection schemes on the same chip will be illustrated.

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Spectroscopic and microscopic characterization of biosensor surfaces with protein/amino-organosilane/silicon structure

Cited by 41 publications

References 34 publications

Controlling orientation, conformation, and biorecognition of proteins on silane monolayers, conjugate polymers, and thermo-responsive polymer brushes: investigations using TOF-SIMS and principal component analysis

Controlling orientation, conformation, and biorecognition of proteins on silane monolayers, conjugate polymers, and thermo-responsive polymer brushes: investigations using TOF-SIMS and principal component analysis

Heart on a chip: Micro-nanofabrication and microfluidics steering the future of cardiac tissue engineering

Interferometric silicon biochips for label and label‐free DNA and protein microarrays

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