Spectroscopic and molecular modeling studies of the interaction between cytidine and human serum albumin and its analytical application

Abstract

In this study, the interaction between cytidine and human serum albumin (HSA) was investigated for the first time by fluorescence spectroscopy in combination with UV absorption spectrum and molecular modeling under simulative physiological conditions. Experimental results indicated that cytidine had a strong ability to quench the intrinsic fluorescence of human serum albumin. The binding constants (K) at different temperatures, thermodynamic parameter enthalpy changes (DeltaH) and entropy changes (DeltaS) of HSA-cytidine had been calculated according to the relevant fluorescence data, which indicated that the hydrophobic and electrostatic interactions played a major role, which was in agreement with the results of molecular modeling study. In addition, the effects of other ions on the binding constants were also studied. Furthermore, synchronous fluorescence technology was successfully applied to the determination of human serum albumin added into the cytidine solution.

Neuroprotective Properties of Citicoline: Facts, Doubts and Unresolved Issues

“…In this context, one should consider the possibility of binding of citicoline to plasma proteins such as albumin. Albumin binding has been reported for cytidine [74] and also for sphingosylphosphorylcholine [75], a compound remotely similar to citicoline. If intact citicoline binds to albumin, its hydrolysis could be retarded and its action as a signalling molecule could be prolonged.…”

Direct In Situ Observation and Numerical Simulations of Non-Shrinking-Core Behavior in an MCMB Graphite Composite Electrode

“…Indeed, experiment has shown that at least with certain highly anisotropic materials, such as LiFePO4, the shrinking core model is not valid. [4][5][6] On the other hand, the model is widely assumed to be correct for larger spherically-shaped agglomerated secondary particles, 5 that is, agglomerated particles can be assumed to be approximately isotropic and homogeneous on the size scale of the particle. Recently, however, some data have been presented that contradicts this approximation.…”