Spectroscopic, cytotoxicity and molecular docking studies on the interaction between 2,4-dinitrophenylhydrazine derived Schiff bases with bovine serum albumin

Behera, Susanta Kumar; Behura, R.; Mohanty, Monalisa; Dinda, Rupam; Mohanty, Patitapaban; Verma, Anil Kumar; Sahoo, Suban K.; Jali, Bigyan R.

doi:10.1016/j.sintl.2020.100048

Cited by 24 publications

(12 citation statements)

References 52 publications

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“…The BSA protein consists of 583 amino acid residues, which can be categorized into three homologous major domains (I, II and III). 37 The complexes were docked within the hydrophobic cavity created by the domains IIA and IIIA. The free energy of binding of the complexes with BSA was found between −5.05 to −6.54 kcal mol −1 , where the IrL1 complex showed the higher affinity with BSA and the docked complex was stabilized by multiple non-covalent interactions (Fig.…”

Section: Resultsmentioning

confidence: 99%

Iridium(iii)–Cp*-(imidazo[4,5-f][1,10]phenanthrolin-2-yl)phenol analogues as hypoxia active, GSH-resistant cancer cytoselective and mitochondria-targeting cancer stem cell therapeutic agents

et al. 2022

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Section: Resultsmentioning

confidence: 99%

Iridium(iii)–Cp*-(imidazo[4,5-f][1,10]phenanthrolin-2-yl)phenol analogues as hypoxia active, GSH-resistant cancer cytoselective and mitochondria-targeting cancer stem cell therapeutic agents

et al. 2022

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“…Fluorescence spectroscopy can also be used to analyze the binding mechanism of chromophores with other compounds. [ 50 ] The ability of a molecule to quench BSA fluorescence intensity depends on the interaction between the tyrosine (Tyr) and tryptophan (Trp) residues in proteins. The interaction is either enhanced or stabilized by the surrounding amino acid residues.…”

Section: Resultsmentioning

confidence: 99%

Silver(I) pyridinyl complexes with benzothiazole, thiophene, and furan moieties: DNA/protein‐binding, antibacterial, antioxidant, and anticancer studies

Adeleke

Islam

Omondi

2022

Archiv der Pharmazie

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We have synthesized and characterized nine Ag(I) complexes of Schiff bases containing thiophene, furan, and pyridine moieties for in vitro antibacterial, antioxidant, anticancer activities, and DNA/bovine serum albumin (BSA) binding studies. Based on the analytical and spectral analyses, a linear geometry was proposed for all the Ag(I) complexes, except for one (with the furan moiety), which formed a distorted T‐shaped geometry. UV‐vis absorption studies on the interactions of calf thymus‐DNA (CT‐DNA) with the nine Ag(I) complexes pointed to an intercalative binding mode. With a binding constant Kb of 3.75 × 105 M−1, the complex bearing a benzothiazole moiety (1) interacted stronger with CT‐DNA than the rest of the complexes. Fluorescence spectroscopic data revealed that the complexes had a modest binding affinity for BSA through static quenching. The complexes displayed good antioxidant properties, especially those with a benzothiazole moiety. Notable antibacterial activities against methicillin‐resistant Staphylococcus aureus, Staphylococcus aureus, Salmonella typhimurium, Pseudomonas aeruginosa, Escherichia coli, and Klebsiella pneumoniae were observed for complexes with the furan and thiophene moieties. The in vitro anticancer studies of selected complexes against three cancer cell lines showed that the complexes were more effective against the inhibition of the growth of cervical cancer cells relative to cisplatin.

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“…The emission wavelength was maintained at 533 nm and the excitation wavelength at 350 nm; the experiment was allowed for the completion of fluorescence polarization anisotropy. Eq was used to determine the steady-state anisotropy ( r ) r = ( I V V − G I V H ) / ( I V V + 2 G I V H ) where I VV and I VH stand for the intensities obtained with the excitation polarizer in the vertical position and the emission polarizer in both the horizontal and vertical positions, respectively. The instrumental grating factor ( G ) is defined as G = I normalH normalV / I normalH normalH where I is designated as the same specifications that were previously given for the excitation polarizer in its horizontal position.…”

Section: Computational Detailsmentioning

confidence: 99%

Anticancer Behavior of Pyrrolidine-Based Palladium(II) Complexes and Biophysical Approach on Their DNA, BSA Binding Activity, Molecular Docking, and DFT Study

et al. 2023

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A series of pyrrolidine-based Pd(II) complexes, [Pd(AEP)Cl 2 ] (C-1), [Pd(AEP)(OH 2 ) 2 ] 2+ (C-2), [Pd(AEP)(Lcys)] + (C-3), [Pd(AEP)(N-ac-L-cys)] (C-4), [Pd(AEP)(GSH)] (C-5), and [Pd(AEP)(DL-meth)] 2+ (C-6) (where, AEP = 1-(2aminoethyl)pyrrolidine, L-cys = L-cysteine, N-ac-L-cys = N-acetyl-L-cysteine, GSH = glutathione, and DL-meth = DL-methionine), as anticancer drug candidates have been synthesized and characterized. The DNA binding property of the complexes was executed by gel electrophoresis and spectrophotometric and viscometric methods, and their interaction with BSA was also investigated by various spectroscopic methodologies. The binding activity of the Pd(II) complexes with DNA and BSA were assessed to evaluate their binding mode and binding constants. Molecular docking was performed to correlate with the experimental results on the interaction of the complexes with DNA and BSA. The changes in the microenvironmental and structural properties of BSA are monitored by a synchronous and 3D fluorescence study. The structural properties were evaluated by DFT and TD-DFT studies. The anticarcinogenic activity of the Pd(II) complexes was assessed by PASS prediction software to corroborate with the experimental results of the anticancer activity of the complexes. The ROS generation in cancer cell lines has been investigated, and the cell death mechanism through apoptosis was confirmed by measuring the protein expression. All these complexes have excellent anticancer activity compared to ancillary ligands. The cancer cell line (HCT116) shows almost similar or better cell inhibition activity when treated with the Pd(II) complexes compared to cisplatin, whereas the adverse effect is minimum on a normal cell (NKE). Both the Pd(II) and Pt(II) complexes carrying the same ligands reveal almost similar antiproliferative activity.

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Spectroscopic, cytotoxicity and molecular docking studies on the interaction between 2,4-dinitrophenylhydrazine derived Schiff bases with bovine serum albumin

Cited by 24 publications

References 52 publications

Iridium(iii)–Cp*-(imidazo[4,5-f][1,10]phenanthrolin-2-yl)phenol analogues as hypoxia active, GSH-resistant cancer cytoselective and mitochondria-targeting cancer stem cell therapeutic agents

Iridium(iii)–Cp*-(imidazo[4,5-f][1,10]phenanthrolin-2-yl)phenol analogues as hypoxia active, GSH-resistant cancer cytoselective and mitochondria-targeting cancer stem cell therapeutic agents

Silver(I) pyridinyl complexes with benzothiazole, thiophene, and furan moieties: DNA/protein‐binding, antibacterial, antioxidant, and anticancer studies

Anticancer Behavior of Pyrrolidine-Based Palladium(II) Complexes and Biophysical Approach on Their DNA, BSA Binding Activity, Molecular Docking, and DFT Study

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