Spectroscopic Detection of Pathogens

Alam, Mary Kathleen; Timlin, Jerilyn A.; Martin, Laura E.; Hjelle, Drian; Lyons, R W; Garrison, Kristin

doi:10.2172/771503

Cited by 2 publications

(2 citation statements)

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“…Chemical changes in erythrocytes due to oxidative stress in athletes have also been recently studied by infrared spectroscopy [24][25][26]. The histocompatibility testing of bone marrow transplants [19] and the monitoring of macromolecular changes associated with the onset of immune cell activation is also an interesting field of study [18,20].…”

Section: Blood Cell Characterisationmentioning

confidence: 99%

Transmission infrared spectroscopy of whole blood – complications for quantitative analysis from leucocyte adhesion during continuous monitoring

Vahlsing

Damm

Kondepati

et al. 2010

Journal of Biophotonics

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Infrared spectroscopy has been applied to analyse glucose and cellular components in whole blood with the aim of developing an online clinical diagnostic and monitoring modality. Leucocyte adsorption onto the CaF(2) windows was observed over a period of several hours under continuous blood flow using a transmission cell of 30 mum path length. This build-up of cellular material on the windows is responsible for diminishing the sample path length under the flow conditions chosen. The adsorption dynamics have been characterised and their impact on glucose monitoring is reported. For short-term monitoring (<2 hours) a standard error of prediction of 11 mg/dL with human citrated blood samples from three different subjects was achieved. Furthermore, the leucocyte build-up was also reported for porcine EDTA blood monitoring. Consequences and testing opportunities with regard to the first stages in the immune cell reaction to the exposure of body-foreign materials to anticoagulated whole blood are discussed.

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Section: Blood Cell Characterisationmentioning

confidence: 99%

Transmission infrared spectroscopy of whole blood – complications for quantitative analysis from leucocyte adhesion during continuous monitoring

Vahlsing

Damm

Kondepati

et al. 2010

Journal of Biophotonics

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show abstract

“…Infrared spectroscopy has emerged as a powerful characterization tool in biomedical diagnostics providing sensitivity to the variation of the molecular sample composition and has been applied for rapid classification of cell and tissue samples. [6][7][8][9][10][11][12][13][14][15][16][17][18] For example, higher concentrations of lipids in atherosclerotic plaque in contrast to normal artery tissue 19 have been confirmed by different IR techniques, including conventional transmission Fourier transform infrared (FT-IR) spectroscopy, 1 FT-IRattenuated total reflectance (ATR) spectroscopy, 2,3 and FT-IR microspectroscopy. 4 Among these techniques, ATR methods are of particular interest due to their lack of dependence on sample thickness, which renders them ideal for thick and strongly absorbing materials such as tissue.…”

Section: Introductionmentioning

confidence: 99%

Classification of Atherosclerotic Rabbit Aorta Samples with an Infrared Attenuated Total Reflection Catheter and Multivariate Data Analysis

Wang

Chapman²,

Palmer³

et al. 2006

Appl Spectrosc

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The strongly overlapping infrared absorption features of atherosclerotic and normal rabbit aorta samples as governed by their water, lipid, and protein content render the direct evaluation of molecular characteristics obtained from infrared (IR) spectroscopic measurements challenging for classification. We have successfully applied multivariate data analysis and classification techniques based on partial least squares regression (PLS), linear discriminant analysis (LDA), and principal component regression (PCR) to IR spectroscopic data obtained by using a recently developed infrared attenuated total reflectance (IR-ATR) catheter prototype for future in vivo diagnostic applications. Training data were collected ex vivo from atherosclerotic and normal rabbit aorta samples. The successful classification results on atherosclerotic and normal aorta samples utilizing the developed data evaluation routines reveals the potential of spectroscopy combined with multivariate classification strategies for the identification of normal and atherosclerotic aorta tissue for in vitro and, in the future, in vivo applications.

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Spectroscopic Detection of Pathogens

Cited by 2 publications

References 0 publications

Transmission infrared spectroscopy of whole blood – complications for quantitative analysis from leucocyte adhesion during continuous monitoring

Transmission infrared spectroscopy of whole blood – complications for quantitative analysis from leucocyte adhesion during continuous monitoring

Classification of Atherosclerotic Rabbit Aorta Samples with an Infrared Attenuated Total Reflection Catheter and Multivariate Data Analysis

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