Spectroscopic evidence of the effects induced by non-ionizing radiation on tissue samples

Crupi, V.; Interdonato, S.; Majolino, D.; Mondello, Maria Rita; Venuti, Valentina

doi:10.1016/j.vibspec.2006.05.019

Cited by 2 publications

(1 citation statement)

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“…This technique has gained considerable importance in recent years since it gives the opportunity to fully characterize the structures of proteins, lipids, carbohydrates, polysaccharides, nucleic acids, etc. The characteristic frequencies, intensities, band areas, and band widths in an IR spectrum allow the identification of the functional groups of molecules, providing the conformational distinction of the structures in biological molecules (Crupi et al, 2006). The recent breakthroughs both in the instrumentation and in the data processing have made the FT‐IR spectroscopy as a useful and powerful tool to study the biological molecules.…”

Section: Introductionmentioning

confidence: 99%

A study of the subchronic effects of arsenic exposure on the liver tissues of Labeo rohita using Fourier transform infrared technique

Palaniappan

Vijayasundaram

Prabu

2011

Environmental Toxicology

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In this work, an attempt has been made to study the subchronic effects of arsenic exposure on the biochemical composition; mainly proteins of the liver tissues of Labeo rohita fingerlings by using Fourier transform infrared (FT-IR) spectroscopic technique. The study was carried out using a Perkin Elmer-Spectrum Rx1 spectrometer. Because of arsenic exposure, significant reductions in the intensity as well as area of amide bands have been observed in the liver tissues. The decreased intensity of the amide bands could be interpreted as the result of alteration of the protein synthesis due to the high affinity of metal compounds towards different amino acid residues of proteins. Further, meso-2, 3-dimercaptosuccinic acid (DMSA) treatment shows the recovery of the protein content in the liver tissues. To confirm that the changes observed are only due to the bio-accumulation of arsenic, the concentration of arsenic in the liver tissues of Labeo rohita was determined by using Inductively Coupled Plasma-Optical Emission Spectrometry (ICP-OES). It is observed that the arsenic level in the control tissues is found to be below detectable limit, whereas the arsenic exposed liver shows an accumulation of 66.68 ± 0.43 μg/g and DMSA treatment reduces the arsenic content to 17.96 ± 0.19 μg/g. In conclusion, this study gives clear evidence that the use of FT-IR spectroscopy is a powerful approach to achieve more insight into the protein alterations caused by arsenic.

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Section: Introductionmentioning

confidence: 99%