Spectroscopic examination of enamel staining by coffee indicates dentin erosion by sequestration of elements

Manno, Sinai H. C.; Manno, Francis A. M.; Ahmed, Irfan; Ahmed, Rafay; Shu, Lei; Li, Li; Xu, Shisan; Xie, Fangjing; Li, Vincent Wai; Ho, Johnny C.; Cheng, Shuk Han; Lau, Condon

doi:10.1016/j.talanta.2018.07.032

Cited by 25 publications

(27 citation statements)

References 58 publications

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“…Recently, we demonstrated that spectroscopic techniques have the potential to discriminate elemental changes in teeth treated with coffee [26]. In the present report, we identified the subtle effect that temperature has on teeth treated with tea.…”

Section: Introductionmentioning

confidence: 54%

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Spectroscopic and microscopic examination of teeth exposed to green tea at different temperatures

Manno

Tian

et al. 2020

PLoS ONE

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Tea is a popular beverage consumed at different temperatures. The effect of tea on teeth at different temperatures has not been studied previously. The present study used an in vitro green tea immersed tooth model at different tea temperatures (hot and cold) compared to an in vivo tea administration model allowing rats to drink tea over the course of a week. The elements present in tea leaves were identified by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) and compared to the elements in teeth (enamel surface) using Laser-Induced Breakdown Spectroscopy (LIBS). Here, LIBS demonstrated in vivo and in vitro green tea treatments resulted in a significant increase in the mineral elements found in enamel. For the in vitro assessment, elements in enamel varied based on cold-tea and hot-tea treatment; however, hot water reduced the elements in enamel. Atomic force microscopy found the in vivo tea group had a higher roughness average (RA) compared with the in vivo water group. Cold tea and hot tea in vitro groups demonstrated lower RA than in vitro water controls. Scanning electron microscopy found hot water induced cracks more than 1.3μm in enamel while cold tea and hot tea promoted the adhering of extrinsic matter to teeth. Overall, teeth treated to high temperature lost the mineral phase leading to demineralization. Our results indicate that green tea protects enamel, but its protective action in dental structures is enhanced at cold temperature.

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Section: Introductionmentioning

confidence: 54%

“…We simulated drinking tea for a 5-year period daily for five minutes using a week immersion paradigm based on a back-of-the-envelope estimation inspired by E. Fermi [26]. For in vitro experiments, teeth were immersed for one week in a hot or room temperature (cold) solution of green tea or water.…”

Section: Back-of-the-envelope Calculationsmentioning

confidence: 99%

Spectroscopic and microscopic examination of teeth exposed to green tea at different temperatures

Manno

Tian

et al. 2020

PLoS ONE

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“…Titanium (Ti) and iron (Fe) contents were measured from whole-blood samples based on Association of Analytical Communities 999.10 and determination by ICP-MS. [26][27][28][29] Blood, nitric acid (HNO 3 ), and hydrogen peroxide (H 2 O 2 ) were added to the digestion vessel and digested using a microwave digester at four powers 250, 630, 500, and 0 W for 3, 5, 22, and 15 min, respectively. Then the samples were transported to and analyzed using a PerkinElmer ELAN DRC II ICP-MS.…”

Section: Trace Metal Quantificationmentioning

confidence: 99%

Single red blood cell analysis reveals elevated hemoglobin in poikilocytes

et al. 2020

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Abnormally shaped red blood cells (RBCs), called poikilocytes, can cause anemia. At present, the biochemical abnormalities in poikilocytes are not well understood. Normal RBCs and poikilocytes were analyzed using whole-blood and single-cell methods. Poikilocytes were induced in rat blood by intragastrically administering titanium dioxide (TiO 2) nanoparticles. Complete blood count and inductively coupled plasma mass spectrometry analyses were performed on whole-blood to measure average RBC morphology, blood hemoglobin (HGB), iron content, and other blood parameters. Follow-up confocal Raman spectroscopy was performed on single RBCs to analyze cell-type-specific HGB content. Two types of poikilocytes, acanthocytes and echinocytes, were observed in TiO 2 blood samples, along with normal RBCs. Acanthocytes (diameter 7.7 AE 0.5 μm) and echinocytes (7.6 AE 0.6 μm) were microscopically larger (p < 0.05) than normal RBCs (6.6 AE 0.4 μm) found in control blood samples (no TiO 2 administration). Similarly, mean corpuscular volume was higher (p < 0.05) in TiO 2 whole-blood (70.70 AE 1.97 fl) than in control whole-blood (67.42 AE 2.03 fl). Poikilocytes also had higher HGB content. Mean corpuscular hemoglobin was higher (p < 0.05) in TiO 2 whole-blood (21.84 AE 0.75 pg) than in control whole-blood (20.8 AE 0.32 pg). Iron content was higher (p < 0.001) in TiO 2 whole-blood (697.0 AE 24.5 mg∕l) than in control whole-blood (503.4 AE 38.5 mg∕l), which supports elevated HGB as iron is found in HGB. HGB-associated Raman bands at 1637, 1585, and 1372 cm −1 had higher (p < 0.001) amplitudes in acanthocytes and echinocytes than in RBCs from control blood and normal RBCs from TiO 2 blood. Further, the 1585-cm −1 band had a lower (p < 0.05) amplitude in normal RBCs from TiO 2 versus control RBCs. This represents biochemical abnormalities in normal appearing RBCs. Overall, poikilocytes, especially acanthocytes, have elevated HGB.

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“…LIBS and XRF are complementary methods as together, they are sensitive to elements across much of the periodic Table [ 35 ]. This combined approach has provided fast and multi-element analysis of plant nutrition [ 35 ] and our group has recently explored dental applications [ 36 ]. Also, LIBS can analyze solid, liquid, and gaseous samples while XRF is primarily for solid samples.…”

Section: Introductionmentioning

confidence: 99%

Detection of lithium in breast milk and in situ elemental analysis of the mammary gland

Ahmed¹,

Manno²,

Manno³

et al. 2018

Biomed. Opt. Express

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Breast feeding provides considerable benefits to the infant and mother. However, a lithium-based psychiatric medication may cause side effects in the child. Using laser induced breakdown spectroscopy (LIBS), trace lithium levels were observed in the breast milk of lactating rats administered with lithium treatment postpartum. Subsequently, the mammary glands of female rats were analyzed using LIBS, energy dispersive X-ray fluorescence spectroscopy, and inductively coupled plasma mass spectrometry. Key biological elements iron, magnesium, cobalt, calcium, phosphorus, sodium, iodine, potassium, sulfur, chlorine and zinc were observed. Lithium at 1.06 µg/g was measured in the mammary glands of treated subjects, but was below the limit of detection in controls. Lithium also increased iodine content in the glands. Lithium is present in the breast milk and mammary glands of lithium treated female subjects and this is the likely route of entry to breast-fed infants.

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Spectroscopic examination of enamel staining by coffee indicates dentin erosion by sequestration of elements

Cited by 25 publications

References 58 publications

Spectroscopic and microscopic examination of teeth exposed to green tea at different temperatures

Spectroscopic and microscopic examination of teeth exposed to green tea at different temperatures

Single red blood cell analysis reveals elevated hemoglobin in poikilocytes

Detection of lithium in breast milk and in situ elemental analysis of the mammary gland

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