Spectroscopic investigations on the effect of N-Acetyl-l-cysteine-Capped CdTe Quantum Dots on catalase

Sun, Haoyu; Yang, Bingjun; Cui, Erqian; Liu, Rutao

doi:10.1016/j.saa.2014.04.157

Cited by 38 publications

(18 citation statements)

References 40 publications

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“…But it can be seen that the inhibition effect of Sudan dyes is not significant. One of the possible reasons may be that the porphyrin rings are wrapped in the deep structure of CAT, the two Sudan dyes are hard to interact with it directly . The results are consistent with the analyses of UV–vis absorption spectra.…”

Section: Resultssupporting

confidence: 87%

“…With the addition of Sudan dyes, there is an increasing tendency of the α‐helix content and an opposite tendency of the β‐sheet, which suggest that the interaction between CAT and the two Sudan dyes leads to a shrinkage of the secondary structure . Sudan dyes can bind to the amino acid residues in the main polypeptide chain, destroying the hydrogen bonds in the protein and affecting the function of CAT . It is concluded from the results that both Sudan dyes alter the conformational structure of CAT in a similar way.…”

Section: Resultsmentioning

confidence: 85%

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Comparison of the toxicity of the dyes Sudan II and Sudan IV to catalase

Hao

Pan

et al. 2017

J Biochem & Molecular Tox

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The mechanisms of the toxicity of Sudan dyes to the key antioxidant enzyme catalase (CAT) were investigated by spectroscopic methods, calorimetry techniques, enzyme activity assay, and molecular docking. Results showed that Sudan dyes bound to CAT through hydrophobic force, which changed the microenvironment of tryptophan and tyrosine residues, leading to a conformational alteration and shrinkage of the protein. Enzyme activity assay and molecular docking revealed that the activity of CAT was slightly inhibited in the presence of Sudan dyes. In comparison, the binding of Sudan II with CAT was slightly stronger than Sudan IV. Also, Sudan II and Sudan IV showed a different impact on the microenvironment of aromatic amino acid residues. But the dyes had very similar effects on conformation and activity of the protein. This work provides an essential reference for the evaluation of Sudan dyes' effects on body's antioxidant defense system and safe use of Sudan dyes.

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Section: Resultssupporting

confidence: 87%

Section: Resultsmentioning

confidence: 85%

Comparison of the toxicity of the dyes Sudan II and Sudan IV to catalase

Hao

Pan

et al. 2017

J Biochem & Molecular Tox

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“…The broad bands around 3300–3600 cm −1 in the spectra may arise from the hydroxyl groups and H 2 O bound on the QDs surface. In the curve of NAC, the characteristic bands at 2547.55 cm −1 are attributed to S-H group 21 . Furthermore, the peaks at 1718.29 cm −1 and 1303.66 cm −1 are corresponding to carboxyl group, and the bands at 1190–1170 cm −1 are ascribed to C-N group, 1535.08 cm −1 is due to N-H stretching vibrations of -NH- 22 .…”

Section: Resultsmentioning

confidence: 99%

Aqueous synthesis of functionalized copper sulfide quantum dots as near-infrared luminescent probes for detection of Hg2+, Ag+ and Au3+

Liao

Yang

et al. 2017

Sci Rep

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Stable water-soluble copper sulfide(Cu2S) quantum dots(QDs) with near-infrared emission were synthesized using N-acetyl-L-cysteine(NAC) as a modifier in aqueous solution and nitrogen atmosphere at room temperature. The product was characterized by TEM, XRD, XPS, FT-IR, FL and UV-VIS spectrometers. Effects of preparation conditions such as pH values, the molar ratio of reactants, temperature, and metal ions on the fluorescence properties of Cu2S QDs were discussed. Under optimal conditions, the prepared Cu2S QDs with average diameter about 2–5 nm show a near-infrared emission at 770 nm with the excitation wavelength of 466 nm, and have a good detection sensitivity for ions of Hg2+, Ag+ and Au3+, based on the characteristic of fluorescence quenching. The fluorescence quenching mechanism was proposed via electron transfer with cation exchange, which based on the theory of Hard-Soft-Acid-Base (HSAB) and Ksp value of metal–sulfide.

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“…However, these techniques often include error-prone polymerase chain reaction (epPCR) and in vitro recombination, which are time-consuming, expensive, and tedious [5]. Furthermore, not all enzymes lend themselves to recombinant improvement due to the need to maintain the inherent structure that is associated with enzyme-substrate binding and catalysis [6,7]. Enzyme immobilization onto macro/micro surfaces is also typically required in order to enable their use in non-native applications such as in biosensors, environmental remediation materials, bioreactors, and other applied biotechnology fields [8,9].…”

Section: Introductionmentioning

confidence: 99%

Increasing the activity of immobilized enzymes with nanoparticle conjugation

Ding¹,

Cargill²,

Medintz³

et al. 2015

Current Opinion in Biotechnology

250

153

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The efficiency and selectivity of enzymatic catalysis is useful to a plethora of industrial and manufacturing processes. Many of these processes require the immobilization of enzymes onto surfaces, which has traditionally reduced enzyme activity. However, recent research has shown that the integration of nanoparticles into enzyme carrier schemes has maintained or even enhanced immobilized enzyme performance. The nanoparticle size and surface chemistry as well as the orientation and density of immobilized enzymes all contribute to the enhanced performance of enzyme-nanoparticle conjugates. These improvements are noted in specific nanoparticles including those comprising carbon (e.g., graphene and carbon nanotubes), metal/metal oxides and polymeric nanomaterials, as well as semiconductor nanocrystals or quantum dots.

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Spectroscopic investigations on the effect of N-Acetyl-l-cysteine-Capped CdTe Quantum Dots on catalase

Cited by 38 publications

References 40 publications

Comparison of the toxicity of the dyes Sudan II and Sudan IV to catalase

Comparison of the toxicity of the dyes Sudan II and Sudan IV to catalase

Aqueous synthesis of functionalized copper sulfide quantum dots as near-infrared luminescent probes for detection of Hg2+, Ag+ and Au3+

Increasing the activity of immobilized enzymes with nanoparticle conjugation

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