Spectroscopic properties and amplified spontaneous emission of fluorescein laser dye in ionic liquids as green media

AL-Aqmar, Dalal M.; Abdelkader, H.I.; Kana, Maram T.H. Abou

doi:10.1016/j.optmat.2015.06.045

Cited by 24 publications

(13 citation statements)

References 43 publications

(40 reference statements)

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“…Since there is a large range of different fluorescein derivates in use [ 41 ], the absorption and emission spectra also vary substantially. Our measurements are, however, in good accordance with recently published results of free acid type fluorescein [ 2 , 15 , 19 ], with two characteristic absorption peaks around 450 and 480 nm, and an emission peak around 520 nm for both ethanol and methanol solutions. The present work is to our knowledge the first published on fluorescein absorption and fluorescence properties in a sulfuric acid solution.…”

Section: Resultssupporting

confidence: 92%

Testing Fluorescence Lifetime Standards using Two-Photon Excitation and Time-Domain Instrumentation: Fluorescein, Quinine Sulfate and Green Fluorescent Protein

et al. 2018

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It is essential for everyone working with experimental science to be certain that their instruments produce reliable results, and for fluorescence lifetime experiments, information about fluorescence lifetime standards is crucial. A large part of the literature on lifetime standards dates back to the 1970s and 1980s, and the use of newer and faster measuring devices may deem these results unreliable. We have tested the three commonly used fluorophores fluorescein, quinine sulfate and green fluorescent protein for their suitability to serve as lifetime standards, especially to be used with two-photon excitation measurements in the time-domain. We measured absorption and emission spectra for the fluorophores to determine optimal wavelengths to use for excitation and detector settings. Fluorescence lifetimes were measured for different concentrations, ranging from 10− 3 − 10− 5 M, as well as for various solvents. Fluorescein was soluble in both ethanol, methanol and sulfuric acid, while quinine sulfate was only soluble in sulfuric acid. Green fluorescent protein was prepared in a commercial Tris-HCl, EDTA solution, and all three fluorophores produced stable lifetime results with low uncertainties. No siginificant variation with concentration was measured for any of the fluorophores, and all showed single-exponential decays. All lifetime measurements were carried out using two-photon excitation and lifetime data was obtained in the time-domain using time-correlated single-photon counting.

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Section: Resultssupporting

confidence: 92%

Testing Fluorescence Lifetime Standards using Two-Photon Excitation and Time-Domain Instrumentation: Fluorescein, Quinine Sulfate and Green Fluorescent Protein

et al. 2018

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“…of the long surviving component begins a gradual decrease in intensity and shifts to red range. This finding conformed to our previous works [22][23][24]. Figure 4a-c displays the absorption spectra of IR-792, with various concentrations.…”

Section: Resultssupporting

confidence: 91%

“…Acknowledgments The authors would like to thank Cairo University for Supporting and funding this work through project no. (16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33).…”

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confidence: 99%

Spectroscopic and Photo-Physical Properties of Near-IR Laser Dye in Novel Benign Green Solvents

et al. 2020

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IR-792 as near IR (NIR) laser dye was dissolved with different concentrations in two types of ionic liquids (ILs) of different anion and cation, 1-Ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl) imide (EMIM TFSI) & 1-Butyl-3-methylimidazolium tetrafluoroborate (BMIM BF 4 ), as the benign green solvent and in methanol (MeOH) as a standard solvent. The behavior of fluorescence of IR-792 dye was studied. The fluorescence of IR-792 dissolved in the ILs was heavily compared to organic solvent. Some photo-physical parameters of IR-792 were calculated. Mainly, IR-792 had a very low quantum yield of fluorescence with high intersystem crossing rate & fluorescence lifetime in picosecond range. Optical absorption and behavior of fluorescence for the rigorously the purified imidazolium ILs in the neat condition and effect of IR-792 on their fluorescence have been examined. The emission behavior of IR-792 in green solvents was independent upon the wavelength of excitation, while the emission behavior of green solvents dependent upon the wavelength of excitation whether in pure state or with NIR laser dye. At most, the intensity of fluorescence of ILs is dependent upon dye concentration.

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“…Fluorescein has found application in the production of advanced molecular probes for biological, toxicological, biomedical, and environmental studies (Burchak et al 2006, Oliveira et al 2018, Xiong et al 2014, Zhwng et al 2013. Fluorescein has been used as a lasing medium (green, 530-560 nm) in laser dyes (Al-Aqmar et al 2015, Govindanunny & Sivaram 1980. It is desirable for a dye laser gain medium to possess an appreciably long fluorescence lifetime, and as such, a thorough knowledge of its photophysical properties is worthwhile.…”

Section: Introductionmentioning

confidence: 99%

Determination of fluorescence lifetimes of fluorescein from fluorescence quenching data

Ogunsipe

Opeolu

2020

IJAC

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The fluorescence quenching of fluorescein by 1,4-benzoquinone (BQ) in five solvents – methanol, ethanol, phosphate buffer saline (PBS, pH 7.4), N,N'-dimethylformamide (DMF) and dimethylsulphoxide (DMSO) is hereby investigated. Fluorescein’s fluorescence was effectively quenched by BQ, and the quenching was dynamic (purely collisional) within the BQ’s concentration range (0.001 to 0.004 M) used in this work. Accordingly, the quenching data were in conformity with the Stern-Volmer’s model. Stern-Volmer’s constant (KSV) values range between 17.4 in DMSO and 43.4 in methanol. KSV values, together with the calculated bimolecular rate constants (kD) in the respective solvents, were used to semi-empirically estimate the fluorescence lifetimes (tF) of fluorescein in the individual solvents. Just as for KSV values, tF values are solvent-viscosity dependent, with the lowest values being obtained in methanol and the highest in DMSO, which are the least and most viscous solvents respectively. tF values obtained in this work are 3.55, 3.71, 3.78, 4.13 and 4.51 ns (in methanol, PBS 7.4, ethanol, DMF and DMSO, respectively).

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Spectroscopic properties and amplified spontaneous emission of fluorescein laser dye in ionic liquids as green media

Cited by 24 publications

References 43 publications

Testing Fluorescence Lifetime Standards using Two-Photon Excitation and Time-Domain Instrumentation: Fluorescein, Quinine Sulfate and Green Fluorescent Protein

Testing Fluorescence Lifetime Standards using Two-Photon Excitation and Time-Domain Instrumentation: Fluorescein, Quinine Sulfate and Green Fluorescent Protein

Spectroscopic and Photo-Physical Properties of Near-IR Laser Dye in Novel Benign Green Solvents

Determination of fluorescence lifetimes of fluorescein from fluorescence quenching data

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