Spectroscopic quantification of soil phosphorus forms by 31p-nmr after nine years of organic or mineral fertilization

Abstract

SUMMARYLong-standing applications of mineral fertilizers or types of organic wastes such as manure can cause phosphorus (P) accumulation and changes in the accumulated P forms in the soil. The objective of this research was to evaluate the forms of P accumulated in soils treated with mineral fertilizer or different types of manure in a long-term experiment. Soil was sampled from the 0-5 cm layer of plots fertilized with five different nutrient sources for nine years: 1) control without fertilizer; 2) mineral fertilizer at recommended rates for local conditions; 3) 5 t ha -1 year -1 of moist poultry litter; 4) 60 m 3 ha -1 year -1 of liquid cattle manure and 5) 40 m 3 ha -1 year -1 of liquid swine manure. The 31 P-NMR spectra of soil extracts detected the following P compounds: orthophosphate, pyrophosphate, inositol phosphate, glycerophosphate, and DNA. The use of organic or mineral fertilizer over nine years did not change the soil P forms but influenced their concentration. Fertilization with mineral or organic fertilizers stimulated P accumulation in inorganic forms. Highest inositol phosphate levels were observed after fertilization with any kind of manure and highest organic P concentration in glycerophosphate form in after mineral or no fertilization.Index terms: glycerophosphate, manure, phytate, organic phosphorus, organic fertilization.(1) Received for publication on October 26, 2011 and approved on January 22, 2013.

A31P-NMR spectroscopic study of phosphorus forms in two phosphorus-fertilized grassland soils in eastern Canada

“…Pyrophosphate and polyphosphate concentrations were not significantly affected by P fertilization (Table 7). Similar results were obtained by Condron et al (1985), Gatiboni et al (2013), and Cade-Menun et al (2017) with long-term fertilized soils. Pyrophosphate is a short-chain polyphosphate (n = 2), and is believed to be a product of biological P cycling in soils (Condron et al 2005).…”

“…Solution 31 P nuclear magnetic resonance spectroscopy ( 31 P-NMR) is an advanced spectroscopic technique widely used to identify P compounds in soil extracts (Cade-Menun and Preston 1996;Cade-Menun 2005; Cade-Menun and Liu 2014). Gatiboni et al (2013) assessed the P compounds identified by 31 P-NMR spectroscopy in a Brazilian soil treated with mineral P fertilizer for 9 yr. They reported an accumulation of P i and inositol phosphate in the top 5 cm soil depth.…”

Engineering l-asparaginase for spontaneous formation of calcium phosphate bioinspired microreactors

“…The high protein loading at the surface reduces the achievable resolution. 55 (this peak cannot be attributed to betaglycerophosphate because it exhibits a higher chemical shift 56 ). Mono-acid phosphates were shown to adopt a large range of chemical shifts, depending on the surroundings ions.…”

Advancements in waste water characterization through NMR spectroscopy: review

“…It was proposed that 31 P NMR is the most reliable method for the detection and quantification of polyphosphate species in environmental samples. In 2013, Gatiboni et al [104] performed quantification using 31 P NMR to study soil phosphorus forms after 9 years of organic, such as manure, or mineral fertilization. They showed that the use of fertilizers over 9 years did not change the soil phosphorus forms but influenced their concentration.…”