Spectroscopic studies on the interaction between riboflavin and albumins

Zhao, Hongwei; Min, Gao; Zhang, Zhaoxia; Wang, Wenfeng; Wu, Guozhong

doi:10.1016/j.saa.2005.12.038

Cited by 127 publications

(55 citation statements)

References 37 publications

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“…Quenching of fluorescence can occur via several processes, including energy transfer, excited-state reactions, collisional quenching and static quenching. 46 Collisional quenching occurs when the fluorophore and quencher interact during the excitation of the fluorophore, whereas static quenching occurs during the formation of a fluorophore-quencher complex in the ground state. 46 The…”

Section: Dalton Transactions Papermentioning

confidence: 99%

Synthesis and analysis of the anticancer activity of platinum(ii) complexes incorporating dipyridoquinoxaline variants

Pages

Wormell

et al. 2014

Dalton Trans.

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Eight platinum(II) complexes with anticancer potential have been synthesised and characterised. These complexes are of the type [, where I L is either dipyrido[3,2-f:2',3'-h]quinoxaline (dpq) or 2,3-dimethyl-dpq (23Me 2 dpq) and A L is one of the R,R or S,S isomers of either 1,2-diaminocyclohexane (SS-dach or RR-dach) or 1,2-diaminocyclopentane (SS-dacp or RR-dacp). The CT-DNA binding of these complexes and a series of other complexes were assessed using fluorescent intercalator displacement assays, resulting in unexpected trends in DNA binding affinity. The cytotoxicity of the eight synthesised compounds was determined in the L1210 cell line; the most cytotoxic of these were [Pt(dpq)(SS-dach)]Cl 2 and [Pt(dpq)(RR-dach)]Cl 2 , with IC 50 values of 0.19 and 0.80 μM, respectively. The X-ray crystal structure of the complex [Pt(dpq)(SS-dach)](ClO 4 ) 2 ·1.75H 2 O is also reported.

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Section: Dalton Transactions Papermentioning

confidence: 99%

Synthesis and analysis of the anticancer activity of platinum(ii) complexes incorporating dipyridoquinoxaline variants

Pages

Wormell

et al. 2014

Dalton Trans.

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“…10) For static quenching, when small molecules bind independently to a set of equivalent sites on a macromolecule, the equilibrium between free and bound molecules is described by the Scatchard equation or Binding equation, Eq. 2 [11][12][13] :…”

Section: Methodsmentioning

confidence: 99%

Interaction Characteristics with Bovine Serum Albumin and Retarded Nitric Oxide Release of ZCVI4-2, a New Nitric Oxide-Releasing Derivative of Oleanolic Acid

Zhang

Gao

et al. 2011

CHEMICAL & PHARMACEUTICAL BULLETIN

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Human hepatocellular carcinoma (HCC) is one of the leading causes for mortality in the world and there is no effective chemotherapy at present. Therefore, development of new therapeutically effective and low toxic reagents, particularly injectable drugs, will be of great significance. ZCVI 4 -2 ( Fig. 1) is a newly developed furoxan-based nitric oxide-releasing derivative of oleanolic acid, and has been successfully synthesized as galactosyl derivative 3 of the compound 1 (furoxan-based nitric oxide-releasing derivative of oleanolic acid) by Huang et al. 1) As reported previously, it exhibited strong cytotoxicity against HCC in vitro and significantly inhibited the growth of HCC tumors in vivo, indicating that ZCVI 4 -2 may be a promising candidate for the treatment of human HCC. 1) However, ZCVI 4 -2 has a very low aqueous solubility (Ͻ10 mg/ml at pH 3.0-9.0). The poor solubility of ZCVI 4 -2 has been a major hindrance in the development of its parenteral dosage forms. In our previous research, the combination of macrogol 15 hydroxystearate (Solutol HS-15), propylene glycol and ethanol was used to improve its solubility successfully to be up to 5 mg/ml. In vivo activity evaluation indicated that ZCVI 4 -2 treatment with 25 mg/kg of ZCVI 4 -2 enhanced the inhibitory effect on the growth of HCC tumors SMMC-7721 transplanted in nude mice ( pϽ0.01 vs. treatment with 12.5 mg/kg), and its inhibitory effect was similar to that of 5-FU treatment. The tumor weights in the mice treated with ZCVI 4 -2 at 12.5 or 25 mg/kg was significantly lower than that from the vehicletreated controls (pϽ0.01). However, ZCVI 4 -2 injection displayed obvious toxicity to mice in toxicological studies with a LD 50 value of 94.1 mg/kg. 1) By comparing the mice survival of ZCVI 4 -2 suspension with ZCVI 4 -2 injection, it was inferred that the toxicity of ZCVI 4 -2 may be attributed firstly to the fast release of nitric oxide (NO) in blood and secondly to the surfactant and organic solvents in the formulation.The serum albumin was used frequently for the preparation of nanoparticles because of its excellent water-solubility and biocompatibility. Human serum albumin (HSA) had been successfully bound with paclitaxel and formulated into Abraxane ® , the paclitaxel protein-bound particles for injectable suspension. The absence of organic solvents and surfactants significantly improved the clinical safety.2-5) The key step to prepare the protein (bovine serum albumin (BSA) or HSA)-bound nanocomplex is to determine the rational condition for the binding between drug and protein through interaction study. To date, however, the interaction characteristics, the binding condition for paclitaxel and HSA, the screening process of formulation and technology of Abraxane ® were not reported. To delay the release of the NO, and to lower the toxicity after injection, the serum albumin (BSA) was utilized for the preparation of the surfactant-free serum albumin nanocomplexes of ZCVI 4 -2 in this study. The main objective of the present study is to study the in...

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“…This protein does not affect other enzymes that do not need it for stabilization. The binding properties of BSA with various drugs have been fully investigated by many researchers [23][24][25]. In this present work, interaction between suspended H 2 Ti 3 O 7 nanotubes (in aqueous solution) and CT-DNA, followed by BSA protein and was examined by UV and fluorescence spectroscopy.…”

Section: Introductionmentioning

confidence: 95%

Study of Photoinduced Interaction between Calf Thymus-DNA and Bovine Serum Albumin Protein with H2Ti3O7 Nanotubes

Chakraborty¹,

Sarkar²,

Chattopadhyay³

2012

JBNB

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Hydrogen titanate nanotubes were synthesized by hydrothermal process using 10 M NaOH and TiO 2 anatase powder. The material synthesized was characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM) to ensure the structural and morphological characteristics. The interaction of calf thymus DNA (CT-DNA) and bovine serum albumin protein with suspended aqueous solution of titanate nanotubes was investigated using UV absorption spectroscopy and the apparent association constant was found to be, K b = 1.68 × 10 4 M -1 and K ap =5.41 × 10 3 M -1 for DNA and BSA respectively. Addition of the titanate nano material resulted quenching of fluorescence spectra of ethidium bromide-DNA in tris HCl buffer solution and that of aqueous protein solution. The apparent binding constant (K sv = 5.46 × 10 4 M -1 for DNA binding and K sv = 6.063 × 10 3 M -1 for protein binding) was deduced from relevant fluorescence quenching data using Stern-Volmer equation.

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Spectroscopic studies on the interaction between riboflavin and albumins

Cited by 127 publications

References 37 publications

Synthesis and analysis of the anticancer activity of platinum(ii) complexes incorporating dipyridoquinoxaline variants

Synthesis and analysis of the anticancer activity of platinum(ii) complexes incorporating dipyridoquinoxaline variants

Interaction Characteristics with Bovine Serum Albumin and Retarded Nitric Oxide Release of ZCVI4-2, a New Nitric Oxide-Releasing Derivative of Oleanolic Acid

Study of Photoinduced Interaction between Calf Thymus-DNA and Bovine Serum Albumin Protein with H<sub>2</sub>Ti<sub>3</sub>O<sub>7</sub> Nanotubes

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Spectroscopic studies on the interaction between riboflavin and albumins

Cited by 127 publications

References 37 publications

Synthesis and analysis of the anticancer activity of platinum(ii) complexes incorporating dipyridoquinoxaline variants

Synthesis and analysis of the anticancer activity of platinum(ii) complexes incorporating dipyridoquinoxaline variants

Interaction Characteristics with Bovine Serum Albumin and Retarded Nitric Oxide Release of ZCVI4-2, a New Nitric Oxide-Releasing Derivative of Oleanolic Acid

Study of Photoinduced Interaction between Calf Thymus-DNA and Bovine Serum Albumin Protein with H&lt;sub&gt;2&lt;/sub&gt;Ti&lt;sub&gt;3&lt;/sub&gt;O&lt;sub&gt;7&lt;/sub&gt; Nanotubes

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Study of Photoinduced Interaction between Calf Thymus-DNA and Bovine Serum Albumin Protein with H<sub>2</sub>Ti<sub>3</sub>O<sub>7</sub> Nanotubes