Spectroscopic Study of Green Sulfur Bacteria in Stratified Water Bodies of the Kandalaksha Gulf of the White Sea

Zhiltsova, A.A.; Kharcheva, Anastasia V.; Krasnova, E. D.; Lunina, O. N.; Voronov, Dmitry A.; Savvichev, Alexander S; Gorshkova, O.M.; Patsaeva, Svetlana V.

doi:10.1134/s1024856018040188

Cited by 16 publications

(2 citation statements)

References 10 publications

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“…The conventional laboratory techniques require time consuming sampling, transportation, chromatographic separation and purification, so the original sample can be slightly or moderately damaged or modified during this period. For example, the fluorescence spectra of pigments in native form have a moderate red shift (by 10-20 nm) compared to their spectra in an organic solvent [45][46][47][48][49]. The "host" effect can affect not only the afterglow maximum position but also the quantum yield, when the pigment system of cells exhibits intermolecular migration of excitation.…”

Section: Resultsmentioning

confidence: 99%

Remote Laser Induced Fluorescence of Soils and Rocks

Lednev¹,

Бункин²,

Першин³

et al. 2021

Photonics

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The laser induced fluorescence spectroscopy was systematically utilized for remote sensing of different soils and rocks for the first time, to the best of our knowledge. Laser induced fluorescence spectroscopy measurements were carried out by the developed nanosecond LIDAR instrument with variable excitation wavelength (355, 532 and 1064 nm). LIDAR sensing of different Brazil soil samples have been carried out in order to construct a spectral database. The laser induced fluorescence spectra interpretation for different samples has been discussed in detail. The perspectives of LIDAR sensing of organic samples deposited at soils and rock have been discussed including future space exploration missions in the search for extraterrestrial life.

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Section: Resultsmentioning

confidence: 99%

Remote Laser Induced Fluorescence of Soils and Rocks

Lednev¹,

Бункин²,

Першин³

et al. 2021

Photonics

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“…Fluorescence spectroscopy has been shown to be an important tool to characterize various OM in water sources [22]. Fluorescence was linked to water quality indicators, such as pigments of anoxygenic phototrophic bacteria [23], microbial biomass and, consequently, microbial contamination [24,25].…”

Section: Introductionmentioning

confidence: 99%

Excitation-Dependent Fluorescence Helps to Indicate Fungal Contamination of Aquatic Environments and to Differentiate Filamentous Fungi

et al. 2022

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Fungal contamination of aquatic environments can lead to an adverse impact on the environment and human health. (1) The search for fast, inexpensive and appropriate methods for detection of fungi is very moving rapidly due to their significant impact on ecosystem functions and human health. (2) We focused on examination of fluorescence proxies able to distinguish chromophoric matter occurring in different fungi. Spectroscopic studies were performed on five strains of filamentous fungi: Trichoderma harzianum, Fusarium solani, Alternaria alternata, Cladosporium cladosporioides and Aspergillus terreus. (3) The results showed that most of the fungal autofluorescence was emitted by amino acids, melanin-like compounds, NAD(P)H and flavins. The spectra of five fungal species cultivated as planktonic or surface-associated forms turned out to be different. Protein fluorescence can be used to detect general microbial contamination. Presence of excitation wavelength dependent mode and the “blue shift” of fluorescence (emission bands 400–500 nm) can be suggested as specific feature of fluorescence of fungal melanin-containing samples. (4) The determination based on fluorescence spectra obtained at a certain excitation/emission wavelengths pair and at whole excitation-emission matrices (EEMs) coupled to principal component analysis (PCA) algorithms as a tool of improving detection capabilities can be suggested to enable fast and inexpensive monitoring of fungal contamination of aquatic environments.

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