Spectroscopy and Microscopy of Eco-friendly Polymer Composites

Shukla, Ashish K.; Sharma, Chhavi; Abidi, Syed M. S.; Acharya, Amitabha

doi:10.1007/978-3-030-05399-4_4

Cited by 2 publications

(2 citation statements)

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“…Plant CNCs offer a large surface area and are biocompatible and renewable for use in biomedical applications. 11,23−33 The developed nanocomposite showed promising results against HSA protein aggregation. It has previously been reported that the complex structural backbone of the cellulose is degraded by the hydrolytic action of enzymes (β- d -glucosidase and, β- d -glucuronidase) present in the macrophages, which finally convert the substrate to glucose and glucuronic acid.…”

Section: Introductionmentioning

confidence: 98%

“…In this context, the present study aimed to develop a new class of nanocomposites constituted of plant cellulose nanocrystals (CNCs) and iron oxide NPs for studying their effect on inhibition as well as dissociation of HSA aggregation. Plant CNCs offer a large surface area and are biocompatible and renewable for use in biomedical applications. ,− The developed nanocomposite showed promising results against HSA protein aggregation. It has previously been reported that the complex structural backbone of the cellulose is degraded by the hydrolytic action of enzymes (β- d -glucosidase and, β- d -glucuronidase) present in the macrophages, which finally convert the substrate to glucose and glucuronic acid .…”

Section: Introductionmentioning

confidence: 99%

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Inhibition of Glycation-Induced Aggregation of Human Serum Albumin by Organic–Inorganic Hybrid Nanocomposites of Iron Oxide-Functionalized Nanocellulose

Singla¹,

Abidi²,

Dar³

et al. 2019

ACS Omega

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Protein aggregation leads to the transformation of proteins from their soluble form to the insoluble amyloid fibrils and these aggregates get deposited in the specific body tissues, accounting for various diseases. To prevent such an aggregation, organic–inorganic hybrid nanocomposites of iron oxide nanoparticle (NP, ∼6.5–7.0 nm)-conjugated cellulose nanocrystals (CNCs) isolated from Syzygium cumini (SC) and Pinus roxburghii (PR) were chemically synthesized. Transmission electron microscopy (TEM) images of the nanocomposites suggested that the in situ-synthesized iron oxide NPs were bound to the CNC surface in a uniform and regular fashion. The ThT fluorescence assay together with 8-anilino-1-naphthalenesulfonic acid, Congo Red, and CD studies suggested that short fiber-based SC nanocomposites showed better inhibition as well as dissociation of human serum albumin aggregates. The TEM and fluorescence microscopy studies supported similar observations. Native polyacrylamide gel electrophoresis results documented dissociation of higher protein aggregates in the presence of the developed nanocomposite. Interestingly, the dissociated proteins retained their biological function by maintaining a high amount of α-helix content. The in vitro studies with HEK-293 cells suggested that the developed nanocomposite reduces aggregation-induced cytotoxicity by intracellular reactive oxygen species scavenging and maintaining the Ca2+ ion-channel. These results indicated that the hybrid organic–inorganic nanocomposite, with simultaneous sites for hydrophobic and hydrophilic interactions, tends to provide a larger surface area for nanocomposite–protein interactions, which ultimately disfavors the nucleation step for fibrillation for protein aggregates.

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Section: Introductionmentioning

confidence: 98%