Spectroscopy studies of functionalized oxidized porous silicon surface for biosensing applications

Hiraoui, Mohamed; Guendouz, Mohammed; Lorrain, Nathalie; Moadhen, A.; Haji, Lazhar; Oueslati, M.

doi:10.1016/j.matchemphys.2011.02.052

Cited by 63 publications

(49 citation statements)

References 31 publications

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“…Secondly, all of the samples have also shown the fingerprint zones of chitosan and APTES at several positions on the spectrum. The presence of Si-O-Si and Si-O-C bands at 1144 and 1110 cm −1 confirms the presence of APTES and also demonstrates the inter-linkages occurring between the Si-O and C moieties of both graphene and chitosan, respectively [41][42][43][44][45]. …”

Section: Raman Spectroscopysupporting

confidence: 57%

Study on the Effect of Silanization and Improvement in the Tensile Behavior of Graphene-Chitosan-Composite

et al. 2015

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Abstract:In the present study, silane-functionalized graphene (f-graphene)-reinforced chitosan nanocomposite films exhibiting enhanced mechanical properties have been prepared by the solution casting method. These nanocomposite films were characterized by X-ray diffraction, Raman spectroscopy and thermogravimetric analysis. In order to investigate the effect of silane functionalization, tensile tests were performed on original, oxidized and silane-functionalized graphene-reinforced chitosan nanocomposite films. Tensile results show that silane functionalization groups offer a substantial increase in the interfacial adhesion between filler and host matrix. This result is also confirmed by the surface morphology of the fracture surface in scanning electron microscope analysis. Qualitative analysis using Raman and Fourier transform infrared spectroscopy revealed the existence of Si-O-Si and Si-O-C bonds in the silanized composite. Thermal analysis of the samples shows that the material is stable up till 250 °C and maintains its thermal stability all throughout the process until it starts degrading after 510 °C. Atomic force microscopy reveals that the material is well exfoliated after the oxidation of graphene and also displays the existence of 3-6 layers of exfoliated graphene sheets. X-ray photoelectron spectroscopy studies also reveal the existence of silicon in the single state and quantify the sample to be approximately around 4% (±0.5%) of the total atomic weight. OPEN ACCESSPolymers 2015, 7 528

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Section: Raman Spectroscopysupporting

confidence: 57%

Study on the Effect of Silanization and Improvement in the Tensile Behavior of Graphene-Chitosan-Composite

et al. 2015

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“…Such a mechanism is less obvious for the terminal SiO 2 present in ToxPSi [20]. These results on surface chemical composition are directly relevant to the further biofunctionalization ( figure 8(c)): the two characteristic protein bands at 1535 and 1653 cm -1 , which are related to the N-H and C=O bonds, respectively [28,29], are stronger in CoxPSi than in ToxPSi.…”

Section: Resultsmentioning

confidence: 86%

“…After surface derivatization with APTS, a Si-O-Si band is observed at 1180 cm -1 , proving that a siloxane bond was formed between APTS and CoxPSi [19]. After biofunctionalization with immunoglobulins, two bands appear at 1535 and 1653 cm -1 related to the N-H and C=O bonds, respectively [28,29]. These chemical groups are characteristic of proteins, and their presence indicates that the surface is biofunctionalized.…”

Section: Resultsmentioning

confidence: 97%

“…The as-prepared PSi has a characteristic band at 615 cm -1 referred to as SiH. After Cox, two bands appear at every step of surface modification: a strong band at 1050-1100 cm -1 , assigned to asymmetric stretching in SiO [27], and a weak band at 835-795 cm -1 from the Si-OH bond [28]. It is clear that Cox stabilization leads to mostly oxidized PSi.…”

Section: Resultsmentioning

confidence: 99%

“…This means that ToxPSi is a harsher oxidation process than CoxPSi, as also evident from the disappearance of the SiH peak at 615 cm -1 in ToxPSi. Another important difference is the absence of the Si-OH mode at 835-795 cm -1 [28] in ToxPSi, which is important for the following derivatization step. In the FTIR spectra of derivatized surfaces ( figure 8(b)), the Si-O-Si band at 1180 cm -1 , which proves the formation of the siloxane bond [19], is weaker in ToxPSi than in CoxPSi.…”

Section: Resultsmentioning

confidence: 99%

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Chemical stabilization of porous silicon for enhanced biofunctionalization with immunoglobulin

Naveas

Costa

Gallach

et al. 2012

Science and Technology of Advanced Materials

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Porous silicon (PSi) is widely used in biological experiments, owing to its biocompatibility and well-established fabrication methods that allow tailoring its surface. Nevertheless, there are some unresolved issues such as deciding whether the stabilization of PSi is necessary for its biological applications and evaluating the effects of PSi stabilization on the surface biofunctionalization with proteins. In this work we demonstrate that non-stabilized PSi is prone to detachment owing to the stress induced upon biomolecular adsorption. Biofunctionalized non-stabilized PSi loses the interference properties characteristic of a thin film, and groove-like structures resulting from a final layer collapse were observed by scanning electron microscopy. Likewise, direct PSi derivatization with 3-aminopropyl-triethoxysilane (APTS) does not stabilize PSi against immunoglobulin biofunctionalization. To overcome this problem, we developed a simple chemical process of stabilizing PSi (CoxPSi) for biological applications, which has several advantages over thermal stabilization (ToxPSi). The process consists of chemical oxidation in H 2 O 2 , surface derivatization with APTS and a curing step at 120• C. This process offers integral homogeneous PSi morphology, hydrophilic surface termination (contact angle θ = 26• ) and highly efficient derivatized and biofunctionalized PSi surfaces (six times more efficient than ToxPSi). All these features are highly desirable for biological applications, such as biosensing, where our results can be used for the design and optimization of the biomolecular immobilization cascade on PSi surfaces.

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Nanostructured Silicon‐Based Materials as a Drug Delivery System for Water‐Insoluble Drugs

Lehto

Salonen

Santos

et al. 2013

Drug Delivery Strategies for Poorly Water‐Soluble Drugs

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Spectroscopy studies of functionalized oxidized porous silicon surface for biosensing applications

Cited by 63 publications

References 31 publications

Study on the Effect of Silanization and Improvement in the Tensile Behavior of Graphene-Chitosan-Composite

Study on the Effect of Silanization and Improvement in the Tensile Behavior of Graphene-Chitosan-Composite

Chemical stabilization of porous silicon for enhanced biofunctionalization with immunoglobulin

Nanostructured Silicon‐Based Materials as a Drug Delivery System for Water‐Insoluble Drugs

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