Spectrotemporal changes in electrical activity of myometrium due to recombinant follicle-stimulating hormone preparations follitropin alfa and beta

Çelik, Önder; Tağluk, M. Emin; Hasçalık, Şeyma; Elter, Koray; Çelik, Nilüfer; Aydın, Engin

doi:10.1016/j.fertnstert.2007.07.1391

Cited by 11 publications

(11 citation statements)

References 33 publications

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“…Consistent with this, our data show that nonpregnant human and mouse myometrium, as well as pregnant mouse myometrium through mid-gestation, respond to FSH with a quieting of contractile activity. Also, earlier studies showed that addition of FSH to nonpregnant rat myometrium suppressed myoelectrical activity [ 8 , 9 ]. Myometrium from late pregnancy that is not yet term (i.e., PD 16.5 in the mouse) exhibits an up-regulation of FSHR that is not quite as high as that seen in term nonlaboring tissue (PD 19.5).…”

Section: Discussionmentioning

confidence: 99%

“…Traditionally, FSHR expression in women was thought to be limited to the ovary. However, this receptor is increasingly appreciated to be expressed in additional tissues [ 6 – 14 ], and in several instances, such expression has been demonstrated to have unique physiological roles. For example, FSHR is expressed in osteoclasts, where FSH directly regulates bone mass [ 11 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

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Differential Regulation of Human and Mouse Myometrial Contractile Activity by FSH as a Function of FSH Receptor Density

Stilley

Guan

Santillan

et al. 2016

Biology of Reproduction

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Previous studies from our laboratory revealed that the follicle-stimulating hormone receptor (FSHR) is expressed at low levels in nonpregnant human myometrium and that it is up-regulated in pregnant term nonlaboring myometrium; however, the physiological relevance of these findings was unknown. Herein, we examined signaling pathways stimulated by FSH in immortalized uterine myocytes expressing recombinant FSHR at different densities and showed that cAMP accumulation is stimulated in all cases but that inositol phosphate accumulation is stimulated only at high FSHR densities. Because an increase in cAMP quiets myometrial contractile activity but an increase in 1,4,5-triphosphoinositol stimulates contractile activity, we hypothesized that FSHR density dictates whether FSH quiets or stimulates myometrial contractility. Indeed, in human and mouse nonpregnant myometrium, which express low levels of FSHR, application of FSH resulted in a quieting of contractile activity. In contrast, in pregnant term nonlaboring myometrium, which expresses higher levels of FSHR, application of FSH resulted in increased contractile activity. Examination of pregnant mouse myometrium from different stages of gestation revealed that FSHR levels remained low throughout most of pregnancy. Accordingly, through mid-gestation, the application of FSH resulted in a quieting of contractile activity. At Pregnancy Day (PD) 16.5, FSHR was up-regulated, although not yet sufficiently to mediate stimulation of contractility in response to FSH. This outcome was not observed until PD 19.5, when FSHR was further up-regulated. Our studies describe a novel FSHR signaling pathway that regulates myometrial contractility, and suggest that myometrial FSHR levels dictate the quieting vs. stimulation of uterine contractility in response to FSH.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Differential Regulation of Human and Mouse Myometrial Contractile Activity by FSH as a Function of FSH Receptor Density

Stilley

Guan

Santillan

et al. 2016

Biology of Reproduction

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“…Signal processing techniques provide useful information regarding uterine activity. The effectiveness of such methods has previously been discussed by our group (25). Therefore, the results obtained here have the potential to be translated into clinical therapy, as melatonin is a common product that is available as a dietary supplement that is suitable for use during pregnancy (26).…”

Section: Discussionmentioning

confidence: 71%

Dual effects of melatonin on uterine myoelectrical activity of non-pregnant rats

Şimşek¹,

Parlakpınar²,

Turhan³

et al. 2014

J Turkish German Gynecol Assoc

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(2) melatonin applied as 0.4 mg/kg/IV (n=8); (3) melatonin applied as 4 mg/kg/IV (n=8); (4) single dose of oxytocin (100 mU/kg) injected IV (n=8); (5) melatonin (0.4 mg/kg) plus oxytocin (100 mU/kg) (n=8); and (6) melatonin (4 mg/kg) plus oxytocin (100 mU/kg) injected IV (n=8). Each rat underwent a laparotomy, and uterine myoelectrical signals were recorded. The mean spectrum, averaged over the spectral content of signals in each group, was compared. Results:Melatonin induced uterine myoelectrical activity in a dose-dependent manner. Treatment of melatonin after oxytocin suppressed the mean power of the signals. Serum melatonin concentrations were significantly higher in melatonin-treated rats. Conclusion:Melatonin itself at two different dose levels was found to be equally effective in stimulating the uterine electrical signals, although oxytocin-induced uterine electrical activity was suppressed by melatonin. These findings merit further investigations on the possible beneficial role of melatonin in the treatment of conditions associated with abnormal uterine activity. (J Turk Ger Gynecol Assoc 2014; 15: 86-91) Key words: Uterine contraction, melatonin, oxytocin, electrical stimulation Received: 15 December, 2013 Accepted: 29 December, 2013 Dual effects of melatonin on uterine myoelectrical activity of non-pregnant rats

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“…The role of uterus is to provide an environment for the implantation of the embryo, so it has to go through regular cyclical changes during the oestrous cycle. Myoelectrical dynamics and/or uterine quiescence which is necessary for implantation can be affected by FSH (Celik et al, 2008;Hascalik et al, 2010). This makes the role of FSH and FSHR quite important in the establishment of pregnancy.…”

Section: Discussionmentioning

confidence: 99%

Expression and localization of follicle‐stimulating hormone receptor in the yak uterus during different stages of the oestrous cycle

Abudureyimu

Cai

Huo

et al. 2018

Reprod Domestic Animals

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Morphological changes of the uterus and alterations in its secretory activity under the influence of steroid hormones been well documented. The oestrous cycle is also associated with significant changes in plasma follicle-stimulating hormone (FSH), whose effects are mediated through its receptor (FSHR). Reports showed that in many mammals, FSHR was expressed in gonadal and extragonadal tissues including cervix, female reproductive tract, and pituitary gland. Follicle-stimulating hormone (FSH) signals through endothelial FSHR directly stimulate angiogenesis and involved in the timing of birth in human, and the presence of FSHR in the placenta is essential for normal pregnancy in mice. But little is known about FSHR expression in the yak uterus. The main objective of the present study was to determine the expression and localization of FSHR in the yak uterus during different phases of the oestrous cycle. Results showed that FSHR protein was localized in the surface and glandular epithelial cells, stroma cells, myometrial smooth muscle cells and blood vessel endothelial cells. The expression of FSHR protein peaked at oestrus, significantly decreased at dioestrus (p < 0.05) and increased again at proestrus. FSHR mRNA was highly expressed at both proestrus and oestrus, and decreased at metestrus with the lowest values at dioestrus (p < 0.05). In conclusion, FSHR expression in the yak uterus changed with the stage of the oestrous cycle suggesting that FSHR plays an essential role in regulating the endometrial and myometrial functions during the oestrus cycle in the yak.

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Spectrotemporal changes in electrical activity of myometrium due to recombinant follicle-stimulating hormone preparations follitropin alfa and beta

Cited by 11 publications

References 33 publications

Differential Regulation of Human and Mouse Myometrial Contractile Activity by FSH as a Function of FSH Receptor Density

Differential Regulation of Human and Mouse Myometrial Contractile Activity by FSH as a Function of FSH Receptor Density

Dual effects of melatonin on uterine myoelectrical activity of non-pregnant rats

Expression and localization of follicle‐stimulating hormone receptor in the yak uterus during different stages of the oestrous cycle

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