Spectrum and analysis of bone marrow findings in anemic cases

Tahlan, Anita; Bansal, Cherry; Palta, Anshu; Chauhan, Sandeep

doi:10.4103/0019-5359.42484

Cited by 10 publications

(2 citation statements)

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“…There is an obvious male preponderance (35 cases; 61.4%) against 22 cases (38.6%) in the present study. The gender distribution in most of the reported studies by Parekh [23], Bansal et al [24], Liu et al [25], and Shoket et al [26] have a male preponderance, tallying with the present findings.…”

Section: Discussionsupporting

confidence: 87%

Evaluation of Acute Leukaemias by Flow Cytometry and Its Correlation With Diagnosis Using Morphological and Special Staining Techniques

Ahmad,

Kumari,

Tirkey

et al. 2024

Cureus

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Background: Leukaemia can be reliably diagnosed and classified by the simultaneous application of multiple techniques. Cytochemical stains that are cheap and do not require any special instruments are very important in developing countries for the diagnosis of acute leukaemia (AL). Aim: To diagnose AL in all suspected cases by flow cytometry and to correlate the diagnosis with morphological and special staining like myeloperoxidase (MPO) and periodic acid-Sciff (PAS) techniques. Methods and materials: The study participants' peripheral blood smear details and bone marrow aspirate smear morphologic findings, as well as socio-demographic information, were taken from the patients' medical files. In total, 57 newly diagnosed instances of acute leukaemia confirmed by flow cytometry were incorporated into the study, which underwent cytochemical labeling and morphological diagnosis. All patients who gave previous consent had their bone marrow aspirated, and a Wright-stained smear was produced for microscopic inspection, cytochemical staining, and immunophenotyping. In an ethylenediaminetetraacetic acid (EDTA) container, peripheral blood was also drawn for the same purpose. During the entire bone marrow smear examination, we used both MPO and PAS staining techniques. Results: The study was carried out between July 2019 and June 2020. Out of 57 cases in the study, 29 (50.9%) cases on cytochemical analysis of leukaemia using PAS and MPO were diagnosed with acute myeloid leukaemia (AML) and 28 (49.1%) were diagnosed as acute lymphoid leukaemia (ALL). Cytochemical analysis of leukaemia using PAS and MPO rendered the diagnosis in 92.9% of acute leukaemia cases in our study. A total of 25 out of 25 AML cases and 28 out of 32 cases of ALL were correctly diagnosed based on morphology and cytochemical staining. Morphology and cytochemical analysis alone were unable to correctly diagnose a total of four ALL cases. All AML cases that were wrongly diagnosed as ALL were mostly M0 and M1-AML. Conclusion: Morphological staining diagnosis by itself is capable of correctly identifying a large proportion of cases of AL, which comprised 92.98% of total cases. There was also a favorable relationship between findings of diagnosis by flow cytometry and findings of diagnosis by morphology assessment in determining acute leukaemias.

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Section: Discussionsupporting

confidence: 87%

Evaluation of Acute Leukaemias by Flow Cytometry and Its Correlation With Diagnosis Using Morphological and Special Staining Techniques

Ahmad,

Kumari,

Tirkey

et al. 2024

Cureus

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“…Fever occurred in 27.3% of patients with megaloblastic anaemia, the most common aetiology being infection. In a study by Tahlan et al 23 , incidence of low-grade fever varied from 28% to 61% in patients with low iron stores and those with adequate iron stores respectively.…”

Section: Discussionmentioning

confidence: 97%

Clinico-haematological profile of children with vitamin B12 deficiency anaemia

Chauhan

Chaudhary

Nage

2020

Sri Lanka J Child Health

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Spectrum of Diseases Diagnosed by Bone Marrow Examination in Central India

Nigam¹,

Malik²,

Kothari³

et al. 2014

jemds

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BACKGROUND: Hematological diseases are quite frequent in population and mostly present with anemia. Although diagnosed mostly by clinical examination and simple investigations, bone marrow examination is confirmatory for the diagnosis. Bone marrow aspiration is simple and safe invasive procedure. Spectrum of hematological diseases in the developing countries is different from those observed in developed countries. OBJECTIVE: There is a lack of information about the exact relative prevalence of hematological disorders especially in Central India that's why this study was undertaken to correlate clinico-hematological and bone marrow findings. MATERIAL AND METHODS: It is a retrospective study of 345 cases who underwent bone marrow examination in Department Of Pathology, Gandhi Medical College, Bhopal. RESULTS: In our study age range of cases was 2-80 years with male: female ratio of 1.28:1. Most common diseases observed were megaloblastic anemia (41.81%), dimorphic anemia (16.72%), hypoplastic anemia (9.09%), ITP (8.36%) and infective pathology (4.72%). In Hematological malignancies (20.28%), most common were AML (6.95% of overall & 34.28% of malignant diseases %) followed by ALL (4.92 % overall and 24.28% % of malignancies). 3 cases of Gaucher's disease were also reported.96.81% patients were anemic while 44.92% were pancytopenic. Most common presenting symptoms were fever (44.92%) and bleeding manifestations (30.14%) which include ecchymosis, epistaxis, petechiae, melena, hematuria and hematemesis. Splenomegaly, hepatomegaly and lymphadenopathy were found in 35.65%, 21.15% and 14.78% of cases respectively. CONCLUSION: In our study final diagnosis could be made in majority of cases (92.76%) by bone marrow examination, although having varied clinical presentations such as fever, bleeding manifestations, ascites, hepatomegaly, splenomegalyetc and usually associated with anemia / pancytopenia/ thrombocytopenia.All hematological cases must undergo bone marrow examination to conclude the exact underlying pathology for appropriate treatment.

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Spectrum and analysis of bone marrow findings in anemic cases

Cited by 10 publications

References 1 publication

Evaluation of Acute Leukaemias by Flow Cytometry and Its Correlation With Diagnosis Using Morphological and Special Staining Techniques

Evaluation of Acute Leukaemias by Flow Cytometry and Its Correlation With Diagnosis Using Morphological and Special Staining Techniques

Clinico-haematological profile of children with vitamin B12 deficiency anaemia

Spectrum of Diseases Diagnosed by Bone Marrow Examination in Central India

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