Spectrum-Effect Relationship Between Antioxidant and Anti-inflammatory Effects of Banxia Baizhu Tianma Decoction: An Identification Method of Active Substances With Endothelial Cell Protective Effect

Xu, Nan; Li, Mingchen; Wang, Ping; Wang, Shuling; Shi, Haiyan

doi:10.3389/fphar.2022.823341

Cited by 11 publications

(11 citation statements)

References 25 publications

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“…Additionally, ISL promoted VEGF‐A expression in BMSCs. Similar to previous reports, ISL promoted angiogenesis in human umbilical cord endothelial cells and zebrafish (Liu et al, 2020; Xu et al, 2022; Zhang et al, 2019, 2021). In summary, ISL promoted the osteogenic differentiation and angiogenesis of BMSCs.…”

Section: Discussionsupporting

confidence: 91%

Isoliquiritin treatment of osteoporosis by promoting osteogenic differentiation and autophagy of bone marrow mesenchymal stem cells

Su,

Chen,

Huang

et al. 2023

Phytotherapy Research

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Osteoporosis is a chronic progressive bone disease characterized by the decreased osteogenic ability of osteoblasts coupled with increased osteoclast activity. Natural products showing promising therapeutic potential for postmenopausal osteoporosis remain underexplored. In this study, we aimed to analyze the therapeutic effects of isoliquiritin (ISL) on osteoporosis in mice and its possible mechanism of action. An ovariectomy‐induced osteoporosis mouse model and bone marrow mesenchymal stem cells (BMSCs) were used to analyze the effects of ISL on bone regeneration in vivo and in vitro, respectively. Mitogen‐activated protein kinase (MAPK) and autophagy inhibitors were used, to investigate whether the MAPK signaling pathway and autophagy affect the osteogenic differentiation of BMSCs. ISL significantly improved bone formation and reduced bone resorption in mouse femurs without inducing any detectable toxicity in critical organs such as the liver, kidney, brain, heart, and spleen. In vitro experiments showed that ISL enhanced the proliferation and osteogenic differentiation of BMSCs and that its osteogenic effect was attenuated by p38/extracellular regulated protein kinase (ERK) and autophagy inhibitors. Further studies showed that the inhibition of phosphorylated p38/ERK blocked ISL autophagy in BMSCs. ISL promoted the osteogenic differentiation of BMSCs through the p38/ERK‐autophagy pathway and was therapeutically effective in treating osteoporosis in ovariectomized mice without any observed toxicity to vital organs. These results strongly suggest the promising potential of ISL as a safe and efficacious candidate drug for the treatment of osteoporosis.

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Section: Discussionsupporting

confidence: 91%

Isoliquiritin treatment of osteoporosis by promoting osteogenic differentiation and autophagy of bone marrow mesenchymal stem cells

Su,

Chen,

Huang

et al. 2023

Phytotherapy Research

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“…Blume in the treatment of ischemic stroke stroke, dementia and other neurodegenerative disease (11,12). In addition, GEB Chinese patent medicine is frequently used in the clinic.…”

Section: Network Pharmacology and Molecular Docking Analysis On Molec...mentioning

confidence: 99%

“…Moreover, GEB has been used as an anticonvulsant in Asian countries for several centuries ( 10 ). Specifically, several classical formulations of GEB have been widely used in clinical practice, such as Tianma Gouteng and Banxia Baizhu Tianma decoction for treating stroke, dementia and other neurodegenerative disease ( 11 , 12 ). In addition, GEB Chinese patent medicine is frequently used in the clinic.…”

Section: Introductionmentioning

confidence: 99%

Network pharmacology and molecular docking analysis on molecular targets and mechanisms of Gastrodia elata Blume in the treatment of ischemic stroke

et al. 2022

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Gastrodia elata Blume (GEB) is widely used to treat cardio-cerebrovascular disease in China and in traditional Chinese medicine it is considered to be a dispelling wind and dredging collateral. However, the mechanism and active components of the plant in treating ischemic stroke (IS) remain unclear. The present study aimed to identify the active components and mechanism of GEB in treating IS using network pharmacology and molecular docking technology. Network analysis predicted 752 potential targets from 14 compounds in GEB, sharing 32 key targets with IS-associated targets. Gene Ontology analysis of key targets showed that 'oxidative stress', 'immune response' and 'regulation of blood circulation' were significantly enriched. Kyoto Encyclopedia of Genes and Genomes pathway analysis indicated that the key targets regulated 11 representative pathways including 'arachidonic acid metabolism', 'lipid and galactose metabolism'. In the protein-protein interaction network, five core targets, including toll-like receptor agonist, STAT3, myeloperoxidase (MPO), prostaglandin-endoperoxide synthase and matrix metalloproteinase (MMP)9, were identified and successfully docked with four active components: Palmitic acid, alexandrin, para-hydroxybenzaldehyde and gastrodin. Alexandrin, para-hydroxybenzaldehyde, and gastrodin are closely related to brain ischemia/reperfusion damage and repair. Therefore, to further verify the mechanism of action of three active components in the second part, we established the HT22 oxygen-glucose deprivation-reperfusion (OGD/R) model. Cell Counting Kit-8 assay and western blot analysis demonstrated that these three active components of GEB regulated core targets of molecular docking, such as STAT3, MPO and MMP9. In vitro experiments showed that OGD/R decreased cell survival, while this effect was reversed by the three active components of GEB. In addition, western blot analysis indicated that alexandrin upregulated expression of phosphorylated-STAT3, para-hydroxybenzaldehyde downregulated MPO and gastrodin downregulated MMP9. Therefore, the present study showed that GEB may prevent and treat IS via interaction between the active components and the main targets, which is key for investigating the efficacy of traditional Chinese medicine.

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“…For example, Tianma Gouteng drink, as a traditional Chinese medicine prescription, has been used clinically to treat cerebral infarction [ 15 ]. Banxia Baizhu Tianma decoction is another representative prescription, which has the effect of invigoration the spleen and expectoration phlegm [ 16 ]. All these pharmacological effects and functions of G. elata cannot be achieved without the active components.…”

Section: Introductionmentioning

confidence: 99%

GelFAP v2.0: an improved platform for Gene functional analysis in Gastrodia elata

Yang

et al. 2023

BMC Genomics

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Background Gastrodia elata (tianma), a well-known medicinal orchid, is widely used to treat various kinds of diseases with its dried tuber. In recent years, new chromosome-level genomes of G.elata have been released in succession, which offer an enormous resource pool for understanding gene function. Previously we have constructed GelFAP for gene functional analysis of G.elata. As genomes are updated and transcriptome data is accumulated, collection data in GelFAP cannot meet the need of researchers. Results Based on new chromosome-level genome and transcriptome data, we constructed co-expression network of G. elata, and then we annotated genes by aligning with sequences from NR, TAIR, Uniprot and Swissprot database. GO (Gene Ontology) and KEGG (Kyoto Encylopaedia of Genes and Genomes) annotations were predicted by InterProScan and GhostKOALA software. Gene families were further predicted by iTAK (Plant Transcription factor and Protein kinase Identifier and Classifier), HMMER (hidden Markov models), InParanoid. Finally, we developed an improved platform for gene functional analysis in G. elata (GelFAP v2.0) by integrating new genome, transcriptome data and processed functional annotation. Several tools were also introduced to platform including BLAST (Basic Local Alignment Search Tool), GSEA (Gene Set Enrichment Analysis), Heatmap, JBrowse, Motif analysis and Sequence extraction. Based on this platform, we found that the flavonoid biosynthesis might be regulated by transcription factors (TFs) such as MYB, HB and NAC. We also took C4H and GAFP4 as examples to show the usage of our platform. Conclusion An improved platform for gene functional analysis in G. elata (GelFAP v2.0, www.gzybioinformatics.cn/Gelv2) was constructed, which provides better genome data, more transcriptome resources and more analysis tools. The updated platform might be preferably benefit researchers to carry out gene functional research for their project.

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Spectrum-Effect Relationship Between Antioxidant and Anti-inflammatory Effects of Banxia Baizhu Tianma Decoction: An Identification Method of Active Substances With Endothelial Cell Protective Effect

Cited by 11 publications

References 25 publications

Isoliquiritin treatment of osteoporosis by promoting osteogenic differentiation and autophagy of bone marrow mesenchymal stem cells

Isoliquiritin treatment of osteoporosis by promoting osteogenic differentiation and autophagy of bone marrow mesenchymal stem cells

Network pharmacology and molecular docking analysis on molecular targets and mechanisms of Gastrodia elata Blume in the treatment of ischemic stroke

GelFAP v2.0: an improved platform for Gene functional analysis in Gastrodia elata

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