Spectrum-effect relationship for anti-tumor activity of shikonins and shikonofurans in medicinal Zicao by UHPLC-MS/MS and chemometric approaches

Liao, Mei; Pan, Yan; Liu, Xuechen; Du, Zhifeng; Jia, Shuailong; Aybek, Rahmetulla; Li, Aiqian; Kaisa, Sulaiman; Jiang, Hongliang

doi:10.1016/j.jchromb.2019.121924

Cited by 30 publications

(19 citation statements)

References 24 publications

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“…The antioxidant, antimicrobial, and other biological activities have been associated, by several authors, with the content of flavonoids and chalcones and some specific flavonoids such as pinocembrin [3][4][5][6][7][8][9]. In a previous study, the quantification of selected markers performed by HPLC-UV method, showed that the resin contains on average 3.18; 3.20; 16.04; and 12.84 g of pinocembrin (24), galangin (27), 2 ,4 -dihydroxychalcone (34) and 2 ,4 -dihydroxy-3 methoxychalcone (35) respectively, each quantified in 100 g of ZpRe [8].…”

Section: Phenolics Content Zprementioning

confidence: 99%

UHPLC-HESI-OT-MS-MS Biomolecules Profiling, Antioxidant and Antibacterial Activity of the “Orange-Yellow Resin” from Zuccagnia punctata Cav.

et al. 2020

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This research was designed to investigate the metabolite profiling, phenolics, and flavonoids content as well as the potential antioxidant and antibacterial, properties of orange-yellow resin from Zuccagnia punctata Cav (ZpRe). Metabolite profiling was obtained by a ultrahigh resolution liquid chromatography orbitrap MS analysis (UHPLC-ESI-OT-MS-MS). The antioxidant properties were screened by four methods: 2,2-diphenyl-1-picrylhydrazyl assay (DPPH), trolox equivalent antioxidant activity assay (TEAC), ferric-reducing antioxidant power assay (FRAP), and lipid peroxidation in erythrocytes (LP)). The antibacterial activity was evaluated according to the Clinical and Laboratory Standards Institute (CLSI) rules. The resin displayed a strong DPPH scavenging activity (IC 50 = 25.72 µg/mL) and showed a percentage of inhibition of LP close to that of the reference compound catechin (70% at 100 µg ZpRe/mL), while a moderated effect was observed in the FRAP and TEAC assays. The resin showed a content of phenolic and flavonoid compounds of 391 mg GAE/g and 313 mg EQ/g respectively. Fifty phenolics compounds were identified by ultrahigh resolution liquid chromatography orbitrap MS analysis (UHPLC-PDA-OT-MS) analysis. Thirty-one compounds are reported for the first time, updating the knowledge on the chemical profile of this species. The importance of the biomolecules identified support traditional use of this endemic plant. Furthermore, additional pharmacological data is presented that increase the potential interest of this plant for industrial sustainable applications.

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Section: Phenolics Content Zprementioning

confidence: 99%

UHPLC-HESI-OT-MS-MS Biomolecules Profiling, Antioxidant and Antibacterial Activity of the “Orange-Yellow Resin” from Zuccagnia punctata Cav.

et al. 2020

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“…Shikonin and its derivatives, red naphthoquinones, are widely found in the epidermis of the roots of Arnebiae Radix ( Zhan et al, 2015 ) and have been widely demonstrated to possess various biological activities, such as anti-inflammatory ( Fu et al, 2016 ; Sun et al, 2017 ; Guo et al, 2019 ), antibacterial ( Zhao et al, 2017 ; Huang et al, 2020 ), and antiangiogenic ( Liu C. et al, 2020 ) activities. Recently, it was reported that shikonin and its derivatives could induce apoptosis of many types of cancer cells and exhibit anticancer activities and antitumorigenic properties ( Liao et al, 2020 ). Amebia Radix has been widely used in the medicine, printing and dyeing industry, cosmetics and food industries ( Xu et al, 2014 ; Ma et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, high-performance liquid chromatography (HPLC) specific chromatograms, which can effectively determine the content of compounds, are widely used for authenticity confirmation and quality control of traditional Chinese medicines ( Hu et al, 2020 ; Liu B. et al, 2020 ). Some researchers have indicated that the chemical components of different Arnebiae Radix vary, some of which may have good bioactivities ( Feng et al, 2020 ; Liao et al, 2020 ; Mei et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Authentication of Three Source Spices of Arnebiae Radix Using DNA Barcoding and HPLC

Ren

et al. 2021

Front. Pharmacol.

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Arnebia decumbens (Vent.) Coss. et Kralik, A. euchroma (Royle) Johnst and A. guttata Bunge, three commonly used traditional Chinese medicinal plants have been widely used for the clinical treatment of inflammatory diseases caused by fungal, bacterial, oxidation, and other related pathogens. However, precise identification at the similar species level is usually challenging due to the influence of the source of medicinal materials, traditional ethnic medicine and medicinal habits. Here we developed a comprehensive and efficient identification system for three source spices of Arnebiae Radix based on DNA barcoding and HPLC fingerprinting. A total of 599 samples from thirty-five wild populations were collected and identified by using DNA barcodes of ITS2 regions, and the chemotypes of seven naphthoquinoneswere revealed by HPLC quantitative analysis including principal component analysis and hierarchical clustering analysis. Our results showed that the ITS2 sequences can distinguish three source spices of Arnebiae Radix from adulterants. However, it was difficult to identify them by HPLC-specific chromatograms combined with chemometric analysis. These results indicated that DNA barcoding was a more powerful method than HPLC fingerprinting for the identification of related species that were genetically similar. DNA barcoding analysis could be a promising and reliable tool to accurately confirm the identities of medicinal materials, especially for those whose sources are multiple and difficult to be identified by conventional chromatography.

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“…To clarify the components of the AMR that contribute to the inhibition activity of TYR [30,31], the fingerprints of 11 batches of AMR were established by HPLC; pharmacodynamic of TYR inhibition activity in vitro was evaluated by the biochemical enzymatic method. e effective compounds were selected by spectrum-activity relationship model which was established by correlated fingerprint peaks with pharmacodynamic data.…”

Section: Introductionmentioning

confidence: 99%

Selecting and Characterizing Tyrosinase Inhibitors from Atractylodis macrocephalae Rhizoma Based on Spectrum-Activity Relationship and Molecular Docking

Yong-qin

Liang

et al. 2021

Journal of Analytical Methods in Chemistry

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Atractylodis macrocephalae Rhizoma (AMR) is a famous classical Chinese traditional medicine (CTM), which has been used as a tonic for many diseases for thousands of years. In ancient China, it was used as a supplementary food for beauty in the palace. In preliminary studies, the function of whitening skin and the significant inhibiting effect on tyrosinase (TYR) which is the reactive enzyme in the composition of melanin of AMR were discovered, and the relevant research was rarely reported. In this study, high-performance liquid chromatography (HPLC) along with partial least squares regression analysis (PLS) was applied to survey the coherence between the chemical constituents and the inhibiting activity of 11 batches of AMR on TYR activity. The results of PLS showed that the chromatographic peaks 11 (atractylenolide III) and 15 could be important effective ingredients of the inhibition TYR activity as ascertained by spectrum-activity relationships. Furthermore, TYR inhibitory activity of atractylenolide III was validated by in vitro test by β-arbutin served as a positive control drug. The results of the in vitro test and the molecular docking showed that atractylenolide III has high TYR inhibitory activity and could link to the residues in TYR catalytic pocket. Therefore, bioassay, molecular docking, and spectrum-activity relationships are appropriate for linking the quality of samples with pharmaceutical-related active ingredients. And our studying would lay a theoretical foundation for applying the water extracts of AMR in whitening cosmetics.

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Spectrum-effect relationship for anti-tumor activity of shikonins and shikonofurans in medicinal Zicao by UHPLC-MS/MS and chemometric approaches

Cited by 30 publications

References 24 publications

UHPLC-HESI-OT-MS-MS Biomolecules Profiling, Antioxidant and Antibacterial Activity of the “Orange-Yellow Resin” from Zuccagnia punctata Cav.

UHPLC-HESI-OT-MS-MS Biomolecules Profiling, Antioxidant and Antibacterial Activity of the “Orange-Yellow Resin” from Zuccagnia punctata Cav.

Authentication of Three Source Spices of Arnebiae Radix Using DNA Barcoding and HPLC

Selecting and Characterizing Tyrosinase Inhibitors from Atractylodis macrocephalae Rhizoma Based on Spectrum-Activity Relationship and Molecular Docking

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