Spectrum of anaerobes encountered in surgical infections in a tertiary care hospital in Mangalore, coastal Karnataka: A prospective study

Antony, Beena; Justin, Sherin; Raveendran, Roshna; Shetty, Anil; Kuruvilla, Thomas S; Boloor, Rekha

doi:10.4103/0975-9727.174637

Cited by 4 publications

(9 citation statements)

References 3 publications

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“…Several international studies found such ulcers to be more common in the elderly population. [12][13][14][15] However in our study majority of the patients were young and belonged to the age group bracket of 30-40 years. This is similar to other Indian studies on diabetic foot ulcers.…”

Section: Discussionmentioning

confidence: 99%

Clinical and microbiological profile of diabetic foot ulcers in a tertiary care setup: a cross sectional analysis

et al. 2021

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Background: Diabetes associated neuropathy and vasculopathy leads to development of diabetic foot ulcers (DFU). They are responsible for considerable morbidity and a significant cost of health‐care worldwide. The magnitude of the problem becomes worse in regions where foot care is inadequate like in our country. The aim of the study to determine the microbiological profile and antimicrobial susceptibility pattern of organisms isolated from patients with diabetic foot ulcers. Methods: The study was a cross sectional study done at King George’s Medical College, Lucknow from 2012-2014 in Lucknow, Uttar Pradesh. Based on the inclusion criteria sample were collected from 112 patients. The standard protocol for the identification of microorganisms was followed. A descriptive analysis of the data was done.Results: Out of the 112 ulcers total 180 isolates were found. Of the 112 cases, 78 were monomicrobial, 32 were polymicrobial, and 2 cases were sterile on culture. The most common bacteria found was Staphylococcus aureus. Most of the staphylococcus isolated were sensitive to vancomycin, teicoplanin, cephalosporins, linezolid levofloxacin and pipercillin-tazobactum. Nearly 65% of S. aureus were methicillin‐resistant S. aureus.Conclusions: This study compiled clinical and microbiological profile of diabetic foot ulcers along with their antibiotic susceptibility patterns. Our study illuminates light on a high figure of neglected and poorly treated NHUs which could have been managed well and early treatment will prevent devastating consequences such as sepsis and amputation.

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Section: Discussionmentioning

confidence: 99%

Clinical and microbiological profile of diabetic foot ulcers in a tertiary care setup: a cross sectional analysis

et al. 2021

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“…Anaerobes are important pathogens in various human clinical infections ( 1 ). The infection caused by anaerobic organisms are often endogenous and are caused by tissue invasion of the bacteria, mostly resident near to mucosal surfaces, upper respiratory tract, gastrointestinal tract, Genitourinary tract and skin etc ( 2 ).…”

Section: Introductionmentioning

confidence: 99%

“…Most common organisms responsible for anaerobic infections are Bacteroides species, Prevotella species, Clostridium species and Cutibacterium spp. Though the anaerobes can live in complete harmony with the host and lead a mutual beneficial relationship with aerobic commensal, they can still become pathogenic when hosts defence mechanism gets impaired ( 1 ).…”

Section: Introductionmentioning

confidence: 99%

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Isolation of obligate anaerobes from clinical samples received for routine bacterial culture and sensitivity - a cross sectional study

Ayesha¹,

Gachinmath²,

Sobia³

2022

IJM

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Background and Objectives: Obligate anaerobic bacteria are known to cause various infections in human beings. We aimed to determine the prevalence and spectrum of obligate anaerobes encountered in pus aspirate, sterile fluids and tissue samples received for routine bacterial culture and sensitivity. Materials and Methods: A total of 160 samples including tissue, sterile body fluids and pus aspirate were collected , ana- lysed for prevalence and spectrum of obligate anaerobes. Identification of obligate and facultative anaerobes was done by automated MALDI-TOF and Vitek 2 method. Results: Among 160 samples, 75 samples (46.8%) yielded obligate anaerobes out of which 41 samples (26%) yielded obli- gate anaerobes along with facultative anaerobes which was significant (p value=0.031) and 34 samples (21%) yielded only obligate anaerobes. 90 obligate anaerobes were isolated from 75 samples among which only 34 (37.7%) samples yielded only obligate anaerobes and 56 (62.2%) yielded both obligate and facultative anaerobes. Gram stain with polymicrobial appear- ance (p value 0.02) was found to be significantly associated with growth of obligate anaerobes. Clinical conditions where obligate anaerobes were commonly associated were appendicular abscess, empyema, fournier’s gangrene, diabetic foot, ludwigs angina and deep abscess. Out of 75 positive samples 30 (40%) patients had predisposing conditions like diabetes mellitus, hypertension etc. Total of 90 obligate anaerobes and 49 facultative anaerobes were isolated. The common obligate anaerobes were Bacteroides fragilis 18 (20%), Prevotella spp. 20 (22.2%), and Clostridium spp. 8 (8.88%). Facultative anaerobes like Escherichia coli 25 (34.7%), Klebsiella species 15 (20.8%), Enterococcus faecalis 19 (26.3%) were isolated. Antibiotic sensitivity was performed for facultative anaerobes by Kirby bauer disc diffusion method. Out of 15 Escherichia coli isolates resistance was commonly seen for ampicillin 13 (86.6%), cephalosporins 11 (73.3%), ciprofloxacin 10 (66.6%) and Piperacillin tazobactum 8 (53.3%). In Klebsiella species resistance were commonly seen to Ampicillin 6 (100%), ceph- alosporins 2 (33.3%) and ciprofloxacin 2 (33.3%). Conclusion: There was significant isolation of obligate anaerobes along with facultative anaerobes in clinical samples re- ceived for aerobic culture and sensitivity. There is a need for isolation of these bacteria routinely and a scope for doing anti- biotic susceptibility testing, which will help in evidence-based medicine and a better clinical outcome by giving appropriate therapy.

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“…(Soki et al, 2016) Due to its ability to produce diverse infections and frequent distribution as normal flora in various mucocutaneous sites, they are accounting 60-80% of the anaerobic infection. (Antony, 2016;Barlett, 2017) They are known to produce many virulence factors. In the recent past, increased incidence of antimicrobial resistance was reported around the globe.…”

Section: …………………………………………………………………………………………………… Introduction:-mentioning

confidence: 99%

In Vitro Analysis of Virulence Factors Which Enhances Pathogenic Potential With an Emphasis on Biofilm Formation in Bacteroides Fragilis Group.

Colney¹

2019

IJAR

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Spectrum of anaerobes encountered in surgical infections in a tertiary care hospital in Mangalore, coastal Karnataka: A prospective study

Cited by 4 publications

References 3 publications

Clinical and microbiological profile of diabetic foot ulcers in a tertiary care setup: a cross sectional analysis

Clinical and microbiological profile of diabetic foot ulcers in a tertiary care setup: a cross sectional analysis

Isolation of obligate anaerobes from clinical samples received for routine bacterial culture and sensitivity - a cross sectional study

In Vitro Analysis of Virulence Factors Which Enhances Pathogenic Potential With an Emphasis on Biofilm Formation in Bacteroides Fragilis Group.

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