Speriolin is a novel human and mouse sperm centrosome protein

Goto, Masuo; O’Brien, Deborah A.; Eddy, Edward M.

doi:10.1093/humrep/deq138

Cited by 41 publications

(36 citation statements)

References 71 publications

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“…Late transcribed retained sperm transcripts encode proteins like IZUMO1 (Chalbi et al 2014) that are essential for fertilization. They also encode proteins required for early embryogenesis, e.g., centrosomal proteins, e.g., SPATC1, a sperm protein that is delivered to the oocyte and maintained after the first division (Goto et al 2010). This corresponding selective retention of sperm RNAs encoding proteins in conjunction with some of their products that are essential for fertilization and early embryogenesis is consistent with the view that their late transcription and translation in the differentiating spermatid acts to ensure their availability prior to zygotic genome activation.…”

Section: Transcripts Versus Proteinsmentioning

confidence: 99%

The protein and transcript profiles of human semen

2015

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The increasing use of "-omics" (genomic, transcriptomic, proteomic, epigenomic, and metabolomic) high-throughput measurement technologies over the past decade is beginning to reveal the complexity of human biology and physiology through the interactions of DNA, RNA, related proteins and small molecules. In reproductive medicine, the majority of this work, has thus far focused on the female factors, e.g., the oocyte, since they provide both the environment and the majority of elements required for embryogenesis. State-of-the-art sequencing and computational analyses have enabled a deeper understanding of the underlying components. Contrary to being simply a silent delivery vehicle to the oocyte of the packaged male DNA, sperm provide both a specific epigenetically marked genome together with a complex population of RNAs and proteins that are crucial for early embryogenesis. In addition to the sperm, seminal fluid appears to serve multiple roles providing a supplementary series of components that allow the sperm to successfully reach and fertilize the oocyte and prepare the female immune system to tolerate the semiallosteric embryo. A global analysis and review of what is presently known regarding the unique role of each component of the male factor and their associated interactions begins to shed light on this emergent field.

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Section: Transcripts Versus Proteinsmentioning

confidence: 99%

The protein and transcript profiles of human semen

2015

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“…SPATC1L-This speriolin-like protein is of unknown function, whereas speriolin is a spermatogenic cell-specific centrosomal protein (50). Before activation, CFP-SPATC1L was distributed in the cytoplasm, the nucleus and in the perinuclear region.…”

Section: Identification Of Fluorescently Labeled Proteins That Responmentioning

confidence: 99%

A Fluorescent Live Imaging Screening Assay Based on Translocation Criteria Identifies Novel Cytoplasmic Proteins Implicated in G Protein-coupled Receptor Signaling Pathways

Lecat

Matthes

Pepperkok

et al. 2015

Molecular & Cellular Proteomics

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Eukaryotic cells have evolved to segregate cellular functions into specialized intracellular membrane compartments. Information regarding the precise intracellular localization of a poorly characterized protein is thus important when evaluating its function. However, localization per se, without additional information, is not sufficient to indicate the potential function of cytoplasmic, or cytoplasmic and nuclear proteins that account for almost half of the proteome diversity, when compared with proteins located in dedicated organelles, such as the Golgi apparatus or lysosomes. To tackle this need for additional information, we have implemented a live-imaging screening strategy that is based on changes in localization of fluorescently labeled cytoplasmic proteins studied upon the activation of cells. Indeed, cytoplasmic and nuclear proteins often respond to the activation of a given signaling cascade by translocating to another cell compartment, for instance by moving from the cytoplasm to the plasma membrane, or to the nucleus. Classical signaling proteins that translocate following receptor activation include AKT/PKB (1), raf1 (2), or NF-KB (3). Therefore, finding that a given cytoplasmic protein responds to the activation of a given signaling cascade provides further insight into its function.

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“…Other proteins in the mature sperm are only incompletely understood although new studies have started to focus on this topic. Recently, Goto et al (2010) reported that speriolin is present in sperm of human and mouse oocytes and is contributed to the oocyte after fertilization; it is detected at the mitotic poles in both systems despite the fact that in the mouse, the sperm centriole is destroyed and does not participate in the fertilization process (Schatten et al 1985a). One complexity regarding sperm centrosomal components relates to the fact that many previous studies have used immunofluorescence microscopy without extensive control experiments to possibly unmask antigens that may not be detected because of inaccessibility to the antibody.…”

Section: Introductionmentioning

confidence: 99%

New insights into the role of centrosomes in mammalian fertilization and implications for ART

Schatten

Sun²

2011

Reproduction

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In non-rodent mammalian species, including humans, the oocyte and sperm both contribute centrosomal components that are most important for successful fertilization. Centrosome pathologies in sperm and the oocyte can be causes for infertility which may be overcome by assisted reproductive technologies based on proper diagnosis of specific centrosomal pathologies. However, we do not yet fully understand the cell and molecular mechanisms underlying centrosome functions in germ cells and in the developing embryo, which calls for directed specific investigations to identify centrosome-related pathologies that include components in sperm, egg, or centrosome regulation within the fertilized oocyte. The present review highlights cellular and molecular aspects of centrosomes and centrosome-nuclear interactions focused on nuclear mitotic apparatus protein during fertilization and proposes future directions in expanding therapeutic approaches related to centrosome pathologies that may play a role in still unexplained causes of infertility.

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Speriolin is a novel human and mouse sperm centrosome protein

Abstract: Speriolin is a novel centrosomal protein present in the connecting piece region of mouse and human sperm that is transmitted to the mouse zygote and can be detected throughout the first mitotic division.

Cited by 41 publications

References 71 publications

The protein and transcript profiles of human semen

The protein and transcript profiles of human semen

A Fluorescent Live Imaging Screening Assay Based on Translocation Criteria Identifies Novel Cytoplasmic Proteins Implicated in G Protein-coupled Receptor Signaling Pathways

New insights into the role of centrosomes in mammalian fertilization and implications for ART

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