Sperm aging in utero and chromosomal anomalies in rabbit blastocysts

Chromosome Abnormalities in Rabbit Preimplantation Blastocysts Induced by Superovulation

“…The ovulation rates in those reports, however, were 10-5 (287/27) and 10-0 (110/10), respec¬ tively; while that of our present data was 22 (199/9), which was assumed to be superovulatory. The percentage recovery (number of observed blastocysts) in the superovulation group (73-9%) was less than in the control group (86-9%) and also less than the levels reported by Martin & Shaver (1972) and Widmeyer & Shaver (1972) and might be due to the effect of the superovulation (Table 1).…”

Sperm age, sex ratio, and hyperhaploidy frequency in mice

“…James (1971) and Guerrero (1974) reported that when insemination occurs early in the men strual cycle-with the sperm aging in the female genital tractmale progeny are more likely to result. Further evidence comes from Martin and Shaver's (1972) experiments, which demon strated a similar effect in rabbit blastocysts. Finally, analysis of human sperm chromosomes through the use of the hamster Supported by a National Institutes of Health-Biomed grant to P.A.M.-D.…”

A Preliminary Note on Chromosome Abnormalities of Intratubal Rabbit Embryos

“…The reported incidence of chromosomal anomalies in rabbit blastocysts to which gonadotropins were not administered ranged from 0 to 1.7% (Shaver and Carr 1969;Widmeyer and Shaver 1972;Fujimoto et al 1974a). In contrast, considerably increased incidence ranging from 1.0 to 12.0% occurred after administration of various doses of gonadotropins (Shaver and Carr 1969;Shaver 1970;Hofsaess and Meacham 1971;Martin and Shaver 1972;Fechheimer and Beatty 1974;Fujimoto et al 1974a). The same tendency was shown in the present intratubal embryos, although the number of embryos analysed was not large enough.…”

“…There are cytogenetic studies on pre-implantation blastocysts of the rabbit (Shaver and Carr 1967;Austin 1967;Shaver 1970;Hof saess and Meacham 1971;widmeyer and Shaver 1972;Martin and Shaver 1972;Fechheimer and Beatty 1974;Fujimoto et al 1974a), but little has been known on the chromosomes of very early intratubal embryos. This paper describes preliminarily some chromosomal aspects of the intratubal rabbit embryos with an improved double fixation method (Fujimoto et al 1974b), with attention to the effect of superovulation on the chromosomes of rabbit intratubal embryos at the 8-to 16-cell stage.…”