Sperm Capacitation, the Acrosome Reaction, and Fertilization

Šutovský, Peter

doi:10.1007/978-1-4419-1436-1_25

Cited by 2 publications

(2 citation statements)

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“…However, a recent capillary-cumulus model found that acrosome-reacted sperm could penetrate the cumulus in the capillary instead of trapping in the cumulus mass (Rijsdijk and Franken, 2007). It has also been observed that the dispersal of acrosome content persists at least for 15 min after the initiation of AR in vitro, indicating that the status of acrosome is neither OFF (intact) nor ON (acted), but includes a series of intermediate states (Hardy et al, 1991;Kim et al, 2001;Kim and Gerton, 2003;Sutovsky et al, 2010). Moreover, this model fails to explain why there exists such a large percentage of false or spontaneous acrosome-reacted sperm in almost all species examined so far.…”

Section: Status Of Sperm Acrosome In the Cumulus: The Controversymentioning

confidence: 99%

Acrosome reaction in the cumulus oophorus revisited: involvement of a novel sperm-released factor NYD-SP8

2011

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Fertilization is a process involving multiple steps that lead to the final fusion of one sperm and the oocyte to form the zygote. One of the steps, acrosome reaction (AR), is an exocytosis process, during which the outer acrosome membrane fuses with the inner sperm membrane, leading to the release of acrosome enzymes that facilitate sperm penetration of the egg investments. Though AR has been investigated for decades, the initial steps of AR in vivo, however, remain largely unknown. A well elucidated model holds the view that AR occurs on the surface of the zona pellucida (ZP), which is triggered by binding of sperm with one of the ZP glycosylated protein, ZP3. However, this model fails to explain the large number of 'falsely' acrosome-reacted sperms found within the cumulus layer in many species examined. With the emerging evidence of cross-talk between sperm and cumulus cells, the potential significance of AR in the cumulus oophorus, the outer layer of the egg, has been gradually revealed. Here we review the acrosome status within the cumulus layer, the cross-talk between sperm and cumulus cells with the involvement of a novel spermreleased factor, NYD-SP8, and re-evaluate the importance and physiological significance of the AR in the cumulus in fertilization.

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Section: Status Of Sperm Acrosome In the Cumulus: The Controversymentioning

confidence: 99%

Acrosome reaction in the cumulus oophorus revisited: involvement of a novel sperm-released factor NYD-SP8

2011

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“…Conservation of this expression on the acrosomal cap suggests a function similar to that observed in other species [11,28]. The acrosomal cap is involved in capacitation, sperm-zona pellucida binding, and acrosomal exocytosis and stores calcium within the sperm [29,30]. In humans and bovine species, Sia has been implicated in sperm-oocyte binding and oocyte penetration [31][32][33], although specific sperm ligands have not been identified.…”

Section: Discussionmentioning

confidence: 84%

Comparison of Siglec-1 protein networks and expression patterns in sperm and male reproductive tracts of mice, rats, and humans

Almhanna,

Kumar,

Kilroy

et al. 2024

Vet World

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Background and Aim: Sialic acid-binding immunoglobulin-like lectin 1 (Siglec-1) is a transmembrane glycoprotein involved in the sialic acid (Sia)-dependent regulation of the immune system. Siglec-1 expression has recently been identified in the male reproductive tract (MRT) of several species, including humans, cattle, horses, and sheep, and may play a role in modulating fertility in a Sia-dependent manner. Materials and Methods: In this study, proteinâ€“protein interaction (PPI) analysis of Siglec-1 was conducted to identify associated network protein conservation, and the expression of Siglec-1 in the MRT of mice and rats, including their accessory sex glands and spermatozoa was determined by immunostaining. Results: Network analysis of proteins with Siglec-1 in mice and rats demonstrated significant similarity to human Siglec-1 networks, suggesting a similar conservation of network proteins between these species and, hence, a potential conservation role in immune modulation and function. Specific immunostaining patterns of mouse and rat testes, epididymis, ductus deferens, accessory sex gland tissues, and sperm were detected using human Siglec-1. These results confirmed that the human Siglec-1 antibody could cross-react with mouse and rat Siglec-1, suggesting that the specific expression patterns of Siglec-1 in the MRT and sperm of both mice and rats are similar to those observed in other species. Conclusion: The conservation of Siglec-1 expression patterns in sperm and within the MRT and the similarity of protein networks for Siglec-1 across species suggest that Siglec-1 may function in a similar manner across species. These results also suggest that rodents may serve as a valuable model system for exploring the function of Siglecs in the reproductive system across species and their potential role in modulating fertility in a Sia-dependent manner. Keywords: female reproductive tract, male reproductive tract, proteinâ€“protein interaction, sialic acid, sialic acid-binding immunoglobulin-like lectin-1.

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Sperm Capacitation, the Acrosome Reaction, and Fertilization

Cited by 2 publications

References 408 publications

Acrosome reaction in the cumulus oophorus revisited: involvement of a novel sperm-released factor NYD-SP8

Acrosome reaction in the cumulus oophorus revisited: involvement of a novel sperm-released factor NYD-SP8

Comparison of Siglec-1 protein networks and expression patterns in sperm and male reproductive tracts of mice, rats, and humans

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